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Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL

  • Authors:
    • Xunlei Pang
    • Yanhong Wang
    • Bei Miao
    • Sujuan Fei
    • Weichang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215008, P.R. China, Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
    Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 196
    |
    Published online on: January 11, 2021
       https://doi.org/10.3892/ol.2021.12457
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Abstract

Colorectal cancer (CRC) is the third most common malignant disease in adults. ADP ribosylation factor‑like GTPase 2 (ARL2) is crucial for controlling the dynamics of microtubules and mitochondrial functions. However, the biological function of ARL2 in CRC remains unclear. The present study was performed to identify the expression level and functional role of ARL2 in CRC. A total of 19 CRC and 3 normal healthy colorectal tissues were collected. Furthermore, ARL2 expression was analyzed in healthy colorectal and CRC tissues by immunohistochemistry (IHC). ARL2 overexpression and knockdown was achieved using lentiviral vectors and plasmid transfection in HCT8 and HCT116 cells. The protein and mRNA expression levels of ARL2 and AXL were analyzed using western blot and reverse transcription‑quantitative PCR in ARL2 knockdown and ARL2 overexpressing HCT8 and HCT116 cells. Cell Counting Kit‑8, colony formation, wound healing, and Matrigel assays were used to investigate the biological functions of ARL2. Taken together, ARL2 protein expression level was upregulated in CRC tissues. Furthermore, ARL2 overexpression decreased proliferation and weakened the colony‑formation abilities of the CRC cells, as well as their migratory and invasive abilities. ARL2 interference enhanced proliferation and colony‑formation rates of the CRC cells, as well as their migratory and invasive abilities. ARL2 regulated CRC proliferation and tumorigenicity and was negatively associated with AXL. The results of the present study suggested that the proliferation, migration and tumorigenicity of the CRC cells could be inhibited by ARL2 overexpression. The latter may be used as a predicted and potential therapeutic target for CRC.
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View References

1 

Mattiuzzi C, Sanchis-Gomar F and Lippi G: Concise update on colorectal cancer epidemiology. Ann Transl Med. 7:609. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Gu X, Zheng R, Xia C, Zeng H, Zhang S, Zou X, Yang Z, Li H and Chen W: Interactions between life expectancy and the incidence and mortality rates of cancer in China: A population-based cluster analysis. Cancer Commun (Lond). 38:442018. View Article : Google Scholar : PubMed/NCBI

3 

Zeng H, Chen W, Zheng R, Zhang S, Ji JS, Zou X, Xia C, Sun K, Yang Z, Li H, et al: Changing cancer survival in China during 2003-15: A pooled analysis of 17 population-based cancer registries. Lancet Glob Health. 6:e555–e567. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Dai W, Li Y, Mo S, Feng Y, Zhang L, Xu Y, Li Q and Cai G: A robust gene signature for the prediction of early relapse in stage I–III colon cancer. Mol Oncol. 12:463–475. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Chen K, Koe CT, Xing ZB, Tian X, Rossi F, Wang C, Tang Q, Zong W, Hong WJ, Taneja R, et al: Arl2- and Msps-dependent microtubule growth governs asymmetric division. J Cell Biol. 212:661–676. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Ozdemir ES, Jang H, Gursoy A, Keskin O and Nussinov R: Arl2-mediated allosteric release of farnesylated KRas4B from shuttling factor PDEδ. J Phys Chem B. 122:7503–7513. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Stettin D, Waldmann A, Wolters M, Trunz B, Schauder P and Hahn A: Infection with Helicobacter pylori-outcome of a cross-sectional investigation. Dtsch Med Wochenschr. 132:2677–2682. 2007.(In German). View Article : Google Scholar : PubMed/NCBI

8 

Zhang F and Cheong JK: The renewed battle against RAS-mutant cancers. Cell Mol Life Sci. 73:1845–1858. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Cox AD, Fesik SW, Kimmelman AC, Luo J and Der CJ: Drugging the undruggable RAS: Mission possible? Nat Rev Drug Discov. 13:828–851. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Stephen AG, Esposito D, Bagni RK and McCormick F: Dragging ras back in the ring. Cancer Cell. 25:272–281. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Wang Y, Guan G, Cheng W, Jiang Y, Shan F, Wu A, Cheng P and Guo Z: ARL2 overexpression inhibits glioma proliferation and tumorigenicity via down-regulating AXL. BMC Cancer. 18:5992018. View Article : Google Scholar : PubMed/NCBI

12 

Li HJ, Sun XM, Li ZK, Yin QW, Pang H, Pan JJ, Li X and Chen W: LncRNA UCA1 promotes mitochondrial function of bladder cancer via the MiR-195/ARL2 signaling pathway. Cell Physiol Biochem. 43:2548–2561. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Béghin A, Matera E, Brunet-Manquat S and Dumontet C: Expression of Arl2 is associated with p53 localization and chemosensitivity in a breast cancer cell line. Cell Cycle. 7:3074–3082. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Hass HG, Vogel U, Scheurlen M and Jobst J: Gene-expression analysis identifies specific patterns of dysregulated molecular pathways and genetic subgroups of human hepatocellular carcinoma. Anticancer Res. 36:5087–5096. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Peng R, Men J, Ma R, Wang Q, Wang Y, Sun Y and Ren J: miR-214 down-regulates ARL2 and suppresses growth and invasion of cervical cancer cells. Biochem Biophys Res Commun. 484:623–630. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Martinelli E, Martini G, Cardone C, Troiani T, Liguori G, Vitagliano D, Napolitano S, Morgillo F, Rinaldi B, Melillo RM, et al: AXL is an oncotarget in human colorectal cancer. Oncotarget. 6:23281–23296. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Uribe DJ, Mandell EK, Watson A, Martinez JD, Leighton JA, Ghosh S and Rothlin CV: The receptor tyrosine kinase AXL promotes migration and invasion in colorectal cancer. PLoS One. 12:e01799792017. View Article : Google Scholar : PubMed/NCBI

