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Gemcitabine resistance in triple‑negative breast cancer cells can be reverted by Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol

  • Authors:
    • Yan Zhao
    • Haiyang Jiang
    • Ming Gu
    • Cong Zu
    • Xinyu Zheng
  • View Affiliations

  • Published online on: September 15, 2020     https://doi.org/10.3892/ol.2020.12109
  • Article Number: 247
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of drug resistance to chemotherapeutic agents has consistently presented a challenge in terms of the treatment of patients with triple‑negative breast cancer (TNBC). In the present study, gemcitabine (dFdC)‑resistant TNBC cells were established, and the effects of lentivirus‑deoxyribonucleoside kinase (dNK) and a mutated form of dNK (lentivirus‑dNKmut) on reversing the acquired drug resistance in dFdC‑resistant TNBC cells were explored. Quantitative PCR and western blotting experiment results suggested that Drosophila melanogaster (Dm)‑dNK was stably expressed in the lentivirus‑infected MDA‑MB‑231 and MDA-MB-231R cells in the nucleus or cytosol, and autoradiography experiments revealed similar levels of enzymatic activity in the cells expressing dNK or dNKmut. In vitro cytotoxicity assay revealed that the IC50 values of dFdC were decreased 30~50‑fold in the dFdC‑resistant MDA‑MB‑231 cells following lentiviral transfection with dNK or dNKmut, and this effect was associated with a significantly increased rate of apoptosis compared with the cells transfected with the negative control lentivirus. In conclusion, Dm‑dNK in the nucleus or cytosol may be a potential candidate for reversing acquired dFdC resistance in TNBC cells, which may form the basis of novel strategies for the treatment of patients with drug‑resistant TNBC.

Introduction

Triple-negative breast cancer (TNBC) accounts for 15–20% of all breast carcinomas and is associated with an aggressive disease progression and a high risk of relapse (1,2). The median survival time of relapsed patients with TNBC varies between 12 and 24 months (3). TNBC is not efficiently treated by the currently available therapeutic regimens; this may be attributed to the lack of estrogen, progesterone and Erb-B2 receptor tyrosine kinase 2 (ERBB2) receptors (4,5). Chemotherapy remains the primary systemic treatment, and the poor prognosis of TNBC is often ascribed to resistance to chemotherapeutic agents. After repeated cycles of chemotherapy, enhanced tumor resistance and severe side effects resulting from these agents worsen the clinical outcome, often leading to therapeutic failure (6).

In recent years, various lines of research have been performed that aimed to bypass drug resistance and improve the sensitivity to chemotherapeutic agents in cancer cells (7,8). The underlying mechanisms are often complicated, such as reducing the effective drug concentration in cells, establishing abnormalities in drug targets and altering the regulation of apoptosis (9). Various strategies, including RNA silencing, nanopreparations, co-administration of two or more strategies, novel cytotoxic agents and regulation of apoptosis, have been developed to overcome drug resistance in cancer cells (10,11).

Gemcitabine (2′,2′-difluorodeoxycytidine; dFdC) has been evaluated for its efficacy in the treatment of TNBC in a number of clinical trials and has been established as one of the most efficient chemotherapeutic drugs for various types of cancer in clinical practice (12,13). dFdC is taken up into the cells by human equilibrative nucleoside transporters and human concentrative nucleoside transporters (14,15). Once inside the cell, dFdC is phosphorylated by deoxycytidine kinase (dCK) to its monophosphorylated form, and subsequently by nucleotide kinases to its active metabolites, dFdC diphosphate (dFdCDP) and dFdC triphosphate (dFdCTP) (16). dFdCDP is an effective inhibitor of ribonucleotide diphosphate reductases including ribonucleoside-diphosphate reductase large subunit (RRM1), and resistance to dFdC is associated with increased expression of ribonucleotide reductase (17).

The phosphorylation induced by dFdC is the main rate-limiting step of the anticancer effect of dFdC (18); on the other hand, dFdC can be deactivated to its main metabolite 2′,2′-difluorodeoxyuridine (dFdU) by cytidine deaminase (CDA) (19). The cytotoxicity of dFdC is mainly associated with the cellular accumulation of dFdCTP (16). dFdCTP is incorporated into DNA, causing masked chain termination by inducing a G0/G1 and S-phase arrest in the cell cycle, which triggers apoptosis (20). In addition, dFdC decreases cellular deoxynucleotide (dNTP) pools and competes with them for incorporation into DNA, which, coupled with a decreased feedback inhibition of dCK, leads to an enhanced incorporation of dFdC into DNA (18).

