Open Access

Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12

  • Authors:
    • Gang Wang
    • Kai Yang
  • View Affiliations

  • Published online on: October 29, 2020     https://doi.org/10.3892/ol.2020.12252
  • Article Number: 389
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is the third most common malignant type of tumor worldwide. Neurensin‑2 (NRSN2) is a small neuronal membrane protein associated with tumorigenesis. Therefore, the present study aimed to investigate the association between NRSN2 and CRC, and further examined the underlying mechanism of its effect on CRC metastasis. Human CRC SW620 cells were used to determine the biological functions of NRSN2 in CRC. Cell counting Kit‑8 (CCK8), colony formation, wound‑healing and transwell assays were performed to evaluate the role of NRSN2 on survival and metastasis of SW620 cells. The interaction between NRSN2 and SOX12 was determined via bioinformatics analysis and confirmed using immunoprecipitation. It was identified that NRSN2 was highly expressed in CRC cells and served a critical role in CRC cell survival compared with in healthy colon epithelial cells. Furthermore, NRSN2‑knockdown inhibited the proliferation, invasion and migration of SW620 cells, while NRSN2 overexpression promoted these cellular processes. Additionally, it was demonstrated that NRSN2 could recruit SOX12 in SW620 cells. NRSN2‑knockdown decreased SOX12 expression, while NRSN2 overexpression upregulated SOX12 expression. Overall, the present results suggested NRSN2 as a novel biomarker for CRC diagnosis and identified NRSN2 as a potential therapeutic target for CRC treatment.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang G and Wang G: Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12. Oncol Lett 20: 389, 2020
APA
Wang, G., & Wang, G. (2020). Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12. Oncology Letters, 20, 389. https://doi.org/10.3892/ol.2020.12252
MLA
Wang, G., Yang, K."Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12". Oncology Letters 20.6 (2020): 389.
Chicago
Wang, G., Yang, K."Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12". Oncology Letters 20, no. 6 (2020): 389. https://doi.org/10.3892/ol.2020.12252