MicroRNA‑155‑5p inhibits the invasion and migration of prostate cancer cells by targeting SPOCK1
- Lin-Ya Yao
- Jun Ma
- Xue-Ming Zeng
- Jun Ou‑yang
Affiliations: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Urology, Kunshan Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Kunshan, Jiangsu 215300, P.R. China
- Published online on: October 11, 2020 https://doi.org/10.3892/ol.2020.12215
Copyright: © Yao
et al. This is an open access article distributed under the
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The aim of the present study was to determine the effect of microRNA (miR)‑155‑5p on the expression of testican‑1 (SPOCK1) and the invasion and migration of prostate cancer cells in vitro. Bioinformatics analysis and molecular biology assays revealed that SPOCK1 may be a direct target gene of miR‑155‑5p. In addition, a negative correlation was identified between SPCOK1 and miR‑155‑5p expression in prostate tumor tissues and cell lines. miR‑155‑5p mimic transfection inhibited SPOCK1 expression in PC3 cells and decreased cell migration and invasion abilities, while the expression of vimentin, N‑cadherin, E‑cadherin, β‑catenin, matrix metalloproteinase (MMP)3 and MMP9 was upregulated. In summary, SPOCK1 was found to be a target gene of miR155‑5p in prostate cancer, and miR‑155‑5p acts as a tumor‑suppressor gene and may inhibit SPOCK1‑mediated prostate cancer progression.