Various therapeutic approaches have been proposed to overcome drug resistance induced by nucleoside kinase deficiency (2123). Previously published work from our laboratory has demonstrated that a multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster (Dm-dNK) may be a potential candidate suicide gene, and its effectiveness has been investigated in a number of tumor cell lines, including MDA-MB-231 (24), using viral systems (retrovirus-, adenovirus- and lentivirus-based vectors) combined with prodrugs such as araT, araC, gemcitabine and bromovinyldeoxyuridine (BVDU) (2531). The effectiveness of Dm-dNK has been demonstrated to be due to its broad substrate specificity regarding both purine and pyrimidine nucleoside analog phosphorylation and a higher catalytic rate compared with that of previously studied nucleoside kinases (3234).

Previous studies have demonstrated that dCK-deficient cell lines display a dFdC-resistant phenotype (35,36). Therefore, the hypothesis of the present study was that transfection with Dm-dNK may reverse the resistance to dFdC in TNBC cells. The present study aimed to develop a dFdC-resistant breast cancer MDA-MB-231 cell model to explore whether Dm-dNK may reverse dFdC resistance in TNBC and to determine the underlying mechanisms.

Materials and methods

Lentiviral packaging and titration

The basic plasmid was established has been previously described (31,37). Briefly, a three-plasmid system and lentiviral vectors were co-transfected into the packaging cells (the 293 cell line; 5×106 cells/100-mm dish cultured for 24 h until the cell confluence rate was 70–80%) through standard transient transfection using Lipofectamine® 2000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The rate of plasmid:vector:cells was 1:1:1. The medium was collected 48 h post-transfection and filtered through a 0.45-µm filter and diluted 2-fold with fresh medium, and then repeatedly collected three times at 24-h intervals. Subsequently, PCR was used to amplify the DNA fragment of Dm-dNK-3Flag and Dm-dNKmut-3Flag, and the fragment was then ligated into a PGC-FU plasmid (Shanghai GeneChem Co., Ltd.), which was composed of a 5′-long terminal repeat, a cytomegalovirus promoter and a multiple cloning site in the presence of a green fluorescent protein (GFP) sequence. Virus-producing cells were collected by ultracentrifugation (4°C, 120,000 × g for 2 h) to collect the recombinant lentivirus, followed by PCR identification. These lentiviruses were termed Lv-dNK and Lv-dNKmut. Lentiviral infectivity among cell lines was determined by dNK-GFP and dNKmut-GFP. To improve the infection efficiency, all cell lines were transduced (37°C for 24 h repeated three times) with lentivirus in DMEM supplemented with 6 µg/ml polybrene (Merck KGaA). The virus titer was quantified based on the number of GFP-positive cells and the infectious dose of the recombinant virus with 10-fold serial dilution. The cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). GFP and DAPI fluorescence was observed using a Nikon Eclipse E600 microscope (Nikon Corporation) equipped with a SPOT RT digital camera (Diagnostic Instruments, Inc.) at ×400 magnification in 5–6 fields per sample.

Cell culture and establishment of the dFdC-resistant cell line

293 and MDA-MB-231 cells were obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences. HEK293 and MDA-MB-231 cells were maintained in high-glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin at 37°C in a humidified atmosphere containing 5% CO2. The modified resistant strain of MDA-MB-231 was developed as previously described (38). Briefly, MDA-MB-231 cells were maintained in DMEM and exposed to dFdC at an initial concentration of 1.0 µM and repeatedly cultured with increasing concentrations of dFdC at 1.5–2-fold increments including dFdC-free intervals in order to allow the surviving cells to recover. MDA-MB-231 cells were seeded at a density of 3×103 cells/100-mm dish and subcultured every 14 days with increasing dosage of dFdC treatment from day 7 to day 12. This protocol was repeated twice. After 28 days, the cells were seeded at a density of 6×105 cells/100-mm dish and treated with dFdC after 24 h. After another 72 h, the cells were subcultured and cultured in dFdC-free medium for 96 h to allow the surviving cells to recover. This protocol was repeated five times. The final concentration was 80 µM. Chemoresistant MDA-MB-231 cells were challenged with dFdC for 6 months. Prior to the experiments, chemoresistant MDA-MB-231 cells were seeded into to the drug-free medium for 2 weeks, and these cells were termed MDA-MB-231R cells.

