Open Access

Bioinformatics and immunohistochemistry analyses of expression levels and clinical significance of CXCL2 and TANs in an oral squamous cell carcinoma tumor microenvironment of Prophyromonas gingivalis infection

  • Authors:
    • Zhi-Chen Guo
    • Sakendeke Jumatai
    • Si-Li Jing
    • Lu-Lu Hu
    • Xin-Yu Jia
    • Zhong-Cheng Gong
  • View Affiliations

  • Published online on: January 6, 2021     https://doi.org/10.3892/ol.2021.12450
  • Article Number: 189
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to detect the immunoexpression and clinical significance of Porphyromonas gingivalis (P. gingivalis) in the tumor microenvironment (TME) of oral squamous cell carcinoma (OSCC). The immunoexpression of P. gingivalis in OSCC tissues was detected via immunohistochemistry (IHC) after P. gingivalis was infected into the TME of OSCC. To identify the differentially expressed genes in the carcinogenesis and progression of OSCC with P. gingivalis infection, microarray datasets (GSE87539 and GSE138206) were downloaded from the Gene Expression Omnibus database. The immunoexpression levels of C‑X‑C motif chemokine ligand 2 (CXCL2) and tumor‑associated neutrophils (TANs) were also evaluated via IHC, and the immunoexpression levels of all three clinical variables were analyzed using χ2 or Fisher's exact tests. The survival rates were calculated using the Kaplan‑Meier method and the survival curves were compared using log‑rank tests. Predominantly strong immunoexpression of P. gingivalis was identified in OSCC samples. CXCL2 was considered to be a differential gene in the two datasets. Immunoexpression of P. gingivalis was positively associated with CXCL2 and TANs expression. Furthermore, P. gingivalis was associated with survival status (P<0.001) and differentiation (P<0.001). CXCL2 was associated with age (P=0.038) and survival status (P=0.003), while TANs were associated with T stage (P=0.015) and clinical stage (P=0.002). These clinical variables were considered to be independent risk factors for the poor prognosis of patients with OSCC. Collectively, the results suggested that the immunoexpression of P. gingivalis may be positively associated with CXCL2 and TANs. In addition, the strong immunoexpression levels of P. gingivalis, CXCL2 and TANs may be associated with a poor prognosis in patients with OSCC.
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March-2021
Volume 21 Issue 3

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Spandidos Publications style
Guo Z, Jumatai S, Jing S, Hu L, Jia X and Gong Z: Bioinformatics and immunohistochemistry analyses of expression levels and clinical significance of CXCL2 and TANs in an oral squamous cell carcinoma tumor microenvironment of <em>Prophyromonas gingivalis</em> infection. Oncol Lett 21: 189, 2021
APA
Guo, Z., Jumatai, S., Jing, S., Hu, L., Jia, X., & Gong, Z. (2021). Bioinformatics and immunohistochemistry analyses of expression levels and clinical significance of CXCL2 and TANs in an oral squamous cell carcinoma tumor microenvironment of <em>Prophyromonas gingivalis</em> infection. Oncology Letters, 21, 189. https://doi.org/10.3892/ol.2021.12450
MLA
Guo, Z., Jumatai, S., Jing, S., Hu, L., Jia, X., Gong, Z."Bioinformatics and immunohistochemistry analyses of expression levels and clinical significance of CXCL2 and TANs in an oral squamous cell carcinoma tumor microenvironment of <em>Prophyromonas gingivalis</em> infection". Oncology Letters 21.3 (2021): 189.
Chicago
Guo, Z., Jumatai, S., Jing, S., Hu, L., Jia, X., Gong, Z."Bioinformatics and immunohistochemistry analyses of expression levels and clinical significance of CXCL2 and TANs in an oral squamous cell carcinoma tumor microenvironment of <em>Prophyromonas gingivalis</em> infection". Oncology Letters 21, no. 3 (2021): 189. https://doi.org/10.3892/ol.2021.12450