GFI‑1 overexpression promotes cell proliferation and apoptosis resistance in mycosis fungoides by repressing Bax and P21
- Xiaoguang Gu
- Yimeng Wang
- Chunlei Zhang
- Yongsheng Liu
Affiliations: Department of Dermatology and Venerology, Aviation General Hospital, Beijing 100012, P.R. China, Department of Dermatology and Venerology, Peking University Third Hospital, Beijing 100191, P.R. China
- Published online on: May 11, 2021 https://doi.org/10.3892/ol.2021.12782
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Mycosis fungoides (MF) is the most common type of cutaneous T‑cell lymphoma. The majority of patients with advanced stage MF are resistant to conventional chemotherapy and thus have a poor prognosis. The transcriptional repressor growth factor independence‑1 (GFI‑1) serves an important role in the development of T‑cells. The results of the present study demonstrated that the expression of GFI‑1 at different clinical stages of MF was significantly higher compared with benign inflammatory dermatoses, and there was a significant association with disease progression. Gene knockdown of GFI‑1 results in the inhibition of Hut‑78 cell proliferation and clone formation in vitro, cell cycle arrest and spontaneous apoptosis, upregulation of cell cycle‑related P21, as well as the apoptosis‑related proteins Bax and Caspase‑3, and downregulation of CDK2. Using luciferase assays, and mutational analysis, it was demonstrated that GFI‑1 directly regulated the transcription of P21. The results of the present study highlighted a potential molecular therapeutic approach for the treatment of advanced MF.