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MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α

  • Authors:
    • Sweta Raikundalia
    • Siti Aisyah Faten Mohamed Sa'dom
    • Ling Ling Few
    • Wei Cun See Too
  • View Affiliations / Copyright

    Affiliations: School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia
    Copyright: © Raikundalia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 183
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    Published online on: January 6, 2021
       https://doi.org/10.3892/ol.2021.12444
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Abstract

Choline kinase (ChK) catalyzes the first step in the CDP‑choline pathway for the synthesis of phosphatidylcholine. The α isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attention being paid to ChK expression, as well as its activity and inhibition in cancer, there are only limited studies available on the regulation of ChK, including its regulation by microRNAs (miRNAs/miRs). The dysregulation of gene expression by miRNAs is a common cause for carcinogenesis. In the present study, miR‑367‑3p was predicted to target the 3'‑untranslated region (UTR) of the ChK α (chka) mRNA transcript. The binding of miR‑367‑3p to the 3'‑UTR of chka was validated by a luciferase assay. The effects of the miR‑367‑3p mimic on chka gene and protein expression levels were determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. miR‑367‑3p significantly downregulated the expression of chka to ~60% of the negative control. Cells transfected with miR‑367‑3p exhibited higher levels of apoptosis and a lower cell migration compared with the control. To the best of our knowledge, the present study provided the first experimental evidence of the regulation of chka expression by miR‑367‑3p. The pro‑apoptotic and suppressive effects of miR‑367‑3p on cell migration were similar to the anticancer effects resulting from the inhibition of ChK enzyme activity or the knockdown of chka gene expression by small interfering RNA. Therefore, these findings may potentially lead to the use of miR‑367‑3p in anticancer strategies that target ChK.
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Copy and paste a formatted citation
Spandidos Publications style
Raikundalia S, Mohamed Sa'dom S, Few LL and See Too W: MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α. Oncol Lett 21: 183, 2021.
APA
Raikundalia, S., Mohamed Sa'dom, S., Few, L.L., & See Too, W. (2021). MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α. Oncology Letters, 21, 183. https://doi.org/10.3892/ol.2021.12444
MLA
Raikundalia, S., Mohamed Sa'dom, S., Few, L. L., See Too, W."MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α". Oncology Letters 21.3 (2021): 183.
Chicago
Raikundalia, S., Mohamed Sa'dom, S., Few, L. L., See Too, W."MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α". Oncology Letters 21, no. 3 (2021): 183. https://doi.org/10.3892/ol.2021.12444
Copy and paste a formatted citation
x
Spandidos Publications style
Raikundalia S, Mohamed Sa'dom S, Few LL and See Too W: MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α. Oncol Lett 21: 183, 2021.
APA
Raikundalia, S., Mohamed Sa'dom, S., Few, L.L., & See Too, W. (2021). MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α. Oncology Letters, 21, 183. https://doi.org/10.3892/ol.2021.12444
MLA
Raikundalia, S., Mohamed Sa'dom, S., Few, L. L., See Too, W."MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α". Oncology Letters 21.3 (2021): 183.
Chicago
Raikundalia, S., Mohamed Sa'dom, S., Few, L. L., See Too, W."MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α". Oncology Letters 21, no. 3 (2021): 183. https://doi.org/10.3892/ol.2021.12444
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