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miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines

  • Authors:
    • Sixing Chen
    • Yu Chen
    • Yao Wen
    • Wanwan Cai
    • Ping Zhu
    • Wuzhou Yuan
    • Yongqing Li
    • Xiongwei Fan
    • Yongqi Wan
    • Fang Li
    • Jian Zhuang
    • Zhigang Jiang
    • Xiushan Wu
    • Yuequn Wang
  • View Affiliations / Copyright

    Affiliations: The Center for Heart Development, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 532
    |
    Published online on: May 17, 2021
       https://doi.org/10.3892/ol.2021.12793
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Abstract

Required for meiotic nuclear division 5 homolog A (RMND5A) functions as an E3 ubiquitin ligase. To date, few studies have investigated the role of RMND5A in cancer. In the present study, the expression levels of RMND5A in multiple types of cancer were analyzed using the Gene Expression Profiling Interactive Analysis platform. The results revealed that RMND5A was highly expressed and associated with overall survival in patients with pancreatic adenocarcinoma (PAAD). A wound‑healing assay revealed that RMND5A overexpression significantly increased cell migration in the PAAD cell lines AsPC‑1 and PANC‑1. In silico analysis predicted that RMND5A was a potential target of microRNA(miR)‑590‑5p. Further in vitro experiments demonstrated that overexpression of miR‑590‑5p downregulated the expression levels of RMND5A and decreased the migratory ability of the AsPC‑1 and PANC‑1 cell lines. In addition, overexpression of miR‑590‑5p attenuated the promoting effects of RMND5A on the migration of AsPC‑1 and PANC‑1 cells. The results of the present study may further elucidate the mechanisms underlying PAAD progression and provide novel targets for the treatment of PAAD.
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1 

Pfirrmann T, Villavicencio-Lorini P, Subudhi AK, Menssen R, Wolf DH and Hollemann T: RMND5 from Xenopus laevis is an E3 ubiquitin-ligase and functions in early embryonic forebrain development. PLoS One. 10:e01203422015. View Article : Google Scholar : PubMed/NCBI

2 

Maitland MER, Onea G, Chiasson CA, Wang X, Ma J, Moor SE, Barber KR, Lajoie GA, Shaw GS and Schild-Poulter C: The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation. Sci Rep. 9:98642019. View Article : Google Scholar : PubMed/NCBI

3 

van Wijk SJ, de Vries SJ, Kemmeren P, Huang A, Boelens R, Bonvin AM and Timmers HT: A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5:2952009. View Article : Google Scholar : PubMed/NCBI

4 

McTavish CJ, Berube-Janzen W, Wang X, Maitland MER, Salemi LM, Hess DA and Schild-Poulter C: Regulation of c-Raf Stability through the CTLH Complex. Int J Mol Sci. 20:9342019. View Article : Google Scholar : PubMed/NCBI

5 

Huffman N, Palmieri D and Coppola V: The CTLH complex in cancer cell plasticity. J Oncol. 2019:42167502019. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Chen Y, Qin X, Wen J, Ding H, Xia W, Li S, Su X, Wang W, Li H, et al: miR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability. Nucleic Acids Res. 42:458–474. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Liu H and Ye H: Screening of the prognostic targets for breast cancer based co-expression modules analysis. Mol Med Rep. 16:4038–4044. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Djuranovic S, Nahvi A and Green R: A parsimonious model for gene regulation by miRNAs. Science. 331:550–553. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Filipowicz W, Bhattacharyya SN and Sonenberg N: Mechanisms of post-transcriptional regulation by microRNAs: Are the answers in sight? Nat Rev Genet. 9:102–114. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Kusenda B, Mraz M, Mayer J and Pospisilova S: MicroRNA biogenesis, functionality and cancer relevance. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 150:205–215. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Brennecke J, Hipfner DR, Stark A, Russell RB and Cohen SM: Bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell. 113:25–36. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Chen CZ, Li L, Lodish HF and Bartel DP: MicroRNAs modulate hematopoietic lineage differentiation. Science. 303:83–86. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Iorio MV and Croce CM: MicroRNA dysregulation in cancer: Diagnostics, monitoring and therapeutics. A comprehensive review. EMBO Mol Med. 4:143–159. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, Bartel DP, Linsley PS and Johnson JM: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 433:769–773. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Chang JT, Wang F, Chapin W and Huang RS: Identification of MicroRNAs as breast cancer prognosis markers through the cancer genome atlas. PLoS One. 11:e01682842016. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Zhu K, Zhang Z, Zhang H, Wang Z and Wang F: miR-142-3p targeting NUCKS1 inhibits proliferation and invasion of pancreatic cancer cells. Artif Cells Nanomed Biotechnol. 48:415–424. 2020. View Article : Google Scholar : PubMed/NCBI

19 

Yamauchi A, Yamamura M, Katase N, Itadani M, Okada N, Kobiki K, Nakamura M, Yamaguchi Y and Kuribayashi F: Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device. BMC Cancer. 17:2342017. View Article : Google Scholar : PubMed/NCBI

20 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Roussos ET, Condeelis JS and Patsialou A: Chemotaxis in cancer. Nat Rev Cancer. 11:573–587. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Lambert AW, Pattabiraman DR and Weinberg RA: Emerging biological principles of metastasis. Cell. 168:670–691. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Xu BB, Gu ZF, Ma M, Wang JY and Wang HN: MicroRNA-590-5p suppresses the proliferation and invasion of non-small cell lung cancer by regulating GAB1. Eur Rev Med Pharmacol Sci. 22:5954–5963. 2018.PubMed/NCBI

