AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer

Tanio,Akimitsu; Saito,Hiroaki; Amisaki,Masataka; Hara,Kazushi; Sugezawa,Ken; Uejima,Chihiro; Tada,Yoichiro; Kihara,Kyoichi; Yamamoto,Manabu; Nosaka,Kanae; Sasaki,Ryo; Osaki,Mitsuhiko; Okada,Futoshi; Fujiwara,Yoshiyuki

doi:10.3892/ol.2021.12539

April-2021 Volume 21 Issue 4

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April-2021 Volume 21 Issue 4

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Article Open Access

AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer

Authors:
- Akimitsu Tanio
- Hiroaki Saito
- Masataka Amisaki
- Kazushi Hara
- Ken Sugezawa
- Chihiro Uejima
- Yoichiro Tada
- Kyoichi Kihara
- Manabu Yamamoto
- Kanae Nosaka
- Ryo Sasaki
- Mitsuhiko Osaki
- Futoshi Okada
- Yoshiyuki Fujiwara
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Affiliations: Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Faculty of Medicine, Tottori University, Yonago, Tottori 683‑8504, Japan, Division of Organ Pathology, Faculty of Medicine, Tottori University, Yonago, Tottori 683‑8503, Japan, Division of Experimental Pathology, Faculty of Medicine, Tottori University, Yonago, Tottori 683‑8503, Japan

Copyright: © Tanio et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 278
|
Published online on: February 10, 2021

https://doi.org/10.3892/ol.2021.12539
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Abstract

Our previous study showed that adhesion molecule with immunoglobulin like domain 2 (AMIGO2) is a pivotal driver gene of liver metastasis via regulating tumor cell adhesion to liver endothelial cells in mouse models. The aim of the present study was to clarify the role of AMIGO2 in liver metastasis in patients the colorectal cancer (CRC). Two human CRC cell lines, Caco‑2 (AMIGO2‑low) and HCT116 (AMIGO2‑high), were used in this study. AMIGO2‑overexpressing Caco‑2 and AMIGO2‑knockdown HCT116 cells were generated by transfection with an AMIGO2 expression vector or AMIGO2 small interfering RNA, respectively. Cell proliferation, invasion and adhesion to human liver endothelial cells were examined in in vitro studies. Immunohistochemical analysis was also performed to evaluate the association between AMIGO2 expression and liver metastasis in patients with CRC. In vitro studies revealed that cell proliferation, invasion and adhesion to liver endothelial cells were accelerated by upregulation of AMIGO2 expression, but suppressed by downregulation of AMIGO2 expression in human CRC cells. Immunohistochemical analysis using clinical CRC specimens revealed that AMIGO2 expression was associated with the frequency of liver metastasis (P<0.01), but not that of pulmonary metastasis (P=0.611) and peritoneal dissemination (P=0.909). In addition, AMIGO2 expression levels in tumor cells were significantly higher in liver metastatic foci than primary lesions (P=0.012). In conclusion, the present results indicated that AMIGO2 expression may contribute to the formation of liver metastasis in CRC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer

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