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Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells

  • Authors:
    • Xiaoyu Zhang
    • Tianyu Zhai
    • Zhenyu Hei
    • Di Zhou
    • Longyang Jin
    • Chao Han
    • Jiandong Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China, Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China, Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 311
    |
    Published online on: September 30, 2020
       https://doi.org/10.3892/ol.2020.12174
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Abstract

Platycodin D (PD) is a triterpenoid saponin that exists in the roots of Platycodonis. It exhibits evident growth inhibitory effects and potent cytotoxicity against multiple types of cancer. Gallbladder cancer (GBC) is the most common malignant disease of the biliary tract system. Patients with GBC usually have limited available treatment strategies and a poor prognosis. The present study investigated the antitumor effects of PD on human GBC cells in vitro and its underlying molecular mechanisms of action. The results indicated that PD, as assessed using MTT and colony forming assays, induced evident growth inhibition. Flow cytometry indicated that PD robustly induced apoptosis and blocked GBC cells at the G2/M phase. Cell migration and invasion assays demonstrated that PD effectively inhibited the migratory and invasive abilities of GBC cell lines. Western blotting indicated that PD may initiate mitochondrial destruction in GBC cells through the JNK signaling pathway, thereby inducing apoptosis. The present results indicated that PD may exhibit antitumor effects by inducing apoptosis; inhibiting migration and invasion; and affecting the cell cycle in GBC cells. Therefore, PD has the potential to become a novel antitumor drug for GBC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Zhai T, Hei Z, Zhou D, Jin L, Han C and Wang J: Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells. Oncol Lett 20: 311, 2020.
APA
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., & Wang, J. (2020). Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells. Oncology Letters, 20, 311. https://doi.org/10.3892/ol.2020.12174
MLA
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., Wang, J."Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells". Oncology Letters 20.6 (2020): 311.
Chicago
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., Wang, J."Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells". Oncology Letters 20, no. 6 (2020): 311. https://doi.org/10.3892/ol.2020.12174
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Zhai T, Hei Z, Zhou D, Jin L, Han C and Wang J: Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells. Oncol Lett 20: 311, 2020.
APA
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., & Wang, J. (2020). Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells. Oncology Letters, 20, 311. https://doi.org/10.3892/ol.2020.12174
MLA
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., Wang, J."Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells". Oncology Letters 20.6 (2020): 311.
Chicago
Zhang, X., Zhai, T., Hei, Z., Zhou, D., Jin, L., Han, C., Wang, J."Effects of Platycodin D on apoptosis, migration, invasion and cell cycle arrest of gallbladder cancer cells". Oncology Letters 20, no. 6 (2020): 311. https://doi.org/10.3892/ol.2020.12174
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