Long non‑coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR‑331‑3p/RAB5B pathway

Li,Xiao-Mei; Jiao,Yan-Yan; Luan,Bao-Hong; Wu,Hong-Xia; Wang,Rong-Rong; Zhong,Jie

doi:10.3892/ol.2020.12219

Long non‑coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR‑331‑3p/RAB5B pathway

Authors:
- Xiao-Mei Li
- Yan-Yan Jiao
- Bao-Hong Luan
- Hong-Xia Wu
- Rong-Rong Wang
- Jie Zhong
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Affiliations: Department of Oncology, Qing Dao Cheng Yang People's Hospital, Qingdao, Shandong 266109, P.R. China, Department of Interventional Radiography, Qing Dao Cheng Yang People's Hospital, Qingdao, Shandong 266109, P.R. China
Published online on: October 13, 2020 https://doi.org/10.3892/ol.2020.12219
Article Number: 355
Copyright : © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Gastric cancer (GC) remains a threat to the health of the global population. The present study investigated the effects and mechanisms of the long non‑coding RNA myocardial infarction associated transcript (MIAT) on the proliferation, apoptosis and metastasis of GC (HGC‑27 and AGS) cells. The expression levels of MIAT, micoRNA (miR)‑331‑3p and RAB5B mRNA were analyzed using reverse transcription‑quantitative PCR analysis. Cell growth, apoptosis, migration and invasion were measured using 5‑ethynyl‑2'‑deoxyuridine, ﬂow cytometry, wound healing and Transwell assays, respectively. A luciferase assay was used to determine whether miR‑331‑3p targeted MIAT and RAB5B. The results indicated that MIAT levels were significantly upregulated in GC tissues and cells, correlated with RAB5B levels and inversely associated with miR‑331‑3p levels. MIAT overexpression promoted proliferation and metastasis, and inhibited the apoptosis of GC cells. MIAT knockdown had the opposite effect on GC cells. The rescue experiments revealed that the effects of MIAT knockdown on the biological behaviour of GC cells were attenuated by RAB5B overexpression. These data suggest that MIAT promotes GC progression via modulating miR‑331‑3p/RAB5B pathway.

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