MicroRNA‑15a‑5p promotes the proliferation and invasion of T98G glioblastoma cells via targeting cell adhesion molecule 1

Kong,Fanqiang; Li,Xiaoqing; Li,Shuhong; Sheng,Dan; Li,Wenhu; Song,Mingming

doi:10.3892/ol.2020.12364

MicroRNA‑15a‑5p promotes the proliferation and invasion of T98G glioblastoma cells via targeting cell adhesion molecule 1

Authors:
- Fanqiang Kong
- Xiaoqing Li
- Shuhong Li
- Dan Sheng
- Wenhu Li
- Mingming Song
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Affiliations: Department of Emergency, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Obstetrics and Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
Published online on: December 10, 2020 https://doi.org/10.3892/ol.2020.12364
Article Number: 103
Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma (GBM) is a type of malignant tumor occurring in the brain that severely influences the life of affected individuals. GBM cells are highly infiltrative, which is one of the main obstacles in the treatment of the disease. Numerous microRNAs (miRNAs/miRs) are associated with the development of GBM. However, the effects of miR‑15a‑5p on GBM remain elusive. In the present study, reverse transcription‑quantitative PCR and western blot analysis were applied for the detection of RNA and protein levels, respectively. Cell Counting Kit‑8 and Transwell assays were performed to examine cell proliferation and invasion, respectively. TargetScan 7.1 and dual‑luciferase reporter assay were utilized for the prediction and verification of the association between miRNAs and mRNAs. The present study revealed that miR‑15a‑5p expression was upregulated in the GBM T98G cell line. The results further demonstrated that, through the inhibition of cell adhesion molecule 1 expression and the promotion of Akt phosphorylation, miR‑15a‑5p was able to promote GBM cell proliferation and invasion. Overall, the present findings revealed a novel mechanism responsible for the development of GBM and provided an experimental basis for the diagnosis and treatment of GBM.

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