Open Access

miR‑203 affects esophageal cancer cell proliferation, apoptosis and invasion by targeting MAP3K1

  • Authors:
    • Mingzhu Zong
    • Wanting Feng
    • Li Wan
    • Xiaojuan Yu
    • Weiyong Yu
  • View Affiliations

  • Published online on: May 13, 2020     https://doi.org/10.3892/ol.2020.11610
  • Pages: 751-757
  • Copyright: © Zong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

miR-203 has been indicated to be a tumor suppressor in esophageal cancer, however, the underlying molecular mechanisms by which it functions are not fully understood. The present study aimed to investigate the molecular mechanisms underlying the regulatory activities of microRNA (miR)‑203 in esophageal cancer. The miR‑203 mimic/inhibitor, Mitogen‑Activated Protein Kinase Kinase Kinase 1 (MAP3K1) overexpression plasmid and MAP3K1 small interfering (si)RNA were transfected into TE‑1 cells. miR‑203 and MAP3K1 mRNA expression were detected via reverse transcription‑quantitative PCR analysis, while MAP3K1 protein expression was detected via western blot analysis. Dual‑luciferase reporter assay was used to determine whether MAP3K1 was a direct target of miR‑203. Cell proliferation and invasion abilities were assessed via MTT and Matrigel assays, respectively. Cell apoptosis was analyzed via flow cytometry, Caspase 8/3 Assay kits and western blot analysis. The results demonstrated that MAP3K1 was a direct target of miR‑203. Overexpression of MAP3K1 reversed the suppressed cell proliferation and invasion abilities induced by miR‑203 mimic, as well as the inhibitory effect of miR‑203 mimic on cell apoptosis. Furthermore, MAP3K1 siRNA weakened the effect of miR‑203 inhibitor on cell proliferation, apoptosis and invasion.

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July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Zong, M., Feng, W., Wan, L., Yu, X., & Yu, W. (2020). miR‑203 affects esophageal cancer cell proliferation, apoptosis and invasion by targeting MAP3K1. Oncology Letters, 20, 751-757. https://doi.org/10.3892/ol.2020.11610
MLA
Zong, M., Feng, W., Wan, L., Yu, X., Yu, W."miR‑203 affects esophageal cancer cell proliferation, apoptosis and invasion by targeting MAP3K1". Oncology Letters 20.1 (2020): 751-757.
Chicago
Zong, M., Feng, W., Wan, L., Yu, X., Yu, W."miR‑203 affects esophageal cancer cell proliferation, apoptosis and invasion by targeting MAP3K1". Oncology Letters 20, no. 1 (2020): 751-757. https://doi.org/10.3892/ol.2020.11610