Open Access

Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells

  • Authors:
    • Yong Lu
    • Shen-Kang Zhou
    • Rui Chen
    • Liang-Xian Jiang
    • Lei-Lei Yang
    • Tie-Nan Bi
  • View Affiliations

  • Published online on: September 3, 2020     https://doi.org/10.3892/ol.2020.12048
  • Article Number: 186
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. SAR1 gene homolog B (SAR1B) is a GTPase that has been reported to have a central role in the regulation of lipid homeostasis and is associated with numerous diseases. However, its role in cancer, particularly in CRC, remains unclear. The present study revealed that SAR1B was overexpressed in CRC samples and this was associated with shorter overall survival time in patients with CRC. Colony formation, cell proliferation and flow cytometry assays were conducted to evaluate the functions of SAR1B in CRC. It was reported that SAR1B may be associated with tumorigenesis of CRC. Knockdown of SAR1B suppressed cell proliferation and induced significant apoptosis of RKO cells. Furthermore, microarray analysis was performed to identify the potential targets of SAR1B in CRC. Bioinformatics analysis revealed that SAR1B was significantly involved in regulating ‘TGF‑β signaling’, ‘paxillin signaling’, ‘cell cycle regulation by BTG family proteins’ and ‘IGF‑1 signaling’. These results suggested that SAR1B may be considered a potential prognostic biomarker and therapeutic target for CRC.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Lu Y, Zhou S, Chen R, Jiang L, Yang L and Bi T: Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells. Oncol Lett 20: 186, 2020
APA
Lu, Y., Zhou, S., Chen, R., Jiang, L., Yang, L., & Bi, T. (2020). Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells. Oncology Letters, 20, 186. https://doi.org/10.3892/ol.2020.12048
MLA
Lu, Y., Zhou, S., Chen, R., Jiang, L., Yang, L., Bi, T."Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells". Oncology Letters 20.5 (2020): 186.
Chicago
Lu, Y., Zhou, S., Chen, R., Jiang, L., Yang, L., Bi, T."Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells". Oncology Letters 20, no. 5 (2020): 186. https://doi.org/10.3892/ol.2020.12048