Open Access

Downregulation of microRNA‑126 is inversely correlated with insulin receptor substrate‑1 protein expression in colorectal cancer and is associated with advanced stages of disease

  • Authors:
    • Shicai Ye
    • Caiyuan Yu
    • Guixia Zhang
    • Feixiong Shi
    • Yongze  Chen
    • Jianyun Yang
    • Weiyun Wu
    • Yu Zhou
  • View Affiliations

  • Published online on: July 1, 2020     https://doi.org/10.3892/ol.2020.11796
  • Pages: 2411-2419
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is a common human malignant tumor, and the fourth most common cause of cancer‑associated mortality in China. However, the pathogenesis of CRC is not yet fully understood. The present study aimed to investigate the expression and clinical significance of microRNA (miR)‑126 and insulin receptor substrate‑1 (IRS‑1), as well as the role of miR‑126 in the prognosis of patients with CRC. A total of 86 colorectal tissue specimens, including 40 CRC and adjacent normal tissue, 26 colorectal adenoma tissue and 20 normal colorectal tissue samples, were collected for the present study. Reverse transcription‑quantitative PCR analysis was performed to determine miR‑126 and IRS‑1 mRNA expression levels, while western blotting and immunohistochemistry (IHC) analyses were performed to determine IRS‑1 protein expression levels. The correlation between miR‑126 and IRS‑1 expression, as well as the association between altered miR‑126 and IRS‑1 expression levels and clinicopathological characteristics, and the overall survival time of patients with CRC were assessed. The results demonstrated that miR‑126 expression was significantly downregulated, while IRS‑1 protein expression was upregulated in CRC tissues compared with that in adjacent normal tissues, colorectal adenoma tissues and normal colorectal tissues, respectively. IHC analysis exhibited strong positive staining of IRS‑1 protein in CRC tissues, while absent or weak staining of IRS‑1 protein was detected in adjacent normal tissues, colorectal adenoma tissues and normal colorectal tissues. miR‑126 expression was inversely correlated with IRS‑1 protein expression in CRC tissues (r=‑0.420; P<0.05). Furthermore, downregulated miR‑126 expression was associated with advanced clinicopathological characteristics of the disease and a shorter overall survival time in patients with CRC. Taken together, the results of the present study suggest that miR‑126 downregulation may be a candidate molecular marker predictive of poor prognosis of patients with CRC.

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September-2020
Volume 20 Issue 3

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Spandidos Publications style
Ye S, Yu C, Zhang G, Shi F, Chen Y, Yang J, Wu W and Zhou Y: Downregulation of microRNA‑126 is inversely correlated with insulin receptor substrate‑1 protein expression in colorectal cancer and is associated with advanced stages of disease. Oncol Lett 20: 2411-2419, 2020
APA
Ye, S., Yu, C., Zhang, G., Shi, F., Chen, Y., Yang, J. ... Zhou, Y. (2020). Downregulation of microRNA‑126 is inversely correlated with insulin receptor substrate‑1 protein expression in colorectal cancer and is associated with advanced stages of disease. Oncology Letters, 20, 2411-2419. https://doi.org/10.3892/ol.2020.11796
MLA
Ye, S., Yu, C., Zhang, G., Shi, F., Chen, Y., Yang, J., Wu, W., Zhou, Y."Downregulation of microRNA‑126 is inversely correlated with insulin receptor substrate‑1 protein expression in colorectal cancer and is associated with advanced stages of disease". Oncology Letters 20.3 (2020): 2411-2419.
Chicago
Ye, S., Yu, C., Zhang, G., Shi, F., Chen, Y., Yang, J., Wu, W., Zhou, Y."Downregulation of microRNA‑126 is inversely correlated with insulin receptor substrate‑1 protein expression in colorectal cancer and is associated with advanced stages of disease". Oncology Letters 20, no. 3 (2020): 2411-2419. https://doi.org/10.3892/ol.2020.11796