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Article Open Access

lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis

  • Authors:
    • Lijiang Yu
    • Lingli Huo
    • Xiaolin Shao
    • Jizhi Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, Department of Stomatology, Traditional Chinese Medicine Hospital of Shijingshan District, Beijing 100043, P.R. China, Department of Stomatology, Beijing Ditan Hospital, Capital Medical University, Beijing 100013, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 310
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    Published online on: September 30, 2020
       https://doi.org/10.3892/ol.2020.12173
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Abstract

Recently, previous studies have shown that long non‑coding RNA (lncRNA) can act as a tumor promoter or inhibitor in the pathogenesis of oral squamous cell carcinoma (OSCC). However, the regulatory mechanism of lncRNA SNHG5 is unknown in OSCC. Therefore, the functional mechanism of lncRNA SNHG5 in OSCC was initially revealed in this study. Here, RT‑qPCR and western blot analysis were used to assess mRNA and protein expression. The functional mechanism of SNHG5 was investigated by MTT, Transwell and luciferase reporter assays. The results showed that SNHG5 expression was upregulated in OSCC and promoted the viability, migration and invasion of OSCC cells. In addition, SNHG5 is the sponge of miR‑655‑3p in OSCC. And miR‑655‑3p was found to play an inhibitory effect in OSCC by interacting with SNHG5. Moreover, miR‑655‑3p directly targets FZD4 and negatively regulates its expression in OSCC. Functionally, FZD4 promoted the progression of OSCC by interacting with the SNHG5/miR‑655‑3p axis. In conclusion, lncRNA SNHG5 promotes cell proliferation, migration and invasion in OSCC by regulating miR‑655‑3p/FZD4 axis.
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1 

Chi AC, Day TA and Neville BW: Oral cavity and oropharyngeal squamous cell carcinoma-an update. CA Cancer J Clin. 65:401–421. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Zhang SK, Zheng R, Chen Q, Zhang S, Sun X and Chen W: Oral cancer incidence and mortality in China, 2011. Chin J Cancer Res. 27:44–51. 2015.PubMed/NCBI

3 

Brands MT, Brennan PA, Verbeek ALM, Merkx MAW and Geurts SME: Follow-up after curative treatment for oral squamous cell carcinoma. A critical appraisal of the guidelines and a review of the literature. Eur J Surg Oncol. 44:559–565. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Morra F, Merolla F, Picardi I, Russo D, Ilardi G, Varricchio S, Liotti F, Pacelli R, Palazzo L, Mascolo M, et al: CAF-1 subunits levels suggest combined treatments with PARP-inhibitors and ionizing radiation in advanced HNSCC. Cancers (Basel). 11:15822019. View Article : Google Scholar

5 

Sasahira T and Kirita T: Hallmarks of cancer-related newly prognostic factors of oral squamous cell carcinoma. Int J Mol Sci. 19:24132018. View Article : Google Scholar

6 

Bartonicek N, Maag JL and Dinger ME: Long noncoding RNAs in cancer: Mechanisms of action and technological advancements. Mol Cancer. 15:432016. View Article : Google Scholar : PubMed/NCBI

7 

Peng Z, Zhang C and Duan C: Functions and mechanisms of long noncoding RNAs in lung cancer. Onco Targets Ther. 9:4411–4424. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Jin Z, Jiang S, Jian S and Shang Z: Long noncoding RNA MORT overexpression inhibits cancer cell proliferation in oral squamous cell carcinoma by downregulating ROCK1. J Cell Biochem. Feb 25–2019.(Epub ahead of print). doi: 10.1002/jcb.28449. View Article : Google Scholar

9 

Yang Y, Chen D, Liu H and Yang K: Increased expression of lncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma. Cell Death Dis. 10:412019. View Article : Google Scholar : PubMed/NCBI

10 

Zhao L, Guo H, Zhou B, Feng J, Li Y, Han T, Liu L, Li L, Zhang S, Liu Y, et al: Long non-coding RNA SNHG5 suppresses gastric cancer progression by trapping MTA2 in the cytosol. Oncogene. 35:5770–5780. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Hu X, Hong Y and Shang C: Knockdown of long non-coding RNA SNHG5 inhibits malignant cellular phenotypes of glioma via Wnt/CTNNB1 signaling pathway. J Cancer. 10:1333–1340. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Zhang M, Li Q, Pan Y, Wang H, Liu G and Yin H: MicroRNA-655 attenuates the malignant biological behaviours of retinoblastoma cells by directly targeting PAX6 and suppressing the ERK and p38 MAPK signalling pathways. Oncol Rep. 39:2040–2050. 2018.PubMed/NCBI

