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MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway

  • Authors:
    • Honggang Wang
    • Qingju Yang
    • Jieping Li
    • Wenping Chen
    • Xiao Jin
    • Yaowen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Gynaecology, Linyi People's Hospital, Dezhou, Shandong 251500, P.R. China, Department of Anesthesiology, Qingdao Hospital of Traditional Chinese Medicine, Qingdao University, Qingdao, Shandong 266033, P.R. China, Department of Cardiothoracic Surgery, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Rehabilitation Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 310
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    Published online on: February 22, 2021
       https://doi.org/10.3892/ol.2021.12570
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Abstract

Endometrial carcinoma (EC) is one of the most common malignant gynecological tumors. Dysregulation of microRNAs (miRNAs/miRs) is frequently identified in human tumors, playing key regulatory roles in tumor growth and metastasis. The present study aimed to explore the functions and potential mechanisms of miR‑15a‑5p in EC progression. RT‑qPCR was used to detect the expression levels of miR‑15a‑5p and vascular endothelial growth factor A (VEGFA) mRNA. Western blot analysis was performed to examine the expression of related proteins. Functional assays, including proliferation and Transwell assays were performed to determine the roles of miR‑15a‑5p in EC progression. TargetScan and luciferase reporter assays were used to explore the potential target genes of miR‑15a‑5p. The results revealed that miR‑15a‑5p was underexpressed in EC tissue samples in comparison with that in matched normal tissue samples. The expression level of miR‑15a‑5p was associated with the clinicopathologic characteristics of EC patients. Notably, both in vitro and in vivo assays revealed that miR‑15a‑5p upregulation significantly inhibited EC growth and metastasis. Furthermore, bioinformatics analysis and dual luciferase reporter assay indicated that VEGFA was a candidate target of miR‑15a‑5p. Mechanistic investigation revealed that miR‑15a‑5p inhibited EC development via regulation of Wnt/β‑catenin pathway and targeting of VEGFA. In summary, the present results demonstrated that miR‑15a‑5p could inhibit EC development and may serve as a promising therapeutic biomarker in EC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Yang Q, Li J, Chen W, Jin X and Wang Y: MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway. Oncol Lett 21: 310, 2021.
APA
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., & Wang, Y. (2021). MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway. Oncology Letters, 21, 310. https://doi.org/10.3892/ol.2021.12570
MLA
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., Wang, Y."MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway". Oncology Letters 21.4 (2021): 310.
Chicago
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., Wang, Y."MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway". Oncology Letters 21, no. 4 (2021): 310. https://doi.org/10.3892/ol.2021.12570
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Yang Q, Li J, Chen W, Jin X and Wang Y: MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway. Oncol Lett 21: 310, 2021.
APA
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., & Wang, Y. (2021). MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway. Oncology Letters, 21, 310. https://doi.org/10.3892/ol.2021.12570
MLA
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., Wang, Y."MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway". Oncology Letters 21.4 (2021): 310.
Chicago
Wang, H., Yang, Q., Li, J., Chen, W., Jin, X., Wang, Y."MicroRNA‑15a‑5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/β‑catenin signaling pathway". Oncology Letters 21, no. 4 (2021): 310. https://doi.org/10.3892/ol.2021.12570
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