miR‑9‑3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

  • Authors:
    • Jianwen Zhen
    • Hengxun Zhang
    • Hongzhi Dong
    • Xiaopeng Tong
  • View Affiliations

  • Published online on: June 11, 2020     https://doi.org/10.3892/ol.2020.11725
  • Pages: 2007-2015
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

There is accumulating evidence indicating that microRNA (miR)‑9‑3p expression is abnormal in patients with glioma; however, the role of miR‑9‑3p in glioma remains unclear. In the present study, reverse transcription‑quantitative PCR and immunohistochemical assays were conducted to assess miR‑9‑3p and forkhead box G1 (FOXG1) expression, respectively. A luciferase reporter assay was performed to confirm the target of miR‑9‑3p. Moreover, cell counting kit‑8 and flow cytometry assays were used to assess proliferation and apoptosis, respectively. The present study demonstrated that miR‑9‑3p is significantly downregulated, and FOXG1 is significantly upregulated, in patients with glioma. miR‑9‑3p overexpression inhibited proliferation and increased the apoptosis of both U87MG and TG‑905 cells. In addition, FOXG1 was identified as a direct target of miR‑9‑3p, and FOXG1 silencing enhanced the inhibitory effect of miR‑9‑3p on proliferation and apoptosis in U87 MG and TG‑905 cells. In conclusion, the present results suggest that miR‑9‑3p may suppress malignant biological properties by targeting FOXG1. Thus, miR‑9‑3p may serve as a diagnostic target and novel prognostic marker in patients with glioma.

Related Articles

Journal Cover

August-2020
Volume 20 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Zhen, J., Zhang, H., Dong, H., & Tong, X. (2020). miR‑9‑3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1. Oncology Letters, 20, 2007-2015. https://doi.org/10.3892/ol.2020.11725
MLA
Zhen, J., Zhang, H., Dong, H., Tong, X."miR‑9‑3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1". Oncology Letters 20.2 (2020): 2007-2015.
Chicago
Zhen, J., Zhang, H., Dong, H., Tong, X."miR‑9‑3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1". Oncology Letters 20, no. 2 (2020): 2007-2015. https://doi.org/10.3892/ol.2020.11725