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Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma

  • Authors:
    • Jinhua Zhang
    • Linhui Li
    • Lilin Luo
    • Xuantao Yang
    • Juanjuan Zhang
    • Yuxin Xie
    • Rui Liang
    • Wanpu Wang
    • Shuaiyao Lu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, P.R. China, Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan 650118, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 506
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    Published online on: May 2, 2021
       https://doi.org/10.3892/ol.2021.12767
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Abstract

Accumulating evidence has indicated that a group of novel molecules, known as transfer RNA (tRNA)‑derived fragments (tRFs) and tRNA halves (tiRNAs), which are derived from tRNAs, serve an essential role in numerous types of human disease, in particular solid tumors. However, to the best of our knowledge, the underlying mechanisms of the effect of tRFs and tiRNAs in lung adenocarcinoma have not been reported. The present study aimed to determine the differential expression levels of tRFs and tiRNAs in lung adenocarcinoma and adjacent tissues using a NextSeq system, and further investigated their potential target genes via bioinformatics analysis. Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology functional term enrichment analyses were performed to investigate the function of these target genes in the occurrence and development of lung adenocarcinoma. In patients with lung adenocarcinoma, 338 types of tRFs and tiRNAs were detected via sequencing, 284 of which were not previously reported in the tRF database. Compared with the adjacent tissues, 17 types of tRFs and tiRNAs comprising 34 subtypes were found to be abnormally expressed in lung adenocarcinoma tissues, 20 of which were upregulated and 14 downregulated. Reverse transcription‑quantitative PCR verification revealed that the expression levels of tiRNA‑Lys‑CTT‑002, tRF‑Val‑CAC‑010 and tRF‑Val‑CAC‑011 were significantly upregulated, while those of tRF‑Ser‑TGA‑005 were downregulated in lung adenocarcinoma tissues. Bioinformatics analysis identified that tRF‑Ser‑TGA‑005 participated in the ‘cellular response to transforming growth factor β stimulus’ and tRF‑Val‑CAC‑010 and tRF‑Val‑CAC‑011 participated in the ‘Hedgehog signaling pathway’. In conclusion, the results of the present study suggested that tRFs and tiRNAs may be closely associated with the pathogenesis and development of lung adenocarcinoma, providing a novel insight for further studies into lung adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Li L, Luo L, Yang X, Zhang J, Xie Y, Liang R, Wang W and Lu S: Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma. Oncol Lett 22: 506, 2021.
APA
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y. ... Lu, S. (2021). Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma. Oncology Letters, 22, 506. https://doi.org/10.3892/ol.2021.12767
MLA
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y., Liang, R., Wang, W., Lu, S."Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma". Oncology Letters 22.1 (2021): 506.
Chicago
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y., Liang, R., Wang, W., Lu, S."Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma". Oncology Letters 22, no. 1 (2021): 506. https://doi.org/10.3892/ol.2021.12767
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Li L, Luo L, Yang X, Zhang J, Xie Y, Liang R, Wang W and Lu S: Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma. Oncol Lett 22: 506, 2021.
APA
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y. ... Lu, S. (2021). Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma. Oncology Letters, 22, 506. https://doi.org/10.3892/ol.2021.12767
MLA
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y., Liang, R., Wang, W., Lu, S."Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma". Oncology Letters 22.1 (2021): 506.
Chicago
Zhang, J., Li, L., Luo, L., Yang, X., Zhang, J., Xie, Y., Liang, R., Wang, W., Lu, S."Screening and potential role of tRFs and tiRNAs derived from tRNAs in the carcinogenesis and development of lung adenocarcinoma". Oncology Letters 22, no. 1 (2021): 506. https://doi.org/10.3892/ol.2021.12767
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