13 

Kiuchi J, Komatsu S, Imamura T, Nishibeppu K, Shoda K, Arita T, Kosuga T, Konishi H, Shiozaki A, Okamoto K, et al: Low levels of tumour suppressor miR-655 in plasma contribute to lymphatic progression and poor outcomes in oesophageal squamous cell carcinoma. Mol Cancer. 18:22019. View Article : Google Scholar : PubMed/NCBI

14 

Gajera M, Desai N, Suzuki A, Li A, Zhang M, Jun G, Jia P, Zhao Z and Iwata J: MicroRNA-655-3p and microRNA-497-5p inhibit cell proliferation in cultured human lip cells through the regulation of genes related to human cleft lip. BMC Med Genomics. 12:702019. View Article : Google Scholar : PubMed/NCBI

15 

Wang W, Cao R, Su W, Li Y and Yan H: miR-655-3p inhibits cell migration and invasion by targeting pituitary tumor-transforming 1 in non-small cell lung cancer. Biosci Biotechnol Biochem. 83:1703–1708. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Lin J, Zandi R, Shao R, Gu J, Ye Y, Wang J, Zhao Y, Pertsemlidis A, Wistuba II, Wu X, et al: A miR-SNP biomarker linked to an increased lung cancer survival by miRNA-mediated down-regulation of FZD4 expression and Wnt signaling. Sci Rep. 7:90292017. View Article : Google Scholar : PubMed/NCBI

17 

Gupta S, Iljin K, Sara H, Mpindi JP, Mirtti T, Vainio P, Rantala J, Alanen K, Nees M and Kallioniemi O: FZD4 as a mediator of ERG oncogene-induced WNT signaling and epithelial-to-mesenchymal transition in human prostate cancer cells. Cancer Res. 70:6735–6745. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Chen L, Long Y, Han Z, Yuan Z, Liu W, Yang F, Li T, Shu L and Zhong Y: MicroRNA-101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Exp Ther Med. 17:1476–1485. 2019.PubMed/NCBI

19 

Wang Y, Zhang W, Wang Y and Wang S: HOXD-AS1 promotes cell proliferation, migration and invasion through miR-608/FZD4 axis in ovarian cancer. Am J Cancer Res. 8:170–182. 2018.PubMed/NCBI

20 

Tan J, Xiang L and Xu G: lncRNA MEG3 suppresses migration and promotes apoptosis by sponging miR-548d-3p to modulate JAK-STAT pathway in oral squamous cell carcinoma. IUBMB Life. 71:882–890. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Li B, Wang W, Miao S, Li G, Lv Y, Xiang C and Pei R: HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis. Cancer Cell Int. 19:1402019. View Article : Google Scholar : PubMed/NCBI

22 

Chi JR, Yu ZH, Liu BW, Zhang D, Ge J, Yu Y and Cao XC: SNHG5 promotes breast cancer proliferation by sponging the miR-154-5p/PCNA axis. Mol Ther Nucleic Acids. 17:138–149. 2019. View Article : Google Scholar : PubMed/NCBI

23 

Li X, Liu L, Luo Y, Cui S, Chen W, Zeng A, Shi Y and Luo L: Long non-coding RNA SNHG5 promotes glioma progression via miR-205/E2F3 axis. Biosci Rep. 39:BSR201906682019. View Article : Google Scholar : PubMed/NCBI

24 

Damas ND, Marcatti M, Come C, Christensen LL, Nielsen MM, Baumgartner R, Gylling HM, Maglieri G, Rundsten CF, Seemann SE, et al: SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization. Nat Commun. 7:138752016. View Article : Google Scholar : PubMed/NCBI

25 

Li Y, Guo D, Zhao Y, Ren M, Lu G, Wang Y, Zhang J, Mi C, He S and Lu X: Long non-coding RNA SNHG5 promotes human hepatocellular carcinoma progression by regulating miR-26a-5p/GSK3β signal pathway. Cell Death Dis. 9:8882018. View Article : Google Scholar : PubMed/NCBI

