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Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma

  • Authors:
    • Zhide Liu
    • Cheng Yan
    • Yangyan Xiao
    • Weichang Zhang
    • Li Wang
    • Qinglong Li
    • Wenwu Cai
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Oncology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 234
    |
    Published online on: January 26, 2021
       https://doi.org/10.3892/ol.2021.12495
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Abstract

The p53‑upregulated modulator of apoptosis (PUMA) has been reported to be involved in various types of cancer. However, its potential biological role in gallbladder carcinoma (GBC) has not been fully elucidated. The present study aimed to determine the expression levels of PUMA and its biological effects on GBC. The mRNA and protein expression levels of PUMA in GBC tissues and cell lines were measured using reverse transcription‑quantitative PCR and western blotting, respectively. The effects of PUMA overexpression on cell viability, proliferation and invasive ability were determined in vitro using the MTT, colony formation and Transwell invasion assays, respectively. The apoptotic rates were detected using the Annexin V‑FITC apoptosis detection kit. Furthermore, follow‑up of patients with GBC was performed to identify the association between PUMA expression levels and GBC prognosis. The results of the present study demonstrated that the expression levels of PUMA were significantly lower in the GBC tissues and cell lines compared with those in adjacent normal gallbladder tissues and normal gallbladder cells, respectively. Further experiments indicated that overexpression of PUMA inhibited the viability, proliferation and invasive ability of GBC cells compared with those in the control‑transfected GBC cells. In addition, overexpression of PUMA significantly promoted apoptosis in GBC cells. Furthermore, overexpression of PUMA inhibited epithelial‑mesenchymal transition, and promoted Bax upregulation and Bcl‑2 downregulation compared with those in the control group. Low PUMA expression levels were associated with a short overall survival time in patients with GBC. In conclusions, PUMA may act as a tumor suppressor in GBC and may serve as a potential novel treatment target for human GBC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Yan C, Xiao Y, Zhang W, Wang L, Li Q and Cai W: Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma. Oncol Lett 21: 234, 2021.
APA
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., & Cai, W. (2021). Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma. Oncology Letters, 21, 234. https://doi.org/10.3892/ol.2021.12495
MLA
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., Cai, W."Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma". Oncology Letters 21.3 (2021): 234.
Chicago
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., Cai, W."Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma". Oncology Letters 21, no. 3 (2021): 234. https://doi.org/10.3892/ol.2021.12495
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Yan C, Xiao Y, Zhang W, Wang L, Li Q and Cai W: Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma. Oncol Lett 21: 234, 2021.
APA
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., & Cai, W. (2021). Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma. Oncology Letters, 21, 234. https://doi.org/10.3892/ol.2021.12495
MLA
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., Cai, W."Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma". Oncology Letters 21.3 (2021): 234.
Chicago
Liu, Z., Yan, C., Xiao, Y., Zhang, W., Wang, L., Li, Q., Cai, W."Expression and inhibitory effects of p53‑upregulated modulator of apoptosis in gallbladder carcinoma". Oncology Letters 21, no. 3 (2021): 234. https://doi.org/10.3892/ol.2021.12495
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