Phosphorylated form of pyruvate dehydrogenase α1 mediates tumor necrosis factor α‑induced glioma cell migration
- Zijun Yang
- Yidan Wang
- Li Zhang
- Chenjin Zhao
- Donglin Wang
Affiliations: Department of Pathology, Medical College, Nantong University, Nantong, Jiangsu 226002, P.R. China, Center for Health Management, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226002, P.R. China, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu 226002, P.R. China, Department of Cerebral Surgery, The Second People's Hospital of Nantong, Nantong, Jiangsu 226002, P.R. China
- Published online on: January 6, 2021 https://doi.org/10.3892/ol.2021.12437
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Cell migration is an important factor influencing the treatment outcomes of high‑grade glioma (World Health Organization grades III‑IV). Using immunohistochemical staining, the present study demonstrated that the protein levels of phosphorylated pyruvate dehydrogenase α1 (p‑PDHA1) were increased according to the grade of glioma. Moreover, p‑PDHA1 mediated tumor necrosis factor‑α (TNF‑α)‑induced cell migration in glioma cells. Phalloidin staining and western blot analysis were used to detect the protein level of p‑PDHA1 in U251 glioma cells stimulated by TNF‑α at different time points. Phalloidin staining was used to observe the cytoskeletal structure. The effects on the expression of specific migration markers and on the cytoskeletal structure were also detected. Dichloroacetic acid is an inhibitor of PDK. These results indicated that p‑PDHA1 served an important role in the migration of glioma cells, and consequently in the development of glioma.