Western blotting analysis

MDA-MB-231 and MDA-MB-231R cells were harvested at 72 h following lentiviral infection, and total proteins were extracted using a lysis buffer [50 mM HEPES (pH 7.4), 250 mM NaCl, 1 mM NaF, 1 mM EDTA, 1% Triton X-100 and 1 mM DTT] supplemented with protease inhibitors PMSF (cat. no. 8553S; Cell Signaling Technology, Inc.). The concentration of the extracted protein was measured using a BCA protein assay (Nanjing KeyGen Biotech Co., Ltd.). Equal amounts (20 µg) of the proteins were subjected to SDS-PAGE (10% gels) and electro-transferred to PVDF membranes (EMD Millipore). The blots were blocked in Tris-buffered saline (TBS) containing 0.1% Tween-20 and 5% nonfat milk at room temperature for 2 h, followed by incubation with antibodies against Flag (1:1,000; cat. no. ab205606; Abcam), dCK (1:5,000; cat. no. ab151966; Abcam), CDA (1:300; cat. no. SAB1300717; Merck KGaA), P-gp (1:2,000; cat. no. ab170904; Abcam) and β-actin (1:500; cat. no. sc47778; Santa Cruz Biotechnology, Inc.) at 4°C overnight. Subsequently, the blots were washed with TBS containing 0.1% Tween-20, followed by incubation with an appropriate specific secondary antibody: Mouse anti-rabbit IgG-HRP (1:5,000; cat. no. sc2357; Santa Cruz Biotechnology, Inc.) or goat anti-mouse IgG-HRP (1:4,000; cat. no. sc2005; Santa Cruz Biotechnology, Inc.) at room temperature for 2 h. The immunoreactive bands were visualized with ECL western blotting substrate (Thermo Fisher Scientific, Inc.), and the protein expression was detected using the ChemiDoc™ XRS+ Imaging System (Bio-Rad Laboratories, Inc.). The relative band intensities were estimated using ImageJ 1.48 software (National Institutes of Health). β-actin was used as an internal loading control.

Reverse transcription-quantitative PCR (RT-qPCR)

Total RNA was extracted from the MDA-MB-231 and MDA-MB-231R cells using the AP-MN-MS-RNA-50 RNA extraction kit (Axygen; Corning, Inc.). RT was performed using a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's instructions. PCR amplification of the cDNA was performed in a 25 ml mixture containing 2 ml template cDNA, 1 ml each forward and reverse primer (10 µmol/l) and 12.5 ml SYBR® Premix Ex Taq II (Takara Biotechnology Co., Ltd.). GAPDH was used as an endogenous control. The primers used were as follows: Dm-dNK forward, 5′-ATGAGTTGCACGAGGACTGG-3′ and reverse, 5′-CTGGTACTCGGTGCCAATGT-3′; and GAPDH forward, 5′-ACAGTCCATGCCATCACTGCC-3′ and reverse, 5′-GCCTGCTTCACCACCTTCTTG-3′. The thermocycling conditions included an initial incubation at 95°C for 30 sec, followed by 40 cycles of denaturation at 95°C for 5 sec and annealing at 60°C for 30 sec. Each experiment was performed three times. The target gene levels were normalized to that of GAPDH as the housekeeping gene. Relative expression levels were calculated according to the 2−ΔΔCq method (39).

Enzyme activity assays

Cellular proteins were extracted using a previously established protocol (40) at 72 h post-infection with Lv-dNK or Lv-dNKmut at a multiplicity of infection (MOI) of 10. The activity of Dm-dNKmut was determined using a 35-ml reaction mixture comprising 50 mM Tris-HCl (pH 7.6), 100 mM KCl, 2 mM DTT, 15 mM NaF, 5 mM MgCl2, 5 mM ATP, 0.5 mg/ml bovine serum albumin (GBCBIO Technologies, Inc.) and 0.6 mg protein extract. For these analyses, aliquots of the samples, in which 2.5 mM [methyl-3H] deoxythymidine (dThd; Moravek, Inc.) was mixed with equivalent amounts of unlabeled substrates, were spotted onto Whatman DE-81 filter paper discs following incubation at 37°C for 10, 20 and 30 min. Subsequently, the paper discs were dried for 1 h, washed three times with 5 mM ammonium formate, the elution of nucleoside monophosphates was performed using 0.5 M KCl, and the radioactivity was subsequently determined using a scintillation counter.

Cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay

The viability of MDA-MB-231 and MDA-MB-231R cells was determined using the MTT assay. Briefly, cells were seeded in 96-well plates at a density of 104 cells/well and treated with graded concentrations of dFdC from 0.0001 to 100 µM for 48 h following infection with Lv-dNK or Lv-dNKmut. Subsequently, the medium was replaced with fresh medium (DMEM containing 10% FBS), and 20 µl MTT reagent (5 mg/ml; Promega Corporation) was added to each well, followed by incubation for 4 h. The formazan products were dissolved by 200 µl 99% dimethylsulfoxide (DMSO) for 10 min. The absorbance was examined by an enzyme immunoassay instrument at a wavelength of 570 nm. Each experiment was performed in triplicate.

Toxicity analysis with trypan blue dye exclusion assay

Cytotoxicity was evaluated using the trypan blue dye exclusion assay as previously described by Fraser et al (41). Each experiment was performed in a 35-mm tissue culture dish (three dishes per experimental condition) with 3×104 cells per dish with 1 ml high-glucose DMEM with 10% FBS was added. The plates were incubated overnight in the humidified incubator at 37°C to allow the cells to settle. The cells were subsequently infected with Dm-dNK, Dm-dNKmut or the empty lentiviral vector at an MOI of 1. Various concentrations of dFdC (0.01, 0.1, 1, 10 and 100 µM) were subsequently added, and the plates were incubated at 37°C for a further 24 h. Then, the medium was removed, followed by the addition of 0.25 ml trypan blue dye diluted in 0.8 ml medium. After 10-min incubation at 37°C in the dark, the diluted trypan blue was removed, and 30 fields of view with at least 20 cells in each field were captured under a microscope and counted using ImageJ 1.48 software (National Institutes of Health).

Apoptosis assay

Induction of apoptosis was analyzed by flow cytometry. Briefly, MDA-MB-231 and MDA-MB-231R cells were seeded in 6-well plates at 2×105 cells/well and cultured for 24 h, followed by transduction with lentiviral vectors, Lv-dNK or Lv-dNKmut at MOI of 10. Two days later, dFdC (1 µM) was added for 24 h, and the apoptosis assays were performed using an Annexin V-FITC kit (cat. no. KGA105; Nanjing Keygen Biotech Co., Ltd.) according to the manufacturer's instructions. The ratio of early and late apoptosis was assessed by a FACScan flow cytometer equipped with CELLQUEST and ModFITLT for Mac V1.01 software (Becton-Dickinson and Company).

Statistical analysis

Data are presented as the means ± SD. Data were analyzed using SPSS 23.0 (IBM Corp.). Differences between two groups were evaluated using unpaired Student's t-test, whereas one- and two-way ANOVA with Bonferroni post hoc test was performed for multiple comparisons. P<0.05 was considered to indicate a statistically significant value.

Results

Establishment of the drug-resistant breast cancer cell line

Parental cells MDA-MB-231 were continuously challenged with dFdC for 6 months to generate dFdC-resistant clones. As presented in Fig. 1A, the MDA-MB-231R cells were less sensitive to dFdC compared with the MDA-MB-231 cells. The mean IC50 values of MDA-MB-231R and MDA-MB-231 were 53.72 and 4.077 µM, respectively (P<0.001), exhibiting an ~13-fold increase in the MDA-MB-231R cells compared with the parental MDA-MB-231 cells. Subsequently, a concentration of 1 µM was selected for further experiments. Fig. 1B demonstrates that the cell viability was significantly inhibited in the MDA-MB-231R cells compared with that in the MDA-MB-231 cells after 48-h treatment with dFdC. Furthermore, the difference in cell proliferation between the two cell types increased over time.

The results also demonstrated that the levels of P glycoprotein (P-gp) were increased in MDA-MB-231R cells compared with those in the parental MDA-MB-231 cells (Fig. 1C). Subsequently, the protein expression levels of dCK and CDA were evaluated and were demonstrated to be associated with the dFdC resistance of MDA-MB-231R cells; the protein expression level of dCK was decreased, whereas that of CDA increased in the MDA-MB-231R cells compared with their parental cells (Fig. 1C and D), which was consistent with the results of previous studies (23,42), suggesting that the dFdC-resistant cell line was successfully established.

