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Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells

  • Authors:
    • Xiangru Li
    • Xiaoju Zhou
    • Ying Liu
    • Jingjing Fan
    • Hongjing  Huo
    • Jingjing Yao
    • Lin Wang
    • Ningning Ma
  • View Affiliations / Copyright

    Affiliations: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 44
    |
    Published online on: November 17, 2020
       https://doi.org/10.3892/ol.2020.12305
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Abstract

Metastasis is a primary contributor to the low survival rates of patients with cancer. Enhanced migration and invasion are two key features of the metastatic transformation of cancer cells. Furthermore, despite the fact that overexpression of the monocarboxylate transporter (MCT)1 and 4 proteins has been found to promote the migration or invasion of cancer cells, previous findings have not been conclusive and have even been contradictory. The majority of these previous studies have relied on the silencing or inhibition of MCT1/4 expression or function in highly metastatic cell lines. Silencing can be transient or incomplete, and inhibition can result in off‑target effects. Employing a different approach, the present study stably transfected human MCT1 and MCT4 into the non‑carcinogenic murine NCTC clone 929 (L929) cell line, which had undetectable endogenous MCT1 and MCT4 expression. It was observed that overexpression of MCT4, and not MCT1, promoted the migration and invasion of L929 cells. It was also found that overexpression of an inactive form of the MCT4 transporter with a single amino acid mutation failed to promote either migration or invasion, which suggested that MCT4 activity is required. Since an epidermal growth factor receptor (EGFR) inhibitor could reverse the effect of MCT4‑overexpression, it was concluded that MCT4‑overexpression exert its functions through modulating the EGF/EGFR pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Zhou X, Liu Y, Fan J, Huo H, Yao J, Wang L and Ma N: Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells. Oncol Lett 21: 44, 2021.
APA
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J. ... Ma, N. (2021). Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells. Oncology Letters, 21, 44. https://doi.org/10.3892/ol.2020.12305
MLA
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J., Wang, L., Ma, N."Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells". Oncology Letters 21.1 (2021): 44.
Chicago
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J., Wang, L., Ma, N."Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells". Oncology Letters 21, no. 1 (2021): 44. https://doi.org/10.3892/ol.2020.12305
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Zhou X, Liu Y, Fan J, Huo H, Yao J, Wang L and Ma N: Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells. Oncol Lett 21: 44, 2021.
APA
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J. ... Ma, N. (2021). Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells. Oncology Letters, 21, 44. https://doi.org/10.3892/ol.2020.12305
MLA
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J., Wang, L., Ma, N."Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells". Oncology Letters 21.1 (2021): 44.
Chicago
Li, X., Zhou, X., Liu, Y., Fan, J., Huo, H., Yao, J., Wang, L., Ma, N."Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non‑carcinogenic L929 fibroblast cells". Oncology Letters 21, no. 1 (2021): 44. https://doi.org/10.3892/ol.2020.12305
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