Open Access

MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma

  • Authors:
    • Yong Li
    • Dai Song
    • Ting An
    • Jie Liu
    • Qian Yang
    • Shaokui Nan
  • View Affiliations

  • Published online on: April 15, 2021     https://doi.org/10.3892/ol.2021.12735
  • Article Number: 474
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteosarcoma is a malignant bone tumor that commonly occurs in young individuals. It accounts for 10% of solid tumors in those who are 15‑19 years old. MicroRNA (miRNA/miR) dysregulation serves a crucial role in the molecular mechanism of osteosarcoma. The present study reported a novel miRNA (miR‑1226‑3p) and investigated its function in osteosarcoma. miR‑1226‑3p mimics and miR‑1226‑3p antisense oligonucleotides were transfected into human osteosarcoma SaOS‑2 cells to alter miR‑1226‑3 expression, while the hFOB 1.19 cell line was used as the control. The apoptosis rate was analyzed using a dead cell apoptosis kit. TNF receptor‑associated factor 3 (TRAF3) protein expression was assayed by western blotting. The results of bioinformatics and clinical specimen analyses revealed that higher expression levels of miR‑1226‑3p were associated with lower survival rates. Additionally, the results of experiments on cultured cells revealed that miR‑1226‑3p promoted the proliferation of SaOS‑2 cells, while miR‑1226‑3p inhibition decreased cell proliferation and increased apoptosis. Furthermore, it was revealed that miR‑1226‑3p targeted TRAF3 in SaOS‑2 cells. In conclusion, the present study suggested that miR‑1226‑3p promoted the proliferation of osteosarcoma cells.
View Figures
View References

Related Articles

Journal Cover

June-2021
Volume 21 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Song D, An T, Liu J, Yang Q and Nan S: MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncol Lett 21: 474, 2021
APA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., & Nan, S. (2021). MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma. Oncology Letters, 21, 474. https://doi.org/10.3892/ol.2021.12735
MLA
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21.6 (2021): 474.
Chicago
Li, Y., Song, D., An, T., Liu, J., Yang, Q., Nan, S."MicroRNA‑1226‑3p has a tumor‑promoting role in osteosarcoma". Oncology Letters 21, no. 6 (2021): 474. https://doi.org/10.3892/ol.2021.12735