Open Access

Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs in vitro

  • Authors:
    • Lingli Xue
    • Yan Zeng
    • Chuan Fang
    • Wei Cheng
    • Yadong Li
  • View Affiliations

  • Published online on: October 8, 2020     https://doi.org/10.3892/ol.2020.12203
  • Article Number: 340
  • Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Nitrotyrosine, a structural analogue of tyrosine, is present in cells in pathological conditions and is incorporated into tubulin to form tubulin tyrosine nitration, which disrupts the normal function of microtubules. There is limited research on the functional aspects of tubulin tyrosine nitration in different types of tumor. In the present study, the effect of tubulin tyrosine nitration and tubulin tyrosine ligase like 12 (TTLL12) on the proliferation of SCC‑25 cells was investigated. TTLL12‑overexpressing cell lines were constructed and used to assess the effect of tubulin tyrosine nitration and TTLL12 on the proliferation of SCC‑25 cells via western blotting, immunofluorescent and MTT assays. An TTLL12‑stably overexpressing SCC‑25 cell line and the enzyme‑linked immunosorbent assay were used to establish a novel experiment in vitro for screening anticancer drugs targeting tubulin tyrosine nitration by assessing its sensitivity, specificity and repeatability, and using it to find an effective drug. The results demonstrated that the proliferative rate of the control cells was notably inhibited in the presence of nitrotyrosine compared with that of TTLL12‑overexpressing cells. The results of the MTT assay revealed that the proliferation of TTLL12‑silenced cells was significantly inhibited compared with that of the control group. The sensitivity, specificity and repeatability of the experiment were positive. It was found that nocodazole could have better anticancer effect than paclitaxel. Taken together, the results of the present study suggest that TTLL12 enhances SCC‑25 cell survival in the presence of nitrotyrosine by disrupting nitration of the tyrosine residues of tubulin, and tubulin tyrosine nitration may be developed for the basic research of anticancer drugs.
View Figures
View References

Related Articles

Journal Cover

December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xue L, Zeng Y, Fang C, Cheng W and Li Y: Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs<em> in&nbsp;vitro</em>. Oncol Lett 20: 340, 2020
APA
Xue, L., Zeng, Y., Fang, C., Cheng, W., & Li, Y. (2020). Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs<em> in&nbsp;vitro</em>. Oncology Letters, 20, 340. https://doi.org/10.3892/ol.2020.12203
MLA
Xue, L., Zeng, Y., Fang, C., Cheng, W., Li, Y."Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs<em> in&nbsp;vitro</em>". Oncology Letters 20.6 (2020): 340.
Chicago
Xue, L., Zeng, Y., Fang, C., Cheng, W., Li, Y."Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs<em> in&nbsp;vitro</em>". Oncology Letters 20, no. 6 (2020): 340. https://doi.org/10.3892/ol.2020.12203