Open Access

Genome‑wide methylation profiles in monozygotic twins with discordance for ovarian carcinoma

  • Authors:
    • Ozge Sukruoglu Erdogan
    • Seref Bugra Tuncer
    • Seda Kilic
    • Demet Akdeniz Odemis
    • Gozde Kuru Turkcan
    • Betul Celik
    • Mukaddes Avsar
    • Hulya Yazici
  • View Affiliations

  • Published online on: October 14, 2020     https://doi.org/10.3892/ol.2020.12221
  • Article Number: 357
  • Copyright: © Erdogan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Ovarian cancer is a disease that is generally diagnosed at an advanced stage, and has poor survival. Monozygotic (MZ) twins are considered to be good research models for investigating the epigenetic changes associated with diseases. In the present study, the involvement of epigenetic mechanisms in ovarian cancer etiology were evaluated using the MZ twin model. Whole‑genome methylation patterns were investigated in a BRCA1 gene mutation‑carrying family comprising MZ twins, only one of whom had ovarian cancer, and other healthy siblings. Whole‑genome methylation patterns were assessed in peripheral blood DNA using Infinium MethylationEPIC BeadChips on an Illumina iScan device. The hypermethylated and hypomethylated genes were detected between cases and controls in four different comparison groups in order to evaluate the differences in methylation levels according to cancer diagnosis and BRCA mutation status. The obtained results showed that the differential methylations in 12 different genes, namely PR/SET domain 6, cytochrome B5 reductase 4, ZNF714, OR52M1, SEMA4D, CHD1L, CAPZB, clustered mitochondria homolog, RB‑binding protein 7, chromatin repair factor, ankyrin repeat domain 23, RIB43A domain with coiled‑coils 1 and C6orf227, were associated with ovarian cancer. Biological functional analysis of the genes detected in the study using the PANTHER classification system revealed that they have roles in biological processes including ‘biologic adhesion’, ‘regulation’, ‘cellular components organization’, ‘biogenesis’, ‘immune system functioning’, ‘metabolic functioning’ and ‘localization’. Overall, the present study suggested that epigenetic differences, such as methylation status, could be used as a non‑invasive biological markers for the early diagnosis and follow‑up of ovarian cancer.
View Figures
View References

Related Articles

Journal Cover

December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

The Cancer Story
Copy and paste a formatted citation
x
Spandidos Publications style
Erdogan OS, Tuncer SB, Kilic S, Odemis DA, Turkcan GK, Celik B, Avsar M and Yazici H: Genome‑wide methylation profiles in monozygotic twins with discordance for ovarian carcinoma. Oncol Lett 20: 357, 2020
APA
Erdogan, O.S., Tuncer, S.B., Kilic, S., Odemis, D.A., Turkcan, G.K., Celik, B. ... Yazici, H. (2020). Genome‑wide methylation profiles in monozygotic twins with discordance for ovarian carcinoma. Oncology Letters, 20, 357. https://doi.org/10.3892/ol.2020.12221
MLA
Erdogan, O. S., Tuncer, S. B., Kilic, S., Odemis, D. A., Turkcan, G. K., Celik, B., Avsar, M., Yazici, H."Genome‑wide methylation profiles in monozygotic twins with discordance for ovarian carcinoma". Oncology Letters 20.6 (2020): 357.
Chicago
Erdogan, O. S., Tuncer, S. B., Kilic, S., Odemis, D. A., Turkcan, G. K., Celik, B., Avsar, M., Yazici, H."Genome‑wide methylation profiles in monozygotic twins with discordance for ovarian carcinoma". Oncology Letters 20, no. 6 (2020): 357. https://doi.org/10.3892/ol.2020.12221