24 

Xu Y, Han T, Zhu Y and Chen Q: miR-590-5P inhibits the progression of tongue squamous cell carcinoma by targeting FasL. Int J Clin Exp Pathol. 10:11880–11887. 2017.PubMed/NCBI

25 

Yan M, Ye L, Feng X, Shi R, Sun Z, Li Z and Liu T: MicroRNA-590-3p inhibits invasion and metastasis in triple-negative breast cancer by targeting Slug. Am J Cancer Res. 10:965–974. 2020.PubMed/NCBI

26 

Gao J, Yu SR, Yuan Y, Zhang LL, Lu JW, Feng JF and Hu SN: MicroRNA-590-5p functions as a tumor suppressor in breast cancer conferring inhibitory effects on cell migration, invasion, and epithelial-mesenchymal transition by downregulating the Wnt-β-catenin signaling pathway. J Cell Physiol. 234:1827–1841. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Shan X, Miao Y, Fan R, Qian H, Chen P, Liu H, Yan X, Li J and Zhou F: miR-590-5P inhibits growth of HepG2 cells via decrease of S100A10 expression and Inhibition of the Wnt pathway. Int J Mol Sci. 14:8556–8569. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Chen M, Wu L, Tu J, Zhao Z, Fan X, Mao J, Weng Q, Wu X, Huang L, Xu M and Ji J: miR-590-5p suppresses hepatocellular carcinoma chemoresistance by targeting YAP1 expression. EBioMedicine. 35:142–154. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Mou K, Liu B, Ding M, Mu X, Han D, Zhou Y and Wang LJ: lncRNA-ATB functions as a competing endogenous RNA to promote YAP1 by sponging miR-590-5p in malignant melanoma. Int J Oncol. 53:1094–1104. 2018.PubMed/NCBI

30 

Chu Y, Ouyang Y, Wang F, Zheng A, Bai L, Han L, Chen Y and Wang H: MicroRNA-590 promotes cervical cancer cell growth and invasion by targeting CHL1. J Cell Biochem. 115:847–853. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Xiao X, Tang C, Xiao S, Fu C and Yu P: Enhancement of proliferation and invasion by MicroRNA-590-5p via targeting PBRM1 in clear cell renal carcinoma cells. Oncol Res. 20:537–544. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Wang L, Wei WQ, Wu ZY and Wang GC: MicroRNA-590-5p regulates cell viability, apoptosis, migration and invasion of renal cell carcinoma cell lines through targeting ARHGAP24. Mol Biosyst. 13:2564–2573. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Zhu M, Zhou X, Zhang J and Zhang Y: MicroRNA-590-5p promotes cell survival in endometrioid endometrial cancer by suppressing tumor suppressor PTEN. Int J Clin Exp Pathol. 10:7836–7846. 2017.PubMed/NCBI

34 

Jiang X, Xiang G, Wang Y, Zhang L, Yang X, Cao L, Peng H, Xue P and Chen D: MicroRNA-590-5p regulates proliferation and invasion in human hepatocellular carcinoma cells by targeting TGF-β RII. Mol Cells. 33:545–551. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Yang X and Wu X: miRNA expression profile of vulvar squamous cell carcinoma and identification of the oncogenic role of miR-590-5p. Oncol Rep. 35:398–408. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen S, Chen Y, Wen Y, Cai W, Zhu P, Yuan W, Li Y, Fan X, Wan Y, Li F, Li F, et al: miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines. Oncol Lett 22: 532, 2021.
APA
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W. ... Wang, Y. (2021). miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines. Oncology Letters, 22, 532. https://doi.org/10.3892/ol.2021.12793
MLA
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W., Li, Y., Fan, X., Wan, Y., Li, F., Zhuang, J., Jiang, Z., Wu, X., Wang, Y."miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines". Oncology Letters 22.1 (2021): 532.
Chicago
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W., Li, Y., Fan, X., Wan, Y., Li, F., Zhuang, J., Jiang, Z., Wu, X., Wang, Y."miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines". Oncology Letters 22, no. 1 (2021): 532. https://doi.org/10.3892/ol.2021.12793
Copy and paste a formatted citation
x
Spandidos Publications style
Chen S, Chen Y, Wen Y, Cai W, Zhu P, Yuan W, Li Y, Fan X, Wan Y, Li F, Li F, et al: miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines. Oncol Lett 22: 532, 2021.
APA
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W. ... Wang, Y. (2021). miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines. Oncology Letters, 22, 532. https://doi.org/10.3892/ol.2021.12793
MLA
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W., Li, Y., Fan, X., Wan, Y., Li, F., Zhuang, J., Jiang, Z., Wu, X., Wang, Y."miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines". Oncology Letters 22.1 (2021): 532.
Chicago
Chen, S., Chen, Y., Wen, Y., Cai, W., Zhu, P., Yuan, W., Li, Y., Fan, X., Wan, Y., Li, F., Zhuang, J., Jiang, Z., Wu, X., Wang, Y."miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines". Oncology Letters 22, no. 1 (2021): 532. https://doi.org/10.3892/ol.2021.12793
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