Open Access

Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway

  • Authors:
    • Jiahui Wei
    • Xuefeng Zhang
    • Huihao Pan
    • Song He
    • Bao Yuan
    • Qing Liu
    • Jiabao Zhang
    • Yu Ding
  • View Affiliations

  • Published online on: February 25, 2021     https://doi.org/10.3892/ol.2021.12593
  • Article Number: 332
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Eupafolin is a flavonoid extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti‑inflammatory, and anti‑tumor effects. However, its role in breast cancer remains unclear. The present study investigated the effects and underlying mechanism of action of Eupafolin using breast cancer cell lines. The effects of Eupafolin on breast cancer cell proliferation, migration, apoptosis and the cell cycle were determined. Cell viability and Transwell assays, reverse transcription‑quantitative PCR, flow cytometry and western blot analysis were used in this study. The data showed that the proliferation, migration and invasion ability of EO771 cells treated with Eupafolin was significantly decreased, and the apoptosis rate was increased compared with that of the control. The protein levels of Bax and cleaved caspase 3 increased, whereas that of Bcl‑2 decreased. In addition, Eupafolin treatment also caused the proliferation of breast cancer cells to be arrested at the G0/G1 phase. Furthermore, results from western blotting indicated that Eupafolin treatment decreased the protein levels of p‑PI3K, p‑Akt and p‑mTOR. Taken together, the present findings demonstrate that Eupafolin has a significant inhibitory effect on the proliferation of EO771 cells, inhibits cell migration and invasion, and promotes cell apoptosis, thereby causing G0/G1 phase arrest, at least partially through the PI3K/Akt/mTOR signaling pathway. Therefore, the findings provide novel insights regarding the use of Eupafolin for the treatment of breast cancer.
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April-2021
Volume 21 Issue 4

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Spandidos Publications style
Wei J, Zhang X, Pan H, He S, Yuan B, Liu Q, Zhang J and Ding Y: Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway. Oncol Lett 21: 332, 2021.
APA
Wei, J., Zhang, X., Pan, H., He, S., Yuan, B., Liu, Q. ... Ding, Y. (2021). Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway. Oncology Letters, 21, 332. https://doi.org/10.3892/ol.2021.12593
MLA
Wei, J., Zhang, X., Pan, H., He, S., Yuan, B., Liu, Q., Zhang, J., Ding, Y."Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway". Oncology Letters 21.4 (2021): 332.
Chicago
Wei, J., Zhang, X., Pan, H., He, S., Yuan, B., Liu, Q., Zhang, J., Ding, Y."Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway". Oncology Letters 21, no. 4 (2021): 332. https://doi.org/10.3892/ol.2021.12593