Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis

Du,Qiajun; Han,Jie; Gao,Shan; Zhang,Shangdi; Pan,Yunyan

doi:10.3892/ol.2020.12251

Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis

Authors:
- Qiajun Du
- Jie Han
- Shan Gao
- Shangdi Zhang
- Yunyan Pan
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Affiliations: Department of Clinical Laboratory Center, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, P.R. China, Department of Endocrinology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730030, P.R. China
Published online on: October 29, 2020 https://doi.org/10.3892/ol.2020.12251
Article Number: 388
Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hypoxia facilitates the progression of numerous cancers. Circular RNAs (circRNA) have been revealed to be involved in the process of tumors mediated by hypoxia. However, the role and molecular mechanism of circular RNA hsa_circ_0008450 (circ_0008450) in hepatocellular cancer (HCC) under hypoxic conditions has been rarely reported. Expression levels of circ_0008450, microRNA(miR)‑431 and A‑kinase anchor protein 1 (AKAP1) were examined using reverse transcription‑quantitative PCR. Cell viability, apoptosis and glycolysis were assessed via Cell Counting Kit‑8, flow cytometry and glycolysis assays, respectively. The association between circ_0008450 or AKAP1 and miR‑431 was verified via dual‑luciferase reporter assays. Protein levels of AKAP1 were detected by western blotting. Effect of hsa_circ_0008450 on tumor growth in vivo was confirmed by xenograft assays. Circ_0008450 was upregulated in HCC tissues and hypoxia‑disposed HCC cells. Depletion of circ_0008450 suppressed tumor growth in vivo and reversed the repression of apoptosis and the acceleration of viability and glycolysis of HCC cells induced by hypoxia treatment in vitro. Notably, circ_0008450 regulated AKAP1 expression by sponging miR‑431. Furthermore, miR‑431 inhibition reversed the circ_0008450 silencing‑mediated effects on viability, apoptosis and glycolysis in hypoxia‑treated HCC cells. Additionally, AKAP1 enhancement abolished the effects of miR‑431 upregulation on the viability, apoptosis and glycolysis in hypoxia‑treated HCC cells. In conclusion, circ_0008450 repression mitigated the progression of HCC under hypoxia by downregulating AKAP1 via miR‑431, providing a potential target for HCC treatment.

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