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Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél

  • Authors:
    • Mingming Dong
    • Yiling Hou
    • Xiang Ding
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China, College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan 637009, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2169-2182
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    Published online on: June 19, 2020
       https://doi.org/10.3892/ol.2020.11761
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Abstract

It is an important aspect of current cancer research to search for effective and low‑toxicity anticancer drugs and adjuvants. Polysaccharides, as immunomodulators, can improve the immune function of the body, kill tumor cells directly and prevent tumor development by increasing the resistance of the body to carcinogenic factors. The aim of the present study was to identify natural polysaccharide compounds with novel structure and antitumor activity via the separation and analysis of polysaccharide components from Ramaria flaccida (Fr.) Quél. (RF‑1). In the present study, high‑performance gel permeation chromatography, gas chromatography‑mass spectrometry and nuclear magnetic resonance were used to identify the structure of polysaccharides from RF‑1. Subsequently, the antitumor activity and mechanism of RF‑1 were studied by establishing an in vivo S180 tumor model, and by using Illumina sequencing technology and enzyme‑linked immunosorbent assay (ELISA). The present results revealed that the average molecular weight of RF‑1 was 17,093 Da and that RF‑1 was composed of the monosaccharides glucose and galactose, with a 2:1 ratio. The main chain of RF‑1 consisted of (1→6, 2)‑α‑D‑galactopyranose and (1→6, 4)‑​α‑D‑glucopyranose. One of the branched chains was linked to 4‑O of the main glucose chain by (1→6)‑α‑D‑glucopyranose and next linked by one (→4)‑β‑D‑glucopyranose. The other two branched chains were both linked to 2‑O of the main glucose chain by one (→4)‑β‑D‑glucopyranose. In addition, RF‑1 inhibited the growth of S180 tumors in vivo. When the concentration of RF‑1 was 20 mg/kg, the inhibition rate of S180 tumors in mice was 48.4%. Compared with the blank control group, 1,971 differentially expressed genes were identified, of which 818 were upregulated and 1,153 were downregulated in the RF‑1 group. A Gene Ontology enrichment analysis generated 47,091 assignments to biological processes, 5,250 assignments to cellular components, and 6,466 assignments to molecular functions. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the Wnt and MAPK signaling pathways were significantly enriched. The number of differentially annotated genes in these two pathways was 19 and 33, respectively. Additionally, ELISA results revealed that the protein levels of interleukin (IL)‑1β, IL‑6, vascular endothelial growth factor (VEGF) and VEGF receptor in the RF‑1 group were significantly downregulated compared with the S180 blank control group (P<0.01).
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View References

1 

Yang H, Guo S and Sagitov A: The collection and separation of the special edible and medicinal mushrooms in china and Kazakhstan and efficacy evaluation. Sci Tech Infor Dev Economy. 23:132–136. 2013.

2 

Zheng L, Chen XQ and Cheong KL: Current trends in marine algae polysaccharides: The digestive tract, microbial catabolism, and prebiotic potential. Int J Biol Macromol. 151:344–354. 2020. View Article : Google Scholar : PubMed/NCBI

3 

Liu L, Cui Y, Pi F, Cheng Y, Guo Y and Qian H: Extraction, purification, structural characteristics, biological activities and pharmacological applications of acemannan, a polysaccharide from aloe Vera: A review. Molecules. 24:15542019. View Article : Google Scholar

4 

Ding X, Hou YL and Hou WR: Structure feature and antitumor activity of a novel polysaccharide isolated from Lactarius deliciosus Gray. Carbohydr Polym. 89:397–402. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Yu Y, Shen MY, Song QQ and Xie JH: Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review. Carbohydr Polym. 183:91–101. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Chen Q, Cao M, Xiang WL, Sun Q, Zhang J, Hou RT, Yan ZY, Yang ZR, Liu J and Zhao J: Study on genes with altered expression in alpha-amanitin poisoned mice and evaluation antagonistic effects of traditional Chinese medicines against its toxicity. Acta Biol Hung. 60:281–291. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Hou YL, Liu L, Ding X, Zhao DQ and Hou WR: Structure elucidation, proliferation effect on macrophage and its mechanism of a new heteropolysaccharide from Lactarius deliciosus Gray. Carbohydr Polym. 152:648–657. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Zhang X, Aweya JJ, Huang ZX, Kang ZY, Bai ZH, Li KH, He XT, Liu Y, Chen XQ and Cheong KL: In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota. Carbohydr Polym. 234:1158942020. View Article : Google Scholar : PubMed/NCBI