Assessment of the enzyme activity of Dm-dNK in the primary and drug-resistant breast cancer cell lines

In order to visualize the subcellular localization of the recombinant enzymes in vivo, the proteins were fused to GFP. MDA-MB-231 and MDA-MB-231R cells were transfected with the recombinant lentiviral vectors. Both cell lines were stably transfected, and green fluorescence was observed in 80–90% of the cells. Fluorescence in the nucleus was observed in cells transfected with the vector encoding dNK-GFP, whereas it was predominantly detected in the cytosol in cells transfected with the vector encoding dNKmut-GFP (Fig. S1). RT-qPCR and western blotting analysis demonstrated similar mRNA and protein expression levels of dNK-GFP and dNKmut-GFP in the MDA-MB-231 and MDA-MB-231R cells, irrespective of the subcellular localization (Fig. 2A-C).

In the present study, the levels of dThd phosphorylation in protein extracts of MDA-MB-231 and MDA-MB-231R cells were also evaluated to assess the enzymatic activity of the Dm-dNK proteins, as presented in Fig. 2D. The dThd kinase activity was increased 50-fold in MDA-MB-231 and MDA-MB-231R cells expressing nuclear Lv-dNK or cytosolic Lv-dNKmut compared with that in the cells transduced with lentiviral vectors. No significant differences in Dm-dNK activity were detected between the MDA-MB-231 and MDA-MB-231R cells.

Dm-dNK restores TNBC cell sensitivity to dFdC

The dFdC resistance of MDA-MB-231R cells was observed to be reversed following transfection with Lv-dNK or Lv-dNKmut at the MOI of 1 (Fig. 3A and B). The IC50 values for dFdC in MDA-MB-231R cells expressing Dm-dNK or dNKmut were 1.14 and 1.62 µM, respectively; The IC50 values for dFdC in MDA-MB-231 cells expressing dNK or dNKmut were 0.81 and 0.78 µM, respectively. These values were ~30-fold lower compared with those in the Lv group, irrespective of the protein localization (Fig. 3A and B), indicating that no differences in sensitivity to nucleoside analogs were observed between cells expressing nuclear and cytosolic Dm-dNK.

To gain further insights into the anticancer potential of Lv-dNK and Lv-dNKmut, apoptosis assay was performed in chemo-resistant MDA-MB-231R cells. As presented in Fig. 4A and B, following 24-h exposure to 1 µM dFdC, a significant increase in the apoptotic rate was observed in the MDA-MB-231R cells transfected with Lv-dNK and Lv-dNKmut compared with that in the control cells transduced with lentiviral vectors. The apoptotic rate in the control cells was 2–3%, whereas those in the Lv-dNK- and Lv-dNKmut-transfected cells were 19.7 and 14.2%, respectively. In addition, P-gp expression levels were partly decreased following transfection with Lv-dNK or Lv-dNKmut compared with the empty lentivirus group in MDA-MB-231R cells (Fig. 4C and D). These results revealed that the cell apoptotic rate of MDA-MB-231R cells was significantly increased due to active drug conversion from the prodrug.

Discussion

Resistance to anticancer drugs is a crucial problem that limits the effectiveness of chemotherapy regimens (43). Resistance generally develops with long-term exposure to the drug (44). In the present study, dFdC resistance was established in MDA-MB-231 cells through long-term treatment with dFdC, which was verified by MTT assay. Although the emergence of drug resistance has been associated with multiple molecular mechanisms, MDR1, which actively transports toxins out of the cells, has been strongly associated with the development of resistance to various chemotherapeutic agents (45). In the dFdC-resistant MDA-MB-231R cells established in the present study, expression of the MDR1-encoded P-gp was observed, but this was not the case for the MDA-MB-231 cells.

dCK is essential for the phosphorylation of dFdC, and CDA catalyzes the degradation of dFdC (18,19). dCK and CDA levels have been demonstrated to be significantly associated with dFdC sensitivity (46). It was reported that a high CDA-to-dCK ratio may be a marker of resistance to decitabine (an analog of cytidine) (47). Hosokawa et al (42) identified high protein expression levels of CDA and low levels of dCK in their successfully established HCT116 cells resistant to decitabine and dFdC. The protein expression levels of CDA and dCK were also examined in the present study, and similar results were obtained compared with those of a previous study (40), suggesting that the dFdC-resistant TNBC cells, which were termed MDA-MB-231R cells, were successfully established.

