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Article Open Access

MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma

  • Authors:
    • Yong Li
    • Dai Song
    • Ting An
    • Jie Liu
    • Qian Yang
    • Shaokui Nan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Sixth Medical Center of the PLA General Hospital, Beijing 100048, P.R. China, Community Health Service Center of South Railway Station, Chengdu, Sichuan 610042, P.R. China, Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 474
    |
    Published online on: April 15, 2021
       https://doi.org/10.3892/ol.2021.12735
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Abstract

Osteosarcoma is a malignant bone tumor that commonly occurs in young individuals. It accounts for 10% of solid tumors in those who are 15‑19 years old. MicroRNA (miRNA/miR) dysregulation serves a crucial role in the molecular mechanism of osteosarcoma. The present study reported a novel miRNA (miR‑1226‑3p) and investigated its function in osteosarcoma. miR‑1226‑3p mimics and miR‑1226‑3p antisense oligonucleotides were transfected into human osteosarcoma SaOS‑2 cells to alter miR‑1226‑3 expression, while the hFOB 1.19 cell line was used as the control. The apoptosis rate was analyzed using a dead cell apoptosis kit. TNF receptor‑associated factor 3 (TRAF3) protein expression was assayed by western blotting. The results of bioinformatics and clinical specimen analyses revealed that higher expression levels of miR‑1226‑3p were associated with lower survival rates. Additionally, the results of experiments on cultured cells revealed that miR‑1226‑3p promoted the proliferation of SaOS‑2 cells, while miR‑1226‑3p inhibition decreased cell proliferation and increased apoptosis. Furthermore, it was revealed that miR‑1226‑3p targeted TRAF3 in SaOS‑2 cells. In conclusion, the present study suggested that miR‑1226‑3p promoted the proliferation of osteosarcoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Song D, An T, Liu J, Yang Q and Nan S: MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncol Lett 21: 474, 2021.
APA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., & Nan, S. (2021). MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncology Letters, 21, 474. https://doi.org/10.3892/ol.2021.12735
MLA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21.6 (2021): 474.
Chicago
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21, no. 6 (2021): 474. https://doi.org/10.3892/ol.2021.12735
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Song D, An T, Liu J, Yang Q and Nan S: MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncol Lett 21: 474, 2021.
APA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., & Nan, S. (2021). MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncology Letters, 21, 474. https://doi.org/10.3892/ol.2021.12735
MLA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21.6 (2021): 474.
Chicago
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21, no. 6 (2021): 474. https://doi.org/10.3892/ol.2021.12735
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