9 

Song G and Du Q: Structure characterization and antitumor activity of an α β-glucan polysaccharide from auricularia polytricha. Food Res Int. 45:381–387. 2012. View Article : Google Scholar

10 

Kumar S and Gautam N: Chemical and bioactive profiling, and biological activities of coral fungi from northwestern Himalayas. Sci Rep. 7:465702017. View Article : Google Scholar : PubMed/NCBI

11 

Su S, Yang Q and Li C: Parameter optimization of extraction technology of polysaccharides from Ramaria flaccida. Nat Prod Res Dev. 23:751–754. 2011.

12 

Li H, Dou X and Lu Q: Optimization of selected parameters affecting the extraction of DPPH radical-scavenging polysaccharide from Ramaria botrytis fruit bodies. Acta Edulis Fungi. 19:69–72. 2012.

13 

Ruthes AC, Smiderle FR and Iacomini M: D-glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches. Carbohydr Polym. 117:753–761. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Bin Y, Ying Y, Xuefei W, Yanqing HU, Linlin F and Dandan L: Optimization of deproteinized process from echinops latifolius tausch polysaccharide by response surface methodology. Sci Technol Food Industry. 35:287–291. 2014.

15 

Plancot B, Gügi B, Mollet JC, Loutelier-Bourhis C, Ramasandra GS, Lerouge P, Follet-Gueye ML, Vicré M, Alfonso C, Nguema-Ona E, et al: Desiccation tolerance in plants: Structural characterization of the cell wall hemicellulosic polysaccharides in three Selaginella species. Carbohydr Polym. 208:180–190. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Pandya U, Dhuldhaj U and Sahay NS: Bioactive mushroom polysaccharides as antitumor: An overview. Nat Prod Res. 33:2668–2680. 2019. View Article : Google Scholar : PubMed/NCBI

17 

Mohan M, Achary A, Mani V, Cicinskas E, Kalitnik AA and Khotimchenko M: Purification and characterization of fucose-containing sulphated polysaccharides from Sargassum tenerrimum and their biological activity. J App Phycol. 1:1–13. 2019.

18 

Mannino MR and Orecchio S: Polycyclic aromatic hydrocarbons (PAHs) in indoor dust matter of Palermo (Italy) area: Extraction, GC-MS analysis, distribution and sources. Atmospheric Environment. 42:1801–1817. 2008. View Article : Google Scholar

19 

Sathivel A, Raghavendran HB, Srinivasan P and Devaki T: Anti-peroxidative and anti-hyperlipidemic nature of Ulva lactuca crude polysaccharide on D-Galactosamine induced hepatitis in rats. Food Chem Toxicol. 46:3262–3267. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Šilhánek J: Comparisons of the most important chemistry databases-Scifinder program and reaxys database system. Chemicke Listy. 108:81–106. 2014.

21 

Gao B and Yang GZ: Effects of Ganoderma applanatum polysaccharide on cellular and humoral immunity in normal and sarcoma 180 transplanted mice. Phytotherapy Res. 5:134–138. 1991. View Article : Google Scholar

22 

Duan DP, Dang XQ, Wang KZ, Wang YP, Zhang H and You WL: The cyclooxygenase-2 inhibitor NS-398 inhibits proliferation and induces apoptosis in human osteosarcoma cells via downregulation of the survivin pathway. Oncol Rep. 28:1693–1700. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Parkhomchuk D, Borodina T, Amstislavskiy V, Banaru M, Hallen L, Krobitsch S, Lehrach H and Soldatov A: Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37:e1232009. View Article : Google Scholar : PubMed/NCBI

