lncRNA LINC00473 promotes proliferation, migration, invasion and inhibition of apoptosis of non‑small cell lung cancer cells by acting as a sponge of miR‑497‑5p

Xu,Shao-Hua; Bo,Yu-Hong; Ma,Han-Chen; Zhang,Hua-Nan; Shao,Ming-Ju

doi:10.3892/ol.2021.12690

lncRNA LINC00473 promotes proliferation, migration, invasion and inhibition of apoptosis of non‑small cell lung cancer cells by acting as a sponge of miR‑497‑5p

Authors:
- Shao-Hua Xu
- Yu-Hong Bo
- Han-Chen Ma
- Hua-Nan Zhang
- Ming-Ju Shao
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Affiliations: Department of Respiration, The Second Hospital of Shandong University, Ji'nan, Shandong, 250000, P.R. China, Department of Respiration, Weihai Municipal Hospital, Weihai, Shandong 264200, P.R. China, Department of Emergency Medicine, The Second Hospital of Shandong University, Ji'nan, Shandong 250000, P.R. China
Published online on: March 30, 2021 https://doi.org/10.3892/ol.2021.12690
Article Number: 429
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lung cancer is the leading cause of cancer‑associated death worldwide and exhibits a poor prognosis. The present study aimed to determine the effect of long non‑coding (lnc)RNA‑LINC00473 on the development of non‑small cell lung cancer (NSCLC) cells by regulating the expression of microRNA (miR)‑497‑5p. Reverse transcription‑quantitative PCR was conducted to detect the level of LINC00473 and miR‑497‑5p. An MTT assay, flow cytometry and Transwell tests were performed to evaluate the proliferation, apoptosis, migration and invasion of NSCLC cells. Western blotting was performed to detect the expression of apoptosis‑ and migration‑related proteins. RNA immunoprecipitation and a luciferase reporter assay were performed to verify the regulatory relationship between lncRNA‑LINC00473 and miR‑497‑5p. LINC00473 expression was upregulated in lung cancer tissues and NSCLC cells (A549 and H1299) when compared with adjacent tissues or human bronchial epithelial cell lines and the 5‑year survival rate was lower in patients with high LINC00473 expression compared with in patients with low LINC00473 expression. A negative correlation between LINC00473 and miR‑497‑5p was observed in lung cancer tissues. Proliferation, migration and invasion as well as the related protein levels were increased in A549 and H1299 transfected with pcDNA3.1‑LINC00473, while the opposite results were obtained in A549 and H1299 transfected with small interfering (si)‑LINC00473. Notably, it was demonstrated that LINC00473 could bind directly with miR‑497‑5p and inhibit its expression. miR‑497‑5p inhibitors reversed the effect of si‑LINC00473. Furthermore, the present study demonstrated that LINC00473 promoted the malignant behaviour of NSCLC cells via regulating the ERK/p38 and MAPK signalling pathways and the expression of miR‑497‑5p.

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