Open Access

Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway

  • Authors:
    • Wenqi Huang
    • Hang Sun
    • Ting Hu
    • Dongju Zhu
    • Xianli Long
    • Hui Guo
    • Qi Liu
  • View Affiliations

  • Published online on: January 12, 2021     https://doi.org/10.3892/ol.2021.12458
  • Article Number: 197
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multiple myeloma (MM) is the second most common haematological malignancy and remains an incurable disease, with most patients relapsing and requiring further treatment. Augmenter of liver regeneration (ALR) is a vital protein affecting fundamental processes such as energy transduction, cell survival and regeneration. Silencing ALR inhibits cell proliferation and triggers apoptosis in human MM U266 cells. However, little is known about the role of 15‑kDa‑ALR on MM. In the present study, the role of 15‑kDa‑ALR in human MM cells was investigated. Blocking extracellular 15‑kDa‑ALR with an anti‑ALR monoclonal antibody (McAb) decreased the proliferation and viability of U266 cells. However, the results of flow cytometry revealed no changes in apoptosis, and the expression levels of Bax, Bcl‑2, caspase‑3 and cleaved caspase‑3 were not affected. However, combined treatment with anti‑ALR McAb and epirubicin increased the apoptosis of U266 cells. RNA sequencing results indicated that the ERK1/2, JNK‑MAPK and STAT3 signaling pathways, as well as the cell cycle, were associated with the mechanism of action of the anti‑ALR McAb, and PCR, western blotting and cell cycle analysis confirmed these results. The present findings suggested that blocking extracellular 15‑kDa‑ALR in U266 cells with an anti‑ALR McAb decreased cell proliferation via the MAPK, STAT3 and cell cycle signaling pathways without increasing apoptosis. Thus, 15‑kDa‑ALR may be a new therapeutic target for myeloma.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-1074
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Spandidos Publications style
Huang W, Sun H, Hu T, Zhu D, Long X, Guo H and Liu Q: Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway. Oncol Lett 21: 197, 2021
APA
Huang, W., Sun, H., Hu, T., Zhu, D., Long, X., Guo, H., & Liu, Q. (2021). Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway. Oncology Letters, 21, 197. https://doi.org/10.3892/ol.2021.12458
MLA
Huang, W., Sun, H., Hu, T., Zhu, D., Long, X., Guo, H., Liu, Q."Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway". Oncology Letters 21.3 (2021): 197.
Chicago
Huang, W., Sun, H., Hu, T., Zhu, D., Long, X., Guo, H., Liu, Q."Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway". Oncology Letters 21, no. 3 (2021): 197. https://doi.org/10.3892/ol.2021.12458