24 

Daehwan K, Ben L and Steven LS: HISAT: A fast spliced aligner with low memory requirements. Nat Methods. 12:357–360. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL and Pachter L: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 7:562–578. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Anders S and Huber W: Differential expression analysis for sequence count data. Genome Biol. 11:R1062010. View Article : Google Scholar : PubMed/NCBI

27 

Gao X, Wang J and Zhang S: Integrated bioinformatics analysis of Hub genes and pathways in anaplastic thyroid carcinomas. Int J Endocrinol. 2019:96513802019. View Article : Google Scholar : PubMed/NCBI

28 

Kanehisa M and Goto S: KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28:27–30. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Robinson MD, McCarthy DJ and Smyth GK: edgeR: A Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 26:139–140. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Martineau E, Khantache KE, Pupier M, Sepulcric P, Akoka S and Giraudeau P: Non-linear effects in quantitative 2D NMR of polysaccharides: Pitfalls and how to avoid them. J Pharm Biomed Anal. 108:78–85. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Jahanbin K, Abbasian A and Ahang M: Isolation, purification and structural characterization of a new water-soluble polysaccharide from Eremurus stenophyllus (boiss. & buhse) baker roots. Carbohydrate Polymers. 178:386–393. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Santos G, Oliveira ES, Pinheiro ADN, da Costa PM, de Freitas JCC, de Araújo Santos FG, Maia FMM, de Morais SM and Nunes-Pinheiro DCS: Himatanthus drasticus (Apocynaceae) latex reduces oxidative stress and modulates CD4+, CD8+, FoxP3+ and HSP-60+ expressions in Sarcoma 180-bearing mice. J Ethnopharmacol. 220:159–168. 2018. View Article : Google Scholar : PubMed/NCBI

33 

David B, Edward H, Nick A, Takeshi K, Udo O, Simon H, Daniel S, Dietrich L, John A and Hassan AB: A germline mutation of CDKN2A and a novel RPLP1-C19MC fusion detected in a rare melanotic neuroectodermal tumor of infancy: A case report. BMC Cancer. 16:629–639. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Derenzini M, Montanaro L and Trerè D: Ribosome biogenesis and cancer. Acta Histochem. 119:190–197. 2017. View Article : Google Scholar : PubMed/NCBI

35 

Michael GK, Jill AI, Smrithi MP, Alex SC and Vassie CW: RpL22e, but not RpL22e-like-PA, is SUMOylated and localizes to the nucleoplasm of Drosophila meiotic spermatocytes. Nucleus. 4:241–258. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Kirita T and Wanaka A: The role of the Wnt signaling pathway in upper jaw development of chick embryo. Acta Histochem Cytochem. 52:19–26. 2019. View Article : Google Scholar : PubMed/NCBI

37 

Young BK, Boh-Ram K, Kyungsil Y, Choi EK, Seo SH, Yeonah L, Min AL, Jung BY, Mi SP and Seung BR: WIF1 can effectively co-regulate pro-apoptotic activity through the combination with DKK1. Cell Signal. 26:2562–2572. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Li C, Wang Z, Chen S, Zhang J, Qu K and Liu C: MicroRNA-552 promotes hepatocellular carcinoma progression by downregulating WIF1. Int J Mol Med. 42:3309–3317. 2018.PubMed/NCBI

39 

Kojima T, Shimazui T, Hinotsu S, Joraku A, Oikawa T, Kawai K, Horie R, Suzuki H, Nagashima R, Yoshikawa K, et al: Decreased expression of CXXC4 promotes a malignant phenotype in renal cell carcinoma by activating Wnt signaling. Oncogene. 28:297–305. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Chiurillo M: Role of the Wnt/β-catenin pathway in gastric cancer: An in-depth literature review. World J Exp Med. 5:84–102. 2015. View Article : Google Scholar : PubMed/NCBI

