mRNA expression and DNA methylation analysis of the inhibitory mechanism of H<sub>2</sub>O<sub>2</sub> on the proliferation of A549&nbsp;cells

Li,Yepeng; Wei,Zhongheng; Huang,Shiqing; Yang,Bo

doi:10.3892/ol.2020.12151

mRNA expression and DNA methylation analysis of the inhibitory mechanism of H₂O₂ on the proliferation of A549 cells

Authors:
- Yepeng Li
- Zhongheng Wei
- Shiqing Huang
- Bo Yang
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Affiliations: Department of Oncology, Biomedical Research Center, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region 533000, P.R. China, Key Laboratory of Guangxi College and Universities, Biomedical Research Center, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region 533000, P.R. China
Published online on: September 23, 2020 https://doi.org/10.3892/ol.2020.12151
Article Number: 288
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Reactive oxygen species, particularly hydrogen peroxide (H₂O₂), can induce proliferation inhibition and death of A549 cells via oxidative stress. Oxidative stress has effect on DNA methylation. Oxidative stress and DNA methylation feature a common denominator: The one carbon cycle. To explore the inhibitory mechanism of H₂O₂ on the proliferation of lung cancer cells, the present study analysed the mRNA expression and methylation profiles in A549 cells treated with H₂O₂ for 24 h, as adenocarcinoma is the most common pathological type of lung cancer. The DNA methylation profile was constructed using reduced representation bisulphite sequencing, which identified 29,755 differentially methylated sites (15,365 upregulated and 14,390 downregulated), and 1,575 differentially methylated regions located in the gene promoters were identified using the methylKit. Analysis of the assocaition between gene expression and methylation levels revealed that several genes were downregulated and hypermethylated, including cyclin‑dependent kinase inhibitor 3, denticleless E3 ubiquitin protein ligase homolog, centromere protein (CENP)F, kinesin family member (KIF)20A, CENPA, KIF11, PCNA clamp‑associated factor and GINS complex subunit 2, which may be involved in the inhibitory process of H2O2 on the proliferation of A549 cells.

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