Open Access

Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma

  • Authors:
    • Maciej Ciesielski
    • Mariusz Szajewski
    • Jakub Walczak
    • Rafał  Pęksa
    • Radosław  Lenckowski
    • Małgorzata Supeł
    • Jacek  Zieliński
    • Wiesław Janusz Kruszewski
  • View Affiliations

  • Published online on: December 21, 2020     https://doi.org/10.3892/ol.2020.12403
  • Article Number: 142
  • Copyright: © Ciesielski et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The accurate evaluation of human epidermal growth factor receptor 2 (HER2) status is essential for the appropriate use of targeted therapies. An increased number of chromosome 17 centromere enumeration probe (CEP17) signals may underrate fluorescence in situ hybridization (FISH) outcomes, resulting in false‑negative or a false‑equivocal HER2 status assessment. The aim of the present study was to assess the frequency of CEP17 copy number increase (CNI), its effects on HER2 protein expression (and the subsequent effects on tumor cells), and the survival outcomes of patients with gastric cancer. Archival primary tumor samples from 244 patients that underwent gastric resection for adenocarcinoma were retrieved for both HER2 protein expression analysis (using immunochemistry) and HER2 gene amplification (using FISH). The associations between HER2 status, CEP17 CNI and multiple clinicopathological parameters (including survival outcome), were assessed. The relationship between CEP17 CNI and HER2 protein upregulation was also investigated. CEP17 CNI was detected in 17.2% of cases, and a strong association between CEP17 CNI and HER2 upregulation was revealed. The impact of CEP17 CNI on survival did not reach statistical significance. Consequently, CEP17 CNI was discovered to be strongly associated with HER2 upregulation in tumor cells, which may characterize a critical issue in HER2 testing. Therefore, the eligibility for HER2‑targeted agents in CEP17 CNI‑positive patients warrants further recognition.
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February-2021
Volume 21 Issue 2

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Spandidos Publications style
Ciesielski M, Szajewski M, Walczak J, Pęksa R, Lenckowski R, Supeł M, Zieliński J and Kruszewski WJ: Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma. Oncol Lett 21: 142, 2021
APA
Ciesielski, M., Szajewski, M., Walczak, J., Pęksa, R., Lenckowski, R., Supeł, M. ... Kruszewski, W.J. (2021). Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma. Oncology Letters, 21, 142. https://doi.org/10.3892/ol.2020.12403
MLA
Ciesielski, M., Szajewski, M., Walczak, J., Pęksa, R., Lenckowski, R., Supeł, M., Zieliński, J., Kruszewski, W. J."Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma". Oncology Letters 21.2 (2021): 142.
Chicago
Ciesielski, M., Szajewski, M., Walczak, J., Pęksa, R., Lenckowski, R., Supeł, M., Zieliński, J., Kruszewski, W. J."Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma". Oncology Letters 21, no. 2 (2021): 142. https://doi.org/10.3892/ol.2020.12403