MicroRNA‑325‑3p contributes to colorectal carcinoma by targeting cytokeratin 18

Song,Chuanfang; Wang,Xiujie; Zhao,Xinxin; Ai,Jiang; Qi,Yixuan; Chen,Aidong

doi:10.3892/ol.2021.12509

MicroRNA‑325‑3p contributes to colorectal carcinoma by targeting cytokeratin 18

Authors:
- Chuanfang Song
- Xiujie Wang
- Xinxin Zhao
- Jiang Ai
- Yixuan Qi
- Aidong Chen
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Affiliations: Department of Gastroenterology, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China
Published online on: February 3, 2021 https://doi.org/10.3892/ol.2021.12509
Article Number: 248
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal carcinoma (CRC) is one of the most common malignant tumors. The present study aimed to investigate a non‑invasive molecular marker that can evaluate the diagnosis and potential molecular mechanism of CRC. Microarray assays and reverse transcription‑quantitative PCR analysis demonstrated that microRNA (miR)‑325‑3p expression was significantly increased in both tissues and serum samples of patients with CRC. In addition, miR‑325‑3p expression in the tissues and serum was significantly associated with differentiation, TNM stage and lymph node metastasis. The results of the dual‑luciferase reporter assay and western blot analysis revealed that cytokeratin 18 (CK18) is a target gene of miR‑325‑3p. Furthermore, treatment with transforming growth factor (TGF)‑β increased miR‑325‑3p expression in a time‑dependent manner. Conversely, TGF‑β decreased CK18 expression at 48 and 72 h. Western blot analysis demonstrated that TGF‑β1 significantly decreased the expression of the epithelial marker, CK18, and increased the expression of the mesenchymal markers, α‑SMA and vimentin. Notably, these effects were reversed following inhibition of miR‑325‑3p expression. Taken together, the results of the present study suggest that miR‑325‑3p is a key regulator of TGF‑β‑induced CK18 downregulation. Thus, elevated levels of miR‑325‑3p is an important factor affecting epithelial‑to‑mesenchymal transition, and is likely to be a molecular marker in the progression of CRC and act as a potential therapeutic target.

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