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Article

Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer

  • Authors:
    • Wei Yin
    • Xiang Wang
    • Yunping Li
    • Bin Wang
    • Mingzhe Song
    • Alicia Hulbert
    • Chen Chen
    • Fenglei Yu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Surgery, University of Illinois at Chicago School of Medicine, Chicago, IL 60607, USA
  • Pages: 967-973
    |
    Published online on: May 6, 2020
       https://doi.org/10.3892/ol.2020.11592
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Abstract

In the present study, promoter hypermethylation of cysteine dioxygenase type 1 (CDO1) was evaluated in non‑small cell lung cancer (NSCLC) tissues to assess the value of CDO1 as a novel biomarker to improve the diagnosis of NSCLC. Tumor tissue samples and corresponding normal lung tissue samples from 42 patients with NSCLC were obtained at the Department of Thoracic Surgery, The Second Xiangya Hospital (Changsha, China). Conventional methylation‑specific PCR (cMSP) and methylation‑on‑beads followed by quantitative methylation‑specific PCR (MOB‑qMSP) were used to analyze the tumor and normal lung tissue samples. Using these two methods, promoter DNA hypermethylation of the CDO1 gene was detected in 59.4 and 71.0% of tumor tissues of patients with NSCLC and in 9.4 and 0% of normal lung tissue, respectively. Compared with the rate of methylation in the well‑differentiated NSCLC tissues (15.4 and 55.6%, respectively), the rate of CDO1 gene promoter methylation was higher in the poorly differentiated tissues (89.5 and 92.3%, respectively). Overall, it was demonstrated that the MOB‑qMSP method had a higher positive detection rate for CDO1 hypermethylation compared with the cMSP method. In conclusion, CDO1 gene promoter hypermethylation was more frequently observed in NSCLC tissues compared with in normal lung tissues, and a high methylation frequency of the CDO1 gene in biopsy specimens of NSCLC was associated with the degree of differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Yin W, Wang X, Li Y, Wang B, Song M, Hulbert A, Chen C and Yu F: Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer. Oncol Lett 20: 967-973, 2020.
APA
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A. ... Yu, F. (2020). Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer. Oncology Letters, 20, 967-973. https://doi.org/10.3892/ol.2020.11592
MLA
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A., Chen, C., Yu, F."Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer". Oncology Letters 20.1 (2020): 967-973.
Chicago
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A., Chen, C., Yu, F."Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer". Oncology Letters 20, no. 1 (2020): 967-973. https://doi.org/10.3892/ol.2020.11592
Copy and paste a formatted citation
x
Spandidos Publications style
Yin W, Wang X, Li Y, Wang B, Song M, Hulbert A, Chen C and Yu F: Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer. Oncol Lett 20: 967-973, 2020.
APA
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A. ... Yu, F. (2020). Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer. Oncology Letters, 20, 967-973. https://doi.org/10.3892/ol.2020.11592
MLA
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A., Chen, C., Yu, F."Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer". Oncology Letters 20.1 (2020): 967-973.
Chicago
Yin, W., Wang, X., Li, Y., Wang, B., Song, M., Hulbert, A., Chen, C., Yu, F."Promoter hypermethylation of cysteine dioxygenase type 1 in patients with non‑small cell lung cancer". Oncology Letters 20, no. 1 (2020): 967-973. https://doi.org/10.3892/ol.2020.11592
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