26 

Ju C, Zhou R, Sun J, Zhang F, Tang X, Chen KK, Zhao J, Lan X, Lin S, Zhang Z and Lv XB: lncRNA SNHG5 promotes the progression of osteosarcoma by sponging the miR-212-3p/SGK3 axis. Cancer Cell Int. 18:1412018. View Article : Google Scholar : PubMed/NCBI

27 

Gao J, Zeng K, Liu Y, Gao L and Liu L: lncRNA SNHG5 promotes growth and invasion in melanoma by regulating the miR-26a-5p/TRPC3 pathway. Onco Targets Ther. 12:169–179. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Lv ZD, Kong B, Liu XP, Jin LY, Dong Q, Li FN and Wang HB: miR-655 suppresses epithelial-to-mesenchymal transition by targeting Prrx1 in triple-negative breast cancer. J Cell Mol Med. 20:864–873. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Zha JF and Chen DX: miR-655-3p inhibited proliferation and migration of ovarian cancer cells by targeting RAB1A. Eur Rev Med Pharmacol Sci. 23:3627–3634. 2019.PubMed/NCBI

30 

Wang Y, Zang W, Du Y, Ma Y, Li M, Li P, Chen X, Wang T, Dong Z and Zhao G: Mir-655 up-regulation suppresses cell invasion by targeting pituitary tumor-transforming gene-1 in esophageal squamous cell carcinoma. J Transl Med. 11:3012013. View Article : Google Scholar : PubMed/NCBI

31 

Wu G, Zheng K, Xia S, Wang Y, Meng X, Qin X and Cheng Y: MicroRNA-655-3p functions as a tumor suppressor by regulating ADAM10 and β-catenin pathway in hepatocellular carcinoma. J Exp Clin Cancer Res. 35:892016. View Article : Google Scholar : PubMed/NCBI

32 

Harazono Y, Muramatsu T, Endo H, Uzawa N, Kawano T, Harada K, Inazawa J and Kozaki K: miR-655 is an EMT-suppressive microRNA targeting ZEB1 and TGFBR2. PLoS One. 8:e627572013. View Article : Google Scholar : PubMed/NCBI

33 

Yang Y, Sun Y, Wu Y, Tang D, Ding X, Xu W, Su B and Gao W: Downregulation of miR-3127-5p promotes epithelial-mesenchymal transition via FZD4 regulation of Wnt/β-catenin signaling in non-small-cell lung cancer. Mol Carcinog. 57:842–853. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Ma C, Xu B, Husaiyin S, Wang L, Wusainahong K, Ma J, Zhu K and Niyazi M: MicroRNA-505 predicts prognosis and acts as tumor inhibitor in cervical carcinoma with inverse association with FZD4. Biomed Pharmacother. 92:586–594. 2017. View Article : Google Scholar : PubMed/NCBI

35 

Yang J, Ye Z, Mei D, Gu H and Zhang J: Long noncoding RNA DLX6-AS1 promotes tumorigenesis by modulating miR-497-5p/FZD4/FZD6/Wnt/β-catenin pathway in pancreatic cancer. Cancer Manag Res. 11:4209–4221. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu L, Huo L, Shao X and Zhao J: lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis. Oncol Lett 20: 310, 2020.
APA
Yu, L., Huo, L., Shao, X., & Zhao, J. (2020). lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis. Oncology Letters, 20, 310. https://doi.org/10.3892/ol.2020.12173
MLA
Yu, L., Huo, L., Shao, X., Zhao, J."lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis". Oncology Letters 20.6 (2020): 310.
Chicago
Yu, L., Huo, L., Shao, X., Zhao, J."lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis". Oncology Letters 20, no. 6 (2020): 310. https://doi.org/10.3892/ol.2020.12173
Copy and paste a formatted citation
x
Spandidos Publications style
Yu L, Huo L, Shao X and Zhao J: lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis. Oncol Lett 20: 310, 2020.
APA
Yu, L., Huo, L., Shao, X., & Zhao, J. (2020). lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis. Oncology Letters, 20, 310. https://doi.org/10.3892/ol.2020.12173
MLA
Yu, L., Huo, L., Shao, X., Zhao, J."lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis". Oncology Letters 20.6 (2020): 310.
Chicago
Yu, L., Huo, L., Shao, X., Zhao, J."lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR‑655‑3p/FZD4 axis". Oncology Letters 20, no. 6 (2020): 310. https://doi.org/10.3892/ol.2020.12173
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