The expression of wild-type Dm-dNK in cell lines leads to nuclear localization of the enzyme, which can be attributed to the presence of a nuclear localization signal at the C-terminal region of the protein (40). The nuclear import of the protein is abolished by the site-directed mutation of arginine-247 to serine, leading to a predominant cytosolic localization of the enzyme (37,40). Our research team has previously investigated Dm-dNK for its potential application as a suicide gene; the results have revealed that wild-type Dm-dNK retains its activity when it is expressed in human cells, and it is localized to the nucleus, resulting in high cell sensitivity to several cytotoxic nucleoside analogs, including araT, araC, BVDU and dFdC (25,27,37,40). In the present study, either the wild-type nuclear Dm-dNK (dNK-GFP) or the cytosolic arginine-247 Dm-dNK mutant (dNKmut-GFP) was expressed using lentiviral vectors and the mutant cytosolic Dm-dNK was also demonstrated to possess highly similar levels of enzymatic activity and cytotoxicity compared with the wild-type dNK (33,37), consistent with the findings of the present study. As one of the most effective substrates for Dm-dNK, dFdC exerts strong effects on Dm-dNK-expressing MDA-MB-231R cells, which exhibit a 50-fold decrease in IC50 value for dFdC compared with that of untransfected cells (40), suggesting that the nucleoside analogs (prodrug)/Dm-dNK system may overcome drug resistance by lowering the IC50 values for these chemotherapeutic agents. As for the underlying mechanism, the apoptosis assay revealed that such an increase in sensitivity may be attributed to an enhanced rate of apoptosis. To the best of the authors' knowledge, the present study was the first study to have examined the effects of the suicide gene Dm-dNK and its mutant on reversing drug resistance in TNBC cells.

The present study had certain limitations. Cancer cells that acquire resistance to one anticancer drug may also become simultaneously resistant to different drugs, which has been referred to as multidrug-resistance or cross-resistance (42,48). Dm-dNK or Dm-dNKmut may also be able to reverse the drug-resistance that develops in cancer treatments with other chemotherapeutic agents, such as BVDU and araT, which was not investigated in the present study. In addition, the underlying mechanism of Dm-dNK reversing the drug resistance is still unclear and will be investigated in future studies.

In conclusion, Dm-dNK and Dm-dNKmut may be used to reverse the drug resistance encountered in cancer chemotherapy. This may form the basis for novel strategies in the treatment of patients with TNBC who have developed drug resistance.

Supplementary Material

Supporting Data

Acknowledgements

Not applicable.

Funding

This work was supported by grants from the Natural Science Foundation of Liaoning Province (grant no. 20180551215), Key R & D Plan Guidance Project of Liaoning Province (grant no. 2019JH8/10300020), and the Key Project of China Health Promotion Foundation (grant no. CHPF-RXO180301).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

YZ and XZ designed the study. YZ, MG and CZ performed the experiments. YZ and HJ collected and interpreted the data and performed the statistical analysis. YZ, HJ and XZ wrote and revised the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent to participate

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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November-2020
Volume 20 Issue 5

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Online ISSN:1792-1082

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Spandidos Publications style
Zhao Y, Jiang H, Gu M, Zu C and Zheng X: Gemcitabine resistance in triple‑negative breast cancer cells can be reverted by <em>Drosophila melanogaster</em> deoxyribonucleoside kinase in the nucleus or cytosol. Oncol Lett 20: 247, 2020.
APA
Zhao, Y., Jiang, H., Gu, M., Zu, C., & Zheng, X. (2020). Gemcitabine resistance in triple‑negative breast cancer cells can be reverted by <em>Drosophila melanogaster</em> deoxyribonucleoside kinase in the nucleus or cytosol. Oncology Letters, 20, 247. https://doi.org/10.3892/ol.2020.12109
MLA
Zhao, Y., Jiang, H., Gu, M., Zu, C., Zheng, X."Gemcitabine resistance in triple‑negative breast cancer cells can be reverted by <em>Drosophila melanogaster</em> deoxyribonucleoside kinase in the nucleus or cytosol". Oncology Letters 20.5 (2020): 247.
Chicago
Zhao, Y., Jiang, H., Gu, M., Zu, C., Zheng, X."Gemcitabine resistance in triple‑negative breast cancer cells can be reverted by <em>Drosophila melanogaster</em> deoxyribonucleoside kinase in the nucleus or cytosol". Oncology Letters 20, no. 5 (2020): 247. https://doi.org/10.3892/ol.2020.12109