MicroRNA‑150 suppresses p27<sup>Kip1</sup> expression and promotes cell proliferation in HeLa human cervical cancer cells

Oboshi,Wataru; Hayashi,Keisuke; Takeuchi,Hiroaki; Ikeda,Katsuhide; Yamaguchi,Yoshitaka; Kimura,Asako; Nakamura,Takehiro; Yukimasa,Nobuyasu

doi:10.3892/ol.2020.12073

November-2020 Volume 20 Issue 5

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November-2020 Volume 20 Issue 5

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Article Open Access

MicroRNA‑150 suppresses p27^Kip1 expression and promotes cell proliferation in HeLa human cervical cancer cells

Authors:
- Wataru Oboshi
- Keisuke Hayashi
- Hiroaki Takeuchi
- Katsuhide Ikeda
- Yoshitaka Yamaguchi
- Asako Kimura
- Takehiro Nakamura
- Nobuyasu Yukimasa
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Affiliations: Department of Medical Technology and Sciences, International University of Health and Welfare, Narita Chiba 286‑8686, Japan, Department of Clinical Laboratory, Shikoku Central Hospital, Shikokuchuo, Ehime 799‑0193, Japan, Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa 761‑0123, Japan

Copyright: © Oboshi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 210
|
Published online on: September 8, 2020

https://doi.org/10.3892/ol.2020.12073
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Abstract

MicroRNAs (miRNAs) exert critical roles in the majority of biological and pathological processes. Recent studies have associated miR‑150 with a number of different cancer types. However, little is known about miR‑150 targets in cervical cancer. In the present study, the HeLa human cervical cancer cell line was transfected with hsa‑miR‑150‑5p mimics, hsa‑miR‑150‑5p inhibitors or miRNA controls. miR‑150 was predicted to bind the 3'untranslated region (3'UTR) of the CDKN1B gene, which encodes the cyclin‑dependent kinase inhibitor 1B (p27^Kip1). The direct binding between miR‑150 and the 3'UTR of CDKN1B was confirmed using dual‑luciferase reporter assays. The effects of miR‑150 on CDKN1B mRNA expression, p27^Kip1 protein expression, cell cycle and cell proliferation were determined using reverse‑transcription quantitative PCR, western blot analysis, flow cytometry and WST‑8 assays, respectively. miR‑150 was demonstrated to directly target the 3'UTR of CDKN1B in transfected HeLa cells. The expression of CDKN1B mRNA and p27^Kip1 protein was reduced by miR‑150 mimics, and increased by miR‑150 inhibitors. Moreover, the overexpression of miR‑150 promoted cell cycle progression from the G0/G1 to the S phase and led to a significant increase in HeLa cell proliferation. The results of the present study indicated that miR‑150 promotes HeLa cell cycle progression and proliferation via the suppression of p27^Kip1 expression.

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