RRBP1 is highly expressed in bladder cancer and is associated with migration and invasion

Wang,Mingshuai; Liu,Sai; Zhou,Bolin; Wang,Jianwen; Ping,Hao; Xing,Nianzeng

doi:10.3892/ol.2020.12066

RRBP1 is highly expressed in bladder cancer and is associated with migration and invasion

Authors:
- Mingshuai Wang
- Sai Liu
- Bolin Zhou
- Jianwen Wang
- Hao Ping
- Nianzeng Xing
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Affiliations: Department of Urology, Institute of Urology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China, Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Urology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China
Published online on: September 8, 2020 https://doi.org/10.3892/ol.2020.12066
Article Number: 203
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ribosome‑binding protein 1 (RRBP1) is a marker for colorectal, lung, esophageal and prostate cancer. However, the association between RRBP1 and bladder cancer is not completely understood. The present study aimed to evaluate the expression and function of RRBP1 in bladder cancer. The association between RRBP1 expression and clinicopathological characteristics, as well as the prognosis of bladder cancer was analyzed. RRBP1 expression was further analyzed in bladder cancer cell lines via reverse transcription‑quantitative PCR and western blotting. RRBP1 knockdown was established using short hairpin RNAs to investigate the function of RRBP1 in T24 cells. Compared with healthy bladder tissue, RRBP1 expression levels were significantly upregulated in bladder cancer tissue. High RRBP1 expression was associated with tumor stage, lymph node metastasis and shorter overall survival time. RRBP1 protein was highly expressed in bladder cancer cell lines compared with normal SV‑HUC‑1 cells. Compared with the control group, RRBP1 knockdown inhibited T24 migration and invasion by downregulating the expression of C‑C chemokine receptor type 7 (CCR7) protein. In conclusion, the present study indicated that RRBP1 was associated with bladder cancer migration, invasion and prognosis, and CCR7 might serve a role in the process.

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