Open Access

Hydroxyl safflower yellow B combined with doxorubicin inhibits the proliferation of human breast cancer MCF‑7 cells

  • Authors:
    • Kehao Lin
    • Ze Qin
    • Chuanjun Qu
    • Xiaoyu Chen
    • Qingling Jiang
    • Minjing Li
    • Qiusheng Zheng
    • Defang Li
  • View Affiliations

  • Published online on: March 29, 2021     https://doi.org/10.3892/ol.2021.12687
  • Article Number: 426
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Doxorubicin (DOX) is currently the preferred chemotherapeutic agent for breast cancer, and hydroxyl safflower yellow B (HSYB) has a tumor growth‑inhibiting activity. The present study aimed to investigate the effects of HSYB combined with DOX on the proliferation of human breast cancer MCF‑7 cells and explore the underlying mechanism. MTT and cell colony formation assays revealed that the proliferation rate of MCF‑7 cells was signifiscantly decreased after HSYB and DOX treatment. Combined HSYB and DOX treatment significantly decreased the expression levels of BCL‑2 in MCF‑7 cells, while the expression levels of apoptosis‑associated proteins, including cleaved caspase‑9, BAX and cleaved caspase‑3, were markedly increased. Furthermore, flow cytometry and western blot analysis demonstrated that combined HSYB and DOX treatment stimulated an increase in intracellular reactive oxygen species and promoted the release of cytochrome c, leading to apoptosis. The current data suggested that the combination of HSYB and DOX may have marked antitumor activity.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Lin K, Qin Z, Qu C, Chen X, Jiang Q, Li M, Zheng Q and Li D: Hydroxyl safflower yellow B combined with doxorubicin inhibits the proliferation of human breast cancer MCF‑7 cells. Oncol Lett 21: 426, 2021
APA
Lin, K., Qin, Z., Qu, C., Chen, X., Jiang, Q., Li, M. ... Li, D. (2021). Hydroxyl safflower yellow B combined with doxorubicin inhibits the proliferation of human breast cancer MCF‑7 cells. Oncology Letters, 21, 426. https://doi.org/10.3892/ol.2021.12687
MLA
Lin, K., Qin, Z., Qu, C., Chen, X., Jiang, Q., Li, M., Zheng, Q., Li, D."Hydroxyl safflower yellow B combined with doxorubicin inhibits the proliferation of human breast cancer MCF‑7 cells". Oncology Letters 21.5 (2021): 426.
Chicago
Lin, K., Qin, Z., Qu, C., Chen, X., Jiang, Q., Li, M., Zheng, Q., Li, D."Hydroxyl safflower yellow B combined with doxorubicin inhibits the proliferation of human breast cancer MCF‑7 cells". Oncology Letters 21, no. 5 (2021): 426. https://doi.org/10.3892/ol.2021.12687