Open Access

Wide‑ranging analysis of survival‑related alternative splicing events in invasive breast carcinoma

  • Authors:
    • Keren Jia
    • Yingcheng Wu
    • Jing Huang
    • Jianing Chen
    • Huagen Wei
    • Huiqun Wu
  • View Affiliations

  • Published online on: June 5, 2020     https://doi.org/10.3892/ol.2020.11695
  • Pages: 1866-1878
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Invasive breast carcinoma (BRCA) is a serious disease that threatens the survival time of those affected. Alternative splicing (AS) involved in BRCA pathogenesis may be a potential therapeutic target. However, to the best of our knowledge, a systematic analysis of survival‑related alternative splicing events (SREs) has not yet been reported. The aim of the present study was to identify SREs and analyze their potential biological functions as BRCA prognostic biomarkers. An UpSet plot demonstrated AS global characteristics. Cox's proportional hazards regression model quantitatively demonstrated the prognostic relevance of AS events. Functional enrichment analysis investigated the potential pathways through which AS events affect BRCA progression. The receiver operating characteristic curve model determined the clinical significance of AS events represented using percent‑spliced‑in (PSI) values. The regulatory network of splicing factors (SFs) and AS events laid the foundation for studying the role of SFs in BRCA. The present study identified 1,215 SREs and their distribution characteristics, suggesting that AS events in exon skipping (ES) primarily exerted normal physiological functions, while AS events in alternative terminator sites had the most significant prognostic effect. The present study demonstrated that survival‑associated genes are involved primarily in certain biological processes of ribosomal proteins. In the diagnostic model, the alternative acceptor site, alternative donor site, alternative promoter site and ES performed well. ELAVL4 was the key gene associated with prognosis and SREs. In conclusion, a number of AS events affect BRCA initiation, progression and prognosis. The PSI value of AS events has the potential to diagnose BRCA and predict a prognosis; however, this must be confirmed in additional studies.

Related Articles

Journal Cover

August-2020
Volume 20 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Jia, K., Wu, Y., Huang, J., Chen, J., Wei, H., & Wu, H. (2020). Wide‑ranging analysis of survival‑related alternative splicing events in invasive breast carcinoma. Oncology Letters, 20, 1866-1878. https://doi.org/10.3892/ol.2020.11695
MLA
Jia, K., Wu, Y., Huang, J., Chen, J., Wei, H., Wu, H."Wide‑ranging analysis of survival‑related alternative splicing events in invasive breast carcinoma". Oncology Letters 20.2 (2020): 1866-1878.
Chicago
Jia, K., Wu, Y., Huang, J., Chen, J., Wei, H., Wu, H."Wide‑ranging analysis of survival‑related alternative splicing events in invasive breast carcinoma". Oncology Letters 20, no. 2 (2020): 1866-1878. https://doi.org/10.3892/ol.2020.11695