Sarcoma‑180 tumor affects the quality of oocytes in mice

Chen,Zihang; Wang,Simin; Luo,Xuexia; Yang,Yanhong

doi:10.3892/ol.2021.12442

Sarcoma‑180 tumor affects the quality of oocytes in mice

Authors:
- Zihang Chen
- Simin Wang
- Xuexia Luo
- Yanhong Yang
View Affiliations

Affiliations: The First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China
Published online on: January 6, 2021 https://doi.org/10.3892/ol.2021.12442
Article Number: 181
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Numerous factors can affect the quality of oocytes; however, the effects of cancer on the quality of oocytes and the underlying mechanisms remain unclear. In the present study, the effects of the sarcoma‑180 (S‑180) cell line on the quality of oocytes were investigated using S‑180 tumor‑bearing mice. In total, 42 female C57BL/6J mice were randomly divided into the tumor‑bearing group and the control group, with 21 mice per group. The weight of the mice and ovaries were recorded, and blood glucose, serum insulin, lipopolysaccharide, triglyceride (TG) and total cholesterol (TC) levels were analyzed using the corresponding detection kits. Hematoxylin and eosin staining was performed to observe the pathological changes of the ovarian tissue, and reverse transcription‑quantitative PCR (RT‑qPCR) was used to analyze the expression levels of meiosis arrest female 1 (MARF1), SUMO‑specific protease 7 (SENP7), aralkylamine N‑acetyltransferase (AANAT), cell division cycle 25B and glycine‑rich protein 3. The results of the present study revealed that the number of oocytes in the two groups of mice was similar; however, the number of abnormal oocytes was increased in the tumor‑bearing group. The serum levels of TG and TC were significantly elevated in the tumor‑bearing group compared with in the control group (P<0.01). Additionally, RT‑qPCR analysis demonstrated that the expression levels of SENP7 were downregulated, while the expression levels of MARF1 and AANAT were upregulated in the ovaries of the tumor‑bearing group compared with in the control group (P<0.01). In conclusion, the findings of the present study suggested that cancer may affect the reproductive system of mice and decrease the quality of oocytes by regulating the expression levels of reproduction‑associated genes. These results provided novel insights into the reproductive ability of patients with cancer.

