Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway

  • Authors:
    • Xige Chen
    • Juan Gao
    • Ning Liang
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China, Department of Dermatology, The Affiliated Changzhou No. 2 People's Hospital with Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China
  • Article Number: 495
    |
    Published online on: April 27, 2021
       https://doi.org/10.3892/ol.2021.12756
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Double homeobox A pseudogene 8 (DUXAP8) has been reported to regulate the growth of several types of cancers, such as breast cancer and ovarian cancer. However, its role in melanoma remains unclear. In the present study, the mechanism through which DUXAP8 regulates melanoma progression was explored. The expression levels of DUXAP8 were determined in 43 samples from patients with melanoma in different stages, as well as human epidermal melanocytes cells and malignant melanoma cell lines using reverse transcription‑quantitative PCR (RT‑qPCR). The prognosis of patients was analyzed using the Kaplan‑Meier method. The relationship between lncRNA DUXAP8 expression and microRNA (miR)‑3182 or nuclear protein 1 transcriptional regulator (NUPR1) levels was analyzed using Pearson's correlation. Luciferase reporter and RNA pull‑down were used to examine the interactions between these molecules. Proliferation was assessed using Cell Counting‑Kit‑8. Transwell assays were used to examine cell migration and invasion. lncRNA DUXAP8 was upregulated in melanoma tissue and cells compared with normal tissues and cells. The levels of DUXAP8 inversely correlated with survival time of patients with melanoma. Knockdown of lncRNA DUXAP8 inhibited proliferation, migration and invasion of melanoma cells. lncRNA DUXAP8 targeted miR‑3182, while miR‑3182 targeted NUPR1. The overexpression of NUPR1 reversed the effects of DUXAP8 knockdown or miR‑3182 mimic on melanoma progression. In conclusion, lncRNA DUXAP8 downregulation inhibits the development of melanoma by regulating the miR‑3182/NUPR1 axis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Fodstad O, Aass N and Pihl A: An inverse relationship between the growth rate of human melanoma xenografts and their response to some cytostatic drugs. Br J Cancer. 41:829–831. 1980. View Article : Google Scholar : PubMed/NCBI

2 

Farahmand AM, Ehsani AH, Mirzaei M, Mohsenian M and Ghanadan A: Patients' characteristics, histopathological findings, and tumor stage in different types of malignant melanoma: A retrospective multicenter study. Acta Med Iran. 55:316–323. 2017.PubMed/NCBI

3 

Hu DN, Yu GP and McCormick SA: Population-based incidence of vulvar and vaginal melanoma in various races and ethnic groups with comparisons to other site-specific melanomas. Melanoma Res. 20:153–158. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Del Vecchio M: AACR update on 5-year survival rates, effcacy and long-term safety in previously treated advanced/metastatic melanoma patients receiving mono-immunotherapy with nivolumab. Recenti Prog Med. 107:414–417. 2016.(In Italian). PubMed/NCBI

5 

Morton DL, Davtyan DG, Wanek LA, Foshag LJ and Cochran AJ: Multivariate analysis of the relationship between survival and the microstage of primary melanoma by Clark level and Breslow thickness. Cancer. 71:3737–3743. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Pfahlberg A, Kölmel KF and Gefeller O; Febim Study Group, : Timing of excessive ultraviolet radiation and melanoma: Epidemiology does not support the existence of a critical period of high susceptibility to solar ultraviolet radiation-induced melanoma. Br J Dermatol. 144:471–475. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Thoelke A, Willrodt S, Hauschild A and Schadendorf D: Primary extracutaneous malignant melanoma: A comprehensive review with emphasis on treatment. Onkologie. 27:492–499. 2004.PubMed/NCBI

8 

Gasparini G: Tamoxifen and chemotherapy in the treatment of metastatic melanoma: Are there other possible mechanisms explaining their potentiation? J Clin Oncol. 12:1994–1996. 1994. View Article : Google Scholar : PubMed/NCBI

9 

Wang X, Moschos SJ and Becker D: Functional analysis and molecular targeting of aurora kinases a and B in advanced melanoma. Genes Cancer. 1:952–963. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Chi Z, Li S, Sheng X, Si L and Guo J: Clinical presentation, histology, and prognoses of malignant melanoma in ethnic Chinese: A study of 522 consecutive cases. BMC Cancer. 11:852011. View Article : Google Scholar : PubMed/NCBI

11 

Barth A, Wanek L and Morton DL: Prognostic factors in 1,521 melanoma patients with distant metastases. J Am Coll Surg. 181:193–201. 1995.PubMed/NCBI

12 

Ribero S, Glass D and Bataille V: Genetic epidemiology of melanoma. Eur J Dermatol. 26:335–339. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH and Novina CD: The lncRNA SLNCR1 mediates melanoma invasion through a conserved SRA1-like region. Cell Rep. 15:2025–2037. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Gong J, Zhang H, He L, Wang L and Wang J: Increased expression of long non-coding RNA BCAR4 is predictive of poor prognosis in patients with non-small cell lung cancer. Tohoku J Exp Med. 241:29–34. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Wei F, Yang L, Jiang D, Pan M, Tang G, Huang M and Zhang J: Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR-422a/PDK2 axis. Cancer Med. 9:2480–2490. 2020. View Article : Google Scholar : PubMed/NCBI

