Open Access

Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence

  • Authors:
    • Jing Guo
    • Qiuyue Fang
    • Yulou Liu
    • Weiyan Xie
    • Yazhuo Zhang
    • Chuzhong Li
  • View Affiliations

  • Published online on: February 8, 2021     https://doi.org/10.3892/ol.2021.12525
  • Article Number: 264
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑functioning pituitary adenoma (NFPA) is a very common type of intracranial tumor. Monitoring and predicting the postoperative recurrence of NFPAs is difficult, as these adenomas do not present with serum hormone hypersecretion. Long non‑coding RNAs (lncRNAs) and protein‑coding genes (PCGs) play critical roles in the development and progression of numerous tumors. However, the complex network of RNA interactions related to the mechanisms underlying the postoperative recurrence of NFPA is still unclear. In the present study, 73 patients with NFPA were investigated using high‑throughput sequencing and follow‑up investigations. In total, 6 of these patients with recurrence within 1 year after surgery were selected as the fast recurrence group, and 6 patients with recurrence 5 years after surgery were selected as the slow recurrence group. By performing differential expression analysis of the fast recurrence and slow recurrence groups, a set of differentially expressed PCGs and lncRNAs were obtained (t‑test, P<0.05). Next, protein‑protein interaction coregulatory networks and lncRNA‑mRNA coexpression networks were identified. In addition, the hub lncRNA‑mRNA modules related to NFPA recurrence were further screened and transcriptome expression markers for NFPA regression were identified (log‑rank test, P<0.05). Finally, the ability of the hub and module genes to predict recurrence and progression‑free survival in patients with NFPA was evaluated. To confirm the credibility of the bioinformatic analyses, nucleolar protein 6 and LL21NC02‑21A1.1 were randomly selected from among the genes with prognostic significance for validation by reverse transcription‑quantitative PCR in another set of NFPA samples (n=9). These results may be helpful for evaluating the slow and rapid recurrence of NFPA after surgery and exploring the mechanisms underlying NFPA recurrence. Future effective biomarkers and therapeutic targets may also be revealed.
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April-2021
Volume 21 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Guo J, Fang Q, Liu Y, Xie W, Zhang Y and Li C: Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence. Oncol Lett 21: 264, 2021
APA
Guo, J., Fang, Q., Liu, Y., Xie, W., Zhang, Y., & Li, C. (2021). Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence. Oncology Letters, 21, 264. https://doi.org/10.3892/ol.2021.12525
MLA
Guo, J., Fang, Q., Liu, Y., Xie, W., Zhang, Y., Li, C."Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence". Oncology Letters 21.4 (2021): 264.
Chicago
Guo, J., Fang, Q., Liu, Y., Xie, W., Zhang, Y., Li, C."Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence". Oncology Letters 21, no. 4 (2021): 264. https://doi.org/10.3892/ol.2021.12525