18 

Bläker H: Grading of tumors in the tubular digestive tract: Esophagus, stomach, colon and rectum. Pathologe. 37:293–298. 2016.(In German). View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Hou P, Li L, Chen F, Chen Y, Liu H, Li J, Bai J and Zheng J: PTBP3-mediated regulation of ZEB1 mRNA stability promotes epithelial-mesenchymal transition in breast cancer. Cancer Res. 78:387–398. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Qiu C, Bu X and Jiang Z: Protocadherin-10 acts as a tumor suppressor gene, and is frequently downregulated by promoter methylation in pancreatic cancer cells. Oncol Rep. 36:383–389. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Tohidi F, Sadat SM, Bolhassani A and Yaghobi R: Construction and production of HIV–VLP harboring MPER-V3 for potential vaccine study. Curr HIV Res. 15:434–439. 2017.PubMed/NCBI

23 

Seetharaman S and Etienne-Manneville S: Microtubules at focal adhesions-a double-edged sword. J Cell Sci. 132:jcs2328432019. View Article : Google Scholar : PubMed/NCBI

24 

van Vuuren RJ, Botes M, Jurgens T, Joubert AM and van den Bout I: Novel sulphamoylated 2-methoxy estradiol derivatives inhibit breast cancer migration by disrupting microtubule turnover and organization. Cancer Cell Int. 19:12019. View Article : Google Scholar : PubMed/NCBI

25 

Galmarini CM, Martin M, Bouchet BP, Guillen-Navarro MJ, Martínez-Diez M, Martinez-Leal JF, Akhmanova A and Aviles P: Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells. BMC Cancer. 18:1642018. View Article : Google Scholar : PubMed/NCBI

26 

Long LM, He BF, Huang GQ, Guo YH, Liu YS and Huo JR: microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2. Oncol Lett. 9:645–650. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Zhou C, Cunningham L, Marcus AI, Li Y and Kahn RA: Arl2 and Arl3 regulate different microtubule-dependent processes. Mol Biol Cell. 17:2476–2487. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Newman LE, Schiavon CR, Zhou C and Kahn RA: The abundance of the ARL2 GTPase and its GAP, ELMOD2, at mitochondria are modulated by the fusogenic activity of mitofusins and stressors. PLoS One. 12:e01751642017. View Article : Google Scholar : PubMed/NCBI

29 

Song X, Akasaka H and Wang H, Abbasgholizadeh R, Shin JH, Zang F, Chen J, Logsdon CD, Maitra A, Bean AJ and Wang H: Hematopoietic progenitor kinase 1 down-regulates the oncogenic receptor tyrosine kinase AXL in pancreatic cancer. J Biol Chem. 295:2348–2358. 2020. View Article : Google Scholar : PubMed/NCBI

30 

Bae CA, Ham IH, Oh HJ, Lee D, Woo J, Son SY, Yoon JH, Lorens JB, Brekken RA, Kim TM, et al: Inhibiting the GAS6/AXL axis suppresses tumor progression by blocking the interaction between cancer-associated fibroblasts and cancer cells in gastric carcinoma. Gastric Cancer. 23:824–836. 2020. View Article : Google Scholar : PubMed/NCBI

31 

Vajkoczy P, Knyazev P, Kunkel A, Capelle HH, Behrndt S, von Tengg-Kobligk H, Kiessling F, Eichelsbacher U, Essig M, Read TA, et al: Dominant-negative inhibition of the Axl receptor tyrosine kinase suppresses brain tumor cell growth and invasion and prolongs survival. Proc Natl Acad Sci USA. 103:5799–5804. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Cheng P, Phillips E, Kim SH, Taylor D, Hielscher T, Puccio L, Hjelmeland AB, Lichter P, Nakano I and Goidts V: Kinome-wide shRNA screen identifies the receptor tyrosine kinase AXL as a key regulator for mesenchymal glioblastoma stem-like cells. Stem Cell Reports. 4:899–913. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pang X, Wang Y, Miao B, Fei S and Chen W: Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL. Oncol Lett 21: 196, 2021.
APA
Pang, X., Wang, Y., Miao, B., Fei, S., & Chen, W. (2021). Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL. Oncology Letters, 21, 196. https://doi.org/10.3892/ol.2021.12457
MLA
Pang, X., Wang, Y., Miao, B., Fei, S., Chen, W."Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL". Oncology Letters 21.3 (2021): 196.
Chicago
Pang, X., Wang, Y., Miao, B., Fei, S., Chen, W."Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL". Oncology Letters 21, no. 3 (2021): 196. https://doi.org/10.3892/ol.2021.12457
Copy and paste a formatted citation
x
Spandidos Publications style
Pang X, Wang Y, Miao B, Fei S and Chen W: Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL. Oncol Lett 21: 196, 2021.
APA
Pang, X., Wang, Y., Miao, B., Fei, S., & Chen, W. (2021). Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL. Oncology Letters, 21, 196. https://doi.org/10.3892/ol.2021.12457
MLA
Pang, X., Wang, Y., Miao, B., Fei, S., Chen, W."Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL". Oncology Letters 21.3 (2021): 196.
Chicago
Pang, X., Wang, Y., Miao, B., Fei, S., Chen, W."Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL". Oncology Letters 21, no. 3 (2021): 196. https://doi.org/10.3892/ol.2021.12457
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