16 

Ji X, Tao R, Sun LY, Xu XL and Ling W: Down-regulation of long non-coding RNA DUXAP8 suppresses proliferation, metastasis and EMT by modulating miR-498 through TRIM44-mediated AKT/mTOR pathway in non-small-cell lung cancer. Eur Rev Med Pharmacol Sci. 24:3152–3165. 2020.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Sun M, Nie FQ, Zang C, Wang Y, Hou J, Wei C, Li W, He X and Lu KH: The pseudogene DUXAP8 promotes non-small-cell lung cancer cell proliferation and invasion by epigenetically silencing EGR1 and RHOB. Mol Ther. 25:739–751. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Ma HW, Xie M, Sun M, Chen TY, Jin RR, Ma TS, Chen QN, Zhang EB, He XZ, De W and Zhang ZH: The pseudogene derived long noncoding RNA DUXAP8 promotes gastric cancer cell proliferation and migration via epigenetically silencing PLEKHO1 expression. Oncotarget. 8:52211–52224. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Xu X, Xu Y, Shi C, Wang B, Yu X, Zou Y and Hu T: A genome-wide comprehensively analyses of long noncoding RNA profiling and metastasis associated lncRNAs in renal cell carcinoma. Oncotarget. 8:87773–87781. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Goundiam O, Nagel MD and Vayssade M: Akt and RhoA inhibition promotes anoikis of aggregated B16F10 melanoma cells. Cell Biol Int. 36:311–319. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Delmas A, Cherier J, Medale-Giamarchi C, Pradines A and Favre G: Abstract 3401: Vemurafenib-induced RhoB expression leads to melanoma cell resistance. Cancer Res. 73 (Suppl 8):S34012013.

23 

Eide A: The role of the sub-commission on promotion and protection of human rights and its working groups in the prevention of conflicts. Int J Minor Group Rights. 8:25–29. 2001. View Article : Google Scholar

24 

Gao K, Zhou H, Zhang L, Lee JW, Zhou Q, Hu S, Farrell J, Eibl G and Wong D: Abstract #314: Induction of systemic disease-specific salivary biomarker profiles in mouse models of melanoma and non-small cell lung cancer. Cancer Res. 69 (Suppl 9):S3142009.

25 

Razaviyan J, Hadavi R, Tavakoli R, Kamani F, Paknejad M and Mohammadi-Yeganeh S: Expression of miRNAs targeting mTOR and S6K1 genes of mTOR signaling pathway including miR-96, miR-557, and miR-3182 in triple-negative breast cancer. Appl Biochem Biotechnol. 186:1074–1089. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Zhu KP, Ma XL and Zhang CL: LncRNA ODRUL contributes to osteosarcoma progression through the miR-3182/MMP2 axis. Mol Ther. 25:2383–2393. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Xue M, Shi D, Xu G and Wang W: The long noncoding RNA linc00858 promotes progress of lung cancer through miR-3182/MMP2 axis. Artif Cells Nanomed Biotechnol. 47:2091–2097. 2019. View Article : Google Scholar : PubMed/NCBI

28 

Zhou MY, Lan GP, Si YF and Mo XG: Expression patterns and significances of distant metastasis and prognosis of MiR-3182 innasopharygeal carcinoma. Chin J New Clin Med. 5:369–372. 2016.

29 

Zhao G, Han X, Zheng S, Li Z, Sha Y, Ni J, Sun Z, Qiao S and Song Z: Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells. Oncol Rep. 35:1065–1074. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Guo X, Wang W, Hu J, Feng K, Pan Y, Zhang L and Feng Y: Lentivirus-mediated RNAi knockdown of NUPR1 inhibits human nonsmall cell lung cancer growth in vitro and in vivo. Anat Rec (Hoboken). 295:2114–2121. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Cano CE, Hamidi T, Garcia MN, Grasso D, Loncle C, Garcia S, Calvo E, Lomberk G, Dusetti N, Bartholin L, et al: Genetic inactivation of Nupr1 acts as a dominant suppressor event in a two-hit model of pancreatic carcinogenesis. Gut. 63:984–995. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Emma MR, Iovanna JL, Bachvarov D, Puleio R, Loria GR, Augello G, Candido S, Libra M, Gulino A, Cancila V, et al: NUPR1, a new target in liver cancer: Implication in controlling cell growth, migration, invasion and sorafenib resistance. Cell Death Dis. 7:e22692016. View Article : Google Scholar : PubMed/NCBI

33 

Boregowda RK, Olabisi OO, Abushahba W, Jeong BS, Haenssen KK, Chen W, Chekmareva M, Lasfar A, Foran DJ, Goydos JS and Cohen-Solal KA: RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion. Cancer Lett. 348:61–70. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Wu H, Goel V and Haluska FG: PTEN signaling pathways in melanoma. Oncogene. 22:3113–3122. 2003. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen X, Gao J and Liang N: DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway. Oncol Lett 22: 495, 2021.
APA
Chen, X., Gao, J., & Liang, N. (2021). DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway. Oncology Letters, 22, 495. https://doi.org/10.3892/ol.2021.12756
MLA
Chen, X., Gao, J., Liang, N."DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway". Oncology Letters 22.1 (2021): 495.
Chicago
Chen, X., Gao, J., Liang, N."DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway". Oncology Letters 22, no. 1 (2021): 495. https://doi.org/10.3892/ol.2021.12756
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Gao J and Liang N: DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway. Oncol Lett 22: 495, 2021.
APA
Chen, X., Gao, J., & Liang, N. (2021). DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway. Oncology Letters, 22, 495. https://doi.org/10.3892/ol.2021.12756
MLA
Chen, X., Gao, J., Liang, N."DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway". Oncology Letters 22.1 (2021): 495.
Chicago
Chen, X., Gao, J., Liang, N."DUXAP8 knockdown inhibits the development of melanoma by regulating the miR‑3182/NUPR1 pathway". Oncology Letters 22, no. 1 (2021): 495. https://doi.org/10.3892/ol.2021.12756
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team