Open Access

Small RNA profiles of HTLV‑1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T‑cell antigen receptor γ‑chain using massively parallel sequencing: A pilot study

  • Authors:
    • Daniela Raguer Valadão De Souza
    • Rodrigo Pessôa
    • Andrezza Nacimento
    • Youko Nukui
    • Juliana Pereira
    • Jorge Casseb
    • Augusto César Penalva De Oliveira
    • Alberto José Da Silva Duarte
    • Patricia Bianca Clissa
    • Sabri Saeed Sanabani
  • View Affiliations

  • Published online on: July 1, 2020     https://doi.org/10.3892/ol.2020.11803
  • Pages: 2311-2321
  • Copyright: © Valadão De Souza et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present pilot study, massively parallel sequencing (MPS) technology was used to investigate cellular small RNA (sRNA) levels in the peripheral blood mononuclear cells (PBMCs) of human T‑lymphotropic virus type I (HTLV‑I) infected asymptomatic carriers with monoclonal (ASM) and polyclonal (ASP) T cell receptor (TCR) γ gene. Blood samples from 15 HTLV‑I asymptomatic carriers (seven ASM and eight ASP) were tested for the clonal TCR‑γ gene and submitted for sRNA library construction together with blood samples of five healthy controls (HCs) using Illumina sequencing platform. The sRNA‑sequencing reads were aligned, annotated and profiled using various bioinformatics tools. Based on these results, possible markers were validated in the study samples by performing reverse transcription‑quantitative (RT‑q)PCR analysis. A total of 76 known sRNAs and 52 putative novel sRNAs were identified. Among them, 44 known and 34 potential novel sRNAs were differentially expressed in the ASM and ASP libraries compared with HCs. In addition, 10 known sRNAs were exclusively dysregulated in the ASM group and one (transfer RNA 65) was significantly upregulated in the ASP group. Homo sapiens (hsa) microRNA (miRNA/mir)‑23a‑3p, ‑28‑5p, hsa‑let‑7e‑5p and hsa‑mir‑28‑3p and ‑361‑5p were the most abundantly upregulated mature miRNAs and hsa‑mir‑363‑3p, ‑532‑5p, ‑106a‑5p, ‑25‑3p and ‑30e‑5p were significantly downregulated miRNAs (P<0.05) with a >2‑fold difference between the ASM and ASP groups compared with HCs. Based on these results, hsa‑mir‑23a‑3p and ‑363‑3p were selected for additional validation. However, the quantification of these two miRNAs using RT‑qPCR did not provide any significant differences. While the present study failed to identify predictive sRNA markers to distinguish between ASM and ASP, the MPS results revealed differential sRNA expression profiles in the PBMCs of HTLV‑1 asymptomatic carriers (ASM and ASP) compared with HCs.

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September-2020
Volume 20 Issue 3

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Spandidos Publications style
Valadão De Souza DR, Pessôa R, Nacimento A, Nukui Y, Pereira J, Casseb J, Penalva De Oliveira AC, Da Silva Duarte AJ, Clissa PB, Sanabani SS, Sanabani SS, et al: Small RNA profiles of HTLV‑1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T‑cell antigen receptor γ‑chain using massively parallel sequencing: A pilot study. Oncol Lett 20: 2311-2321, 2020
APA
Valadão De Souza, D.R., Pessôa, R., Nacimento, A., Nukui, Y., Pereira, J., Casseb, J. ... Sanabani, S.S. (2020). Small RNA profiles of HTLV‑1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T‑cell antigen receptor γ‑chain using massively parallel sequencing: A pilot study. Oncology Letters, 20, 2311-2321. https://doi.org/10.3892/ol.2020.11803
MLA
Valadão De Souza, D. R., Pessôa, R., Nacimento, A., Nukui, Y., Pereira, J., Casseb, J., Penalva De Oliveira, A. C., Da Silva Duarte, A. J., Clissa, P. B., Sanabani, S. S."Small RNA profiles of HTLV‑1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T‑cell antigen receptor γ‑chain using massively parallel sequencing: A pilot study". Oncology Letters 20.3 (2020): 2311-2321.
Chicago
Valadão De Souza, D. R., Pessôa, R., Nacimento, A., Nukui, Y., Pereira, J., Casseb, J., Penalva De Oliveira, A. C., Da Silva Duarte, A. J., Clissa, P. B., Sanabani, S. S."Small RNA profiles of HTLV‑1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T‑cell antigen receptor γ‑chain using massively parallel sequencing: A pilot study". Oncology Letters 20, no. 3 (2020): 2311-2321. https://doi.org/10.3892/ol.2020.11803