View Figures

View References

1	Global Burden of Disease Cancer Collaboration, . Fitzmaurice C, Allen C, Barber RM, Barregard L, Bhutta ZA, Brenner H, Dicker DJ, Chimed-Orchir O, Dandona R, et al: Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: A systematic analysis for the global burden of disease study. JAMA Oncol. 3:524–548. 2017. View Article : Google Scholar : PubMed/NCBI
2	Bhanushali AA, Raghunathan R, Kalraiya RD and Mehta NG: Cancer-related anemia in a rat model: Alpha2-macroglobulin from Yoshida sarcoma shortens erythrocyte survival. Eur J Haematol. 68:42–48. 2015. View Article : Google Scholar
3	Huot JR, Novinger LJ, Pin F, Narasimhan A, Zimmers TA, O'Connell TM and Bonetto A: Formation of colorectal liver metastases induces musculoskeletal and metabolic abnormalities consistent with exacerbated cachexia. JCI Insight. 5:1366872020. View Article : Google Scholar : PubMed/NCBI
4	Kao HT, Buka SL, Kelsey KT, Gruber DF and Porton B: The correlation between rates of cancer and autism: An exploratory ecological investigation. PLoS One. 5:e93722010. View Article : Google Scholar : PubMed/NCBI
5	Xia Z, Su YR, Petersen P, Qi L, Kim AE, Figueiredo JC, Lin Y, Nan H, Sakoda LC, Albanes D, et al: Functional informed genome-wide interaction analysis of body mass index, diabetes and colorectal cancer risk. Cancer Med. 9:3563–3573. 2020. View Article : Google Scholar : PubMed/NCBI
6	Dunning KR and Robker RL: Promoting lipid utilization with l-carnitine to improve oocyte quality. Anim Reprod Sci. 134:69–75. 2012. View Article : Google Scholar : PubMed/NCBI
7	Alves JPM, Fernandes CCL, Rossetto R, Silva CPD, Galvão ITOM, Bertolini M and Rondina D: Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats. Reprod Domest Anim. 54:1206–1216. 2019. View Article : Google Scholar : PubMed/NCBI
8	Yu S, Zhao Y, Feng Y, Zhang H, Li L, Shen W, Zhao M and Min L: β-carotene improves oocyte development and maturation under oxidative stress in vitro. in vitro Cell Dev Biol Anim. 55:548–558. 2019. View Article : Google Scholar : PubMed/NCBI
9	Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, Aitken RJ and Nixon B: Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity. J Biol Chem. 293:18944–18964. 2018. View Article : Google Scholar : PubMed/NCBI
10	Leem J, Bai GY, Kim JS and Oh JS: Melatonin protects mouse oocytes from DNA damage by enhancing nonhomologous end-joining repair. J Pineal Res. 67:e126032019. View Article : Google Scholar : PubMed/NCBI
11	Rao A, Nayak G, Kumari S, Kalthur SG, Mutalik SP, Mutalik S, Adiga SK and Kalthur G: Exposure to first line anti-tuberculosis drugs in prepubertal age reduces the quality and functional competence of spermatozoa and oocytes in Swiss albino mice. Environ Toxicol Pharmacol. 73:1032922020. View Article : Google Scholar : PubMed/NCBI
12	Patel SG and Ahnen DJ: Colorectal cancer in the young. Curr Gastroenterol Rep. 20:152018. View Article : Google Scholar : PubMed/NCBI
13	Moore K and Brewer MA: Endometrial cancer: Is this a new disease? Am Soc Clin Oncol Educ Book. 37:435–442. 2017. View Article : Google Scholar : PubMed/NCBI
14	Raab M, Kappel S, Krämer A, Sanhaji M, Matthess Y, Kurunci-Csacsko E, Calzada-Wack J, Rathkolb B, Rozman J, Adler T, et al: Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells. Nat Commun. 2:3952011. View Article : Google Scholar : PubMed/NCBI
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
16	He YT, Yang LL, Zhao Y, Shen W, Yin S and Sun QY: Fenoxaprop-ethyl affects mouse oocyte quality and the underlying mechanisms. Pest Manag Sci. 75:844–851. 2019. View Article : Google Scholar : PubMed/NCBI
17	Brantley KD, Riis AH, Erichsen R, Thorlacius-Ussing O, Møller HJ and Lash TL: The association of serum lipid levels with colorectal cancer recurrence. Cancer Epidemiol. 66:1017252020. View Article : Google Scholar : PubMed/NCBI
18	Wang S, Wang J, Jiang Y and Jiang W: Association between blood lipid level and embryo quality during in vitro fertilization. Medicine (Baltimore). 99:e196652020. View Article : Google Scholar : PubMed/NCBI
19	Xiang JW, Xiao Y, Gan Y, Chen H, Liu Y, Wang L, Nie Q, Liu F, Gong X, Fu JL, et al: Glucose oxidase- and UVA-induced changes in the expression patterns of seven de-sumoylation enzymes (SENPs) are associated with cataract development. Curr Mol Med. 19:48–53. 2019. View Article : Google Scholar : PubMed/NCBI
20	Garvin AJ, Densham RM, Blair-Reid SA, Pratt KM, Stone HR, Weekes D, Lawrence KJ and Morris JR: The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair. EMBO Rep. 14:975–983. 2013. View Article : Google Scholar : PubMed/NCBI
21	Huang CJ, Wu D, Jiao XF, Khan FA, Xiong CL, Liu XM, Yang J, Yin TL and Huo LJ: Maternal SENP7 programs meiosis architecture and embryo survival in mouse. Biochim Biophys Acta Mol Cell Res. 1864:1195–1206. 2017. View Article : Google Scholar : PubMed/NCBI
22	Su YQ, Sugiura K, Sun F, Pendola JK, Cox GA, Handel MA, Schimenti JC and Eppig JJ: MARF1 regulates essential oogenic processes in mice. Science. 335:1496–1499. 2012. View Article : Google Scholar : PubMed/NCBI
23	Cao GY, Li MZ, Wang H, Shi LY and Su YQ: Interference with the C-terminal structure of MARF1 causes defective oocyte meiotic division and female infertility in mice. J Biomed Res. 32:58–67. 2018.PubMed/NCBI
24	Yao Q, Cao G, Li M, Wu B, Zhang X, Zhang T, Guo J, Yin H, Shi L, Chen J, et al: Ribonuclease activity of MARF1 controls oocyte RNA homeostasis and genome integrity in mice. Proc Natl Acad Sci USA. 115:11250–11255. 2018. View Article : Google Scholar : PubMed/NCBI
25	Sakaguchi K, Itoh MT, Takahashi N, Tarumi W and Ishizuka B: The rat oocyte synthesises melatonin. Reprod Fertil Dev. 25:674–682. 2013. View Article : Google Scholar : PubMed/NCBI
26	Tamura H, Jozaki M, Tanabe M, Shirafuta Y, Mihara Y, Shinagawa M, Tamura I, Maekawa R, Sato S, Taketani T, et al: Importance of melatonin in assisted reproductive technology and ovarian aging. Int J Mol Sci. 21:11352020. View Article : Google Scholar
27	Kang H, Hwang SC, Park YS and Oh JS: Cdc25B phosphatase participates in maintaining metaphase II arrest in mouse oocytes. Mol Cells. 35:514–518. 2013. View Article : Google Scholar : PubMed/NCBI
28	Firmani LD, Uliasz TF and Mehlmann LM: The switch from cAMP-independent to cAMP-dependent arrest of meiotic prophase is associated with coordinated GPR3 and CDK1 expression in mouse oocytes. Dev Biol. 434:196–205. 2018. View Article : Google Scholar : PubMed/NCBI