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MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2

  • Authors:
    • Xiurong Lu
    • Xiao Song
    • Xiaohui Hao
    • Xiaoyu Liu
    • Xianyu Zhang
    • Na Yuan
    • Huan Ma
    • Zhilin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 539
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    Published online on: May 19, 2021
       https://doi.org/10.3892/ol.2021.12800
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Abstract

The present study examined the effect of microRNA (miRNA/miR)‑186‑3p and its target gene, minichromosome maintenance complex component 2 (MCM2), on cervical cancer. Cervical cancer tissues and corresponding normal tissues were collected from 48 patients and bioinformatics analysis was performed to identify the differentially expressed genes in cervical cancer. TargetScan and TarBase were used to identify miRNAs, and reverse transcription‑quantitative PCR was conducted to detect and evaluate mRNA expression levels. Additionally, MTT and 5‑bromo‑2‑deoxyuridine assays were performed to examine cell proliferation. Cell adhesion, cell cycle distribution and apoptosis were assessed using cell adhesion, flow cytometry and caspase‑3/7 activity assays, respectively. The results revealed that miR‑186‑3p expression was downregulated in cervical cancer tissues and cells, and it negatively regulated MCM2 expression by directly targeting its 3' untranslated region in cervical cancer. Furthermore, MCM2 facilitated cell proliferation and inhibited cell apoptosis, which were reversed by upregulation of miR‑186‑3p expression. Collectively, the present study suggested that MCM2 and its negative regulator, miR‑186‑3p, regulate cervical cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Lu X, Song X, Hao X, Liu X, Zhang X, Yuan N, Ma H and Zhang Z: MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2. Oncol Lett 22: 539, 2021.
APA
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N. ... Zhang, Z. (2021). MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2. Oncology Letters, 22, 539. https://doi.org/10.3892/ol.2021.12800
MLA
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N., Ma, H., Zhang, Z."MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2". Oncology Letters 22.1 (2021): 539.
Chicago
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N., Ma, H., Zhang, Z."MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2". Oncology Letters 22, no. 1 (2021): 539. https://doi.org/10.3892/ol.2021.12800
Copy and paste a formatted citation
x
Spandidos Publications style
Lu X, Song X, Hao X, Liu X, Zhang X, Yuan N, Ma H and Zhang Z: MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2. Oncol Lett 22: 539, 2021.
APA
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N. ... Zhang, Z. (2021). MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2. Oncology Letters, 22, 539. https://doi.org/10.3892/ol.2021.12800
MLA
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N., Ma, H., Zhang, Z."MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2". Oncology Letters 22.1 (2021): 539.
Chicago
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N., Ma, H., Zhang, Z."MicroRNA‑186‑3p attenuates tumorigenesis of cervical cancer by targeting MCM2". Oncology Letters 22, no. 1 (2021): 539. https://doi.org/10.3892/ol.2021.12800
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