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Article Open Access

Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker

  • Authors:
    • Xiao-Cong Mo
    • Zi-Tong Zhang
    • Meng-Jia Song
    • Zi-Qi Zhou
    • Jian-Xiong Zeng
    • Yu-Fei Du
    • Feng-Ze Sun
    • Jie-Ying Yang
    • Jun-Yi He
    • Yue Huang
    • Jian-Chuan Xia
    • De-Sheng Weng
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Oncology in South China, Collaborative Innovation Centre for Cancer Medicine, Guangzhou, Guangdong 510060, P.R. China
    Copyright: © Mo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 205
    |
    Published online on: January 14, 2021
       https://doi.org/10.3892/ol.2021.12466
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Abstract

Bladder cancer (BC) is the ninth most common lethal malignancy worldwide. Great efforts have been devoted to clarify the pathogenesis of BC, but the underlying molecular mechanisms remain unclear. To screen for the genes associated with the progression and carcinogenesis of BC, three datasets were obtained from the Gene Expression Omnibus. A total of 37 tumor and 16 non‑cancerous samples were analyzed to identify differentially expressed genes (DEGs). Subsequently, 141 genes were identified, including 55 upregulated and 86 downregulated genes. The protein‑protein interaction network was established using the Search Tool for Retrieval of Interacting Genes database. Hub gene identification and module analysis were performed using Cytoscape software. Hierarchical clustering of hub genes was conducted using the University of California, Santa Cruz Cancer Genomics Browser. Among the hub genes, kinesin family member 11 (KIF11) was identified as one of the most significant prognostic biomarkers among all the candidates. The Kaplan Meier Plotter database was used for survival analysis of KIF11. The expression profile of KIF11 was analyzed using the ONCOMINE database. The expression levels of KIF11 in BC samples and bladder cells were measured using reverse transcription‑quantitative pCR, immunohistochemistry and western blotting. In summary, KIF11 was significantly upregulated in BC and might act as a potential prognostic biomarker. The present identification of DEGs and hub genes in BC may provide novel insight for investigating the molecular mechanisms of BC.
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Copy and paste a formatted citation
Spandidos Publications style
Mo X, Zhang Z, Song M, Zhou Z, Zeng J, Du Y, Sun F, Yang J, He J, Huang Y, Huang Y, et al: Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker. Oncol Lett 21: 205, 2021.
APA
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y. ... Weng, D. (2021). Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker. Oncology Letters, 21, 205. https://doi.org/10.3892/ol.2021.12466
MLA
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y., Sun, F., Yang, J., He, J., Huang, Y., Xia, J., Weng, D."Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker". Oncology Letters 21.3 (2021): 205.
Chicago
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y., Sun, F., Yang, J., He, J., Huang, Y., Xia, J., Weng, D."Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker". Oncology Letters 21, no. 3 (2021): 205. https://doi.org/10.3892/ol.2021.12466
Copy and paste a formatted citation
x
Spandidos Publications style
Mo X, Zhang Z, Song M, Zhou Z, Zeng J, Du Y, Sun F, Yang J, He J, Huang Y, Huang Y, et al: Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker. Oncol Lett 21: 205, 2021.
APA
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y. ... Weng, D. (2021). Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker. Oncology Letters, 21, 205. https://doi.org/10.3892/ol.2021.12466
MLA
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y., Sun, F., Yang, J., He, J., Huang, Y., Xia, J., Weng, D."Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker". Oncology Letters 21.3 (2021): 205.
Chicago
Mo, X., Zhang, Z., Song, M., Zhou, Z., Zeng, J., Du, Y., Sun, F., Yang, J., He, J., Huang, Y., Xia, J., Weng, D."Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker". Oncology Letters 21, no. 3 (2021): 205. https://doi.org/10.3892/ol.2021.12466
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