Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer

  • Authors:
    • Chi He
    • Yi Fu
    • Yan Chen
    • Xiquan Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shenyang Anorectal Hospital, Shenyang, Liaoning 110054, P.R. China, Department of General Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116000, P.R. China, Department of General Surgery, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163000, P.R. China
  • Article Number: 577
    |
    Published online on: June 2, 2021
       https://doi.org/10.3892/ol.2021.12838
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Colorectal cancer (CRC) is the third most common cancer worldwide. Long non‑coding RNA (lncRNA) small nucleolar RNA host gene 8 (SNHG8) acts as an oncogene in different types of cancer, including prostate, breast and ovarian cancer. SNHG8 promotes the tumorigenesis of CRC; however, its underlying molecular mechanism remains unclear. The present study aimed to explore the mechanism of SNHG8 on CRC development via various assays, including western blot, pull‑down, PCR and immunofluorescence assays. The results of the present study demonstrated that SNHG8 expression was substantially upregulated in primary tumor tissues from The Cancer Genome Atlas dataset. Western blot and immunofluorescence analyses demonstrated that SNHG8 facilitated cell proliferation and autophagy in CRC cells. Notably, the function of SNHG8 in enhancing autophagy was dependent on autophagy‑related gene 7 (ATG7). In addition, western blot analysis indicated that the effect of SNHG8 on autophagy in CRC cells was dependent on the miR‑588/ATG7 axis. Taken together, the results of the present study suggest that SNHG8 promotes autophagy in CRC cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Liu A and Liu L: Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis of colon cancer cells via miR-143/bcl-2 axis. Am J Transl Res. 11:5240–5248. 2019.PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A and Jemal A: Colorectal cancer statistics, 2017. CA Cancer J Clin. 67:177–193. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Li X, Zhou Y, Li Y, Yang L, Ma Y, Peng X, Yang S, Liu J and Li H: Autophagy: A novel mechanism of chemoresistance in cancers. Biomed Pharmacother. 119:1094152019. View Article : Google Scholar : PubMed/NCBI

5 

Li C, Lei B, Huang S, Zheng M, Liu Z, Li Z and Deng Y: H19 derived microRNA-675 regulates cell proliferation and migration through CDK6 in glioma. Am J Transl Res. 7:1747–1764. 2015.PubMed/NCBI

6 

Liu J, Tian W, Zhang W, Jia Y, Yang X, