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Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3

  • Authors:
    • Yanli He
    • Haiyan Nan
    • Linfeng Yan
    • Tao Ma
    • Minghao Man
    • Bo Tian
    • Shaochun Guo
    • Xingye Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 157
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    Published online on: December 31, 2020
       https://doi.org/10.3892/ol.2020.12418
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Abstract

Glioma is one of the most common and aggressive malignant intracranial tumors worldwide. Recently, non‑coding RNAs have been found to play critical roles in the development of glioma. However, the exact mechanisms have not been fully elucidated. In the present study, reverse transcription‑quantitative PCR was used to determine the expression level of the long non‑coding RNA MIR22HG and microRNA (miR)‑9, while western blot analysis was used to detect the protein expression level of CPEB3. The potential binding sites were predicted using the StarBase v2.0 online tool and the hypothesis was verified using a luciferase reporter assay. A Cell Counting Kit‑8 assay was used to assess cell viability, while wound healing and Matrigel assays were used to determine the migration and invasion ability of glioma cancer cells. The results showed that MIR22HG expression level was decreased but miR‑9 expression level was elevated in glioma tissues and cell lines. Furthermore, MIR22HG was found to sponge miR‑9, while CPEB3 was the direct target of miR‑9 in the glioma cell line. Functionally, MIR22HG regulated the proliferation, invasion and migration of the glioma cell line by targeting miR‑9. CPEB3 may be involved in the progression of the glioma cell line. Taken together, these findings confirmed that MIR22HG suppressed glioma development by inhibiting the miR‑9/CPEB3 axis and provides a novel therapeutic strategy for glioma treatment.
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Copy and paste a formatted citation
Spandidos Publications style
He Y, Nan H, Yan L, Ma T, Man M, Tian B, Guo S and Zhang X: Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3. Oncol Lett 21: 157, 2021.
APA
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B. ... Zhang, X. (2021). Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3. Oncology Letters, 21, 157. https://doi.org/10.3892/ol.2020.12418
MLA
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B., Guo, S., Zhang, X."Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3". Oncology Letters 21.2 (2021): 157.
Chicago
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B., Guo, S., Zhang, X."Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3". Oncology Letters 21, no. 2 (2021): 157. https://doi.org/10.3892/ol.2020.12418
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Nan H, Yan L, Ma T, Man M, Tian B, Guo S and Zhang X: Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3. Oncol Lett 21: 157, 2021.
APA
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B. ... Zhang, X. (2021). Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3. Oncology Letters, 21, 157. https://doi.org/10.3892/ol.2020.12418
MLA
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B., Guo, S., Zhang, X."Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3". Oncology Letters 21.2 (2021): 157.
Chicago
He, Y., Nan, H., Yan, L., Ma, T., Man, M., Tian, B., Guo, S., Zhang, X."Long non‑coding RNA MIR22HG inhibits glioma progression by downregulating microRNA‑9/CPEB3". Oncology Letters 21, no. 2 (2021): 157. https://doi.org/10.3892/ol.2020.12418
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