41 

Hino S, Kishida S, Michiue T, Fukui A, Sakamoto I, Takada S, Asashima M and Kikuchi A: Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein. Mol Cell Biol. 21:330–342. 2001. View Article : Google Scholar : PubMed/NCBI

42 

Wissmann C, Wild PJ, Kaiser S, Roepcke S, Stoehr R, Woenckhaus M, Kristiansen G, Hsieh JC, Hofstaedter F, Hartmann A, et al: WIF1, a component of the Wnt pathway, is down-regulated in prostate, breast, lung, and bladder cancer. J Pathol. 201:204–212. 2003. View Article : Google Scholar : PubMed/NCBI

43 

White JJ, Mazzeu JF, Coban-Akdemir Z, Bayram Y, Bahrambeigi V, Hoischen A, van Bon BWM, Gezdirici A, Gulec EY, Ramond F, et al: WNT signaling perturbations underlie the genetic heterogeneity of Robinow syndrome. Am J Hum Genet. 102:27–43. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Zhang T, Chen H, Zhou Y, Dong W, Cai H and Tan W: Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions. Bioresources Bioprocessing. 6:122019. View Article : Google Scholar

45 

Dolphin AC: Calcium channel auxiliary α-2/δ and β subunits: Trafficking and one step beyond. Nat Rev Neurosci. 13:542–555. 2012. View Article : Google Scholar : PubMed/NCBI

46 

Fernando C, Victoria S, Lorena A, Fredrik W, Erik S, Christopher JM and Piero C: RasGRF suppresses Cdc42-mediated tumour cell movement, cytoskeletal dynamics and transformation. Nat Cell Bio. 13:819–826. 2011. View Article : Google Scholar

47 

Elena S, David M, Michela S, Romilde M, Maria S, Rita G, Andrea M, Silvio T, Lilia A and Marco V: Novel RasGRF1-derived Tat-fused peptides inhibiting Ras-dependent proliferation and migration in mouse and human cancer cells. Biotechnol Adv. 30:233–243. 2012. View Article : Google Scholar : PubMed/NCBI

48 

James DC, Lin LL, Ronald WK and Clark JD: A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAP. Cell. 65:1043–1051. 1991. View Article : Google Scholar : PubMed/NCBI

49 

Faure G, Corringer PJ and Edelman A: Therapeutic potential of rattlesnake PLA2: Impact in Cystic Fibrosis. Toxicon. 149:93–94. 2018. View Article : Google Scholar

50 

Jaworska J and Janowski T: Expression of proinflammatory cytokines IL-1β, IL-6 and TNFα in the retained placenta of mares. Theriogenology. 126:1–7. 2018. View Article : Google Scholar : PubMed/NCBI

51 

Rostami H and Gharibzahedi S: Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities. Int J Biol Macromol. 101:196–206. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dong M, Hou Y and Ding X: Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél. Oncol Lett 20: 2169-2182, 2020.
APA
Dong, M., Hou, Y., & Ding, X. (2020). Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél. Oncology Letters, 20, 2169-2182. https://doi.org/10.3892/ol.2020.11761
MLA
Dong, M., Hou, Y., Ding, X."Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél". Oncology Letters 20.3 (2020): 2169-2182.
Chicago
Dong, M., Hou, Y., Ding, X."Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél". Oncology Letters 20, no. 3 (2020): 2169-2182. https://doi.org/10.3892/ol.2020.11761
Copy and paste a formatted citation
x
Spandidos Publications style
Dong M, Hou Y and Ding X: Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél. Oncol Lett 20: 2169-2182, 2020.
APA
Dong, M., Hou, Y., & Ding, X. (2020). Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél. Oncology Letters, 20, 2169-2182. https://doi.org/10.3892/ol.2020.11761
MLA
Dong, M., Hou, Y., Ding, X."Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél". Oncology Letters 20.3 (2020): 2169-2182.
Chicago
Dong, M., Hou, Y., Ding, X."Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél". Oncology Letters 20, no. 3 (2020): 2169-2182. https://doi.org/10.3892/ol.2020.11761
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