Open Access

miR‑208b‑5p inhibits invasion of non‑small cell lung cancer through the STAT3 pathway by targeting interleukin‑9

  • Authors:
    • Jun Ma
    • Hong‑Feng Tong
    • Jie‑Huan Lin
    • Fu‑Nan Chen
    • Can‑Xing Wu
    • Cheng‑Zhang Cao
    • Jian Wu
    • Shu‑Qiao Hu
  • View Affiliations

  • Published online on: April 23, 2020     https://doi.org/10.3892/ol.2020.11570
  • Pages: 299-307
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies reported a dysregulation of micro (mi)R-208b-5p expression level in various types of human cancer; however, the role of miR‑208‑5p in non‑small cell lung cancer (NSCLC) remains unclear. Therefore, the present study aimed to determine whether miR‑208b‑5p could regulate NSCLC progression. A total of 62 pairs of primary tumor and adjacent normal tissues were collected from patients with NSCLC. miR‑208b‑5p expression level was determined by reverse transcription‑quantitative polymerase chain reaction. Furthermore, miR‑208b‑5p mimics was transfected into NSCLC A549 and H1299 cells in order to upregulate miR‑208b‑5p expression. Dual‑luciferase reporter assay was utilized to investigate the associations between miR‑208b‑5p and IL9 mRNA. The results demonstrated that miR‑208b‑5p expression decreased in NSCLC tissues and cell lines. Furthermore, miR‑208b‑5p overexpression inhibited A549 and H1299 cell proliferation and invasiveness. miR‑208b‑5p was demonstrated to bind directly to the 3' untranslated region of interleukin‑9 (IL‑9) and therefore decreased its expression. In the NSCLC‑derived cell lines, miR‑208b‑5p inactivated IL‑9/signal transducer and activator of transcription 3 (STAT3) signaling pathway. Furthermore, enhanced IL‑9 level decreased the miR‑208b‑5p‑mediated suppression of epithelial‑mesenchymal transition in NSCLC cells by inactivating the STAT3 signaling pathway. In conclusion, the findings from this study demonstrated that miR‑208b‑5p inhibited migration and invasion of NSCLC cells. The anti‑tumor activity of miR‑208b‑5p may be mediated by IL‑9 and STAT‑3 pathway.

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July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Ma, J., Tong, H., Lin, J., Chen, F., Wu, C., Cao, C. ... Hu, S. (2020). miR‑208b‑5p inhibits invasion of non‑small cell lung cancer through the STAT3 pathway by targeting interleukin‑9. Oncology Letters, 20, 299-307. https://doi.org/10.3892/ol.2020.11570
MLA
Ma, J., Tong, H., Lin, J., Chen, F., Wu, C., Cao, C., Wu, J., Hu, S."miR‑208b‑5p inhibits invasion of non‑small cell lung cancer through the STAT3 pathway by targeting interleukin‑9". Oncology Letters 20.1 (2020): 299-307.
Chicago
Ma, J., Tong, H., Lin, J., Chen, F., Wu, C., Cao, C., Wu, J., Hu, S."miR‑208b‑5p inhibits invasion of non‑small cell lung cancer through the STAT3 pathway by targeting interleukin‑9". Oncology Letters 20, no. 1 (2020): 299-307. https://doi.org/10.3892/ol.2020.11570