Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma

Li,Hong-Tao; Wei,Bing; Li,Zhou-Quan; Wang,Xiao; Jia,Wen-Xian; Xu,Yan-Zhen; Liu,Jia-Yi; Shao,Meng-Nan; Chen,Sui-Xia; Mo,Nan-Fang; Zhao,Dong; Zuo,Wen-Pu; Qin,Jian; Li,Ping; Zhang,Qin-Le; Yang,Xiao-Li

doi:10.3892/ol.2020.12171

Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma

Authors:
- Hong-Tao Li
- Bing Wei
- Zhou-Quan Li
- Xiao Wang
- Wen-Xian Jia
- Yan-Zhen Xu
- Jia-Yi Liu
- Meng-Nan Shao
- Sui-Xia Chen
- Nan-Fang Mo
- Dong Zhao
- Wen-Pu Zuo
- Jian Qin
- Ping Li
- Qin-Le Zhang
- Xiao-Li Yang
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Affiliations: Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China, College of International Education, Guilin Medical University, Guilin, Guangxi 541100, P.R. China, College of Pharmacy, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Pathology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Genetic and Metabolic Central Laboratory, The Maternal and Children Health Hospital of Guangxi, Nanning, Guangxi 530005, P.R. China
Published online on: September 30, 2020 https://doi.org/10.3892/ol.2020.12171
Article Number: 308
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant DNA replication is one of the driving forces behind oncogenesis. Furthermore, minichromosome maintenance complex component 3 (MCM3) serves an essential role in DNA replication. Therefore, in the present study, the diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma (HCC) were investigated. By utilizing The Cancer Genome Atlas (TCGA) database, global MCM3 mRNA levels were assessed in HCC and normal liver tissues. Its effects were further analyzed by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting and immunohistochemistry in 78 paired HCC and adjacent tissues. Functional and pathway enrichment analyses were performed using the Search Tool for the Retrieval of Interacting Genes database. The expression levels of proteins that interact with MCM3 were also analyzed using the TCGA database and RT‑qPCR. Finally, algorithms combining receiver operating characteristic (ROC) curves were constructed using binary logistic regression using the TCGA results. Increased MCM3 mRNA expression with high α‑fetoprotein levels and advanced Edmondson‑Steiner grade were found to be characteristic of HCC. Survival analysis revealed that high MCM3 expression was associated with poor outcomes in patients with HCC. In addition, MCM3 protein expression was associated with increased tumor invasion in HCC tissues. MCM3 and its interacting proteins were found to be primarily involved in DNA replication, cell cycle and a number of binding processes. Algorithms combining ROCs of MCM3 and its interacting proteins were found to have improved HCC diagnosis ability compared with MCM3 and other individual diagnostic markers. In conclusion, MCM3 appears to be a promising diagnostic biomarker for HCC. Additionally, the present study provides a basis for the multi‑gene diagnosis of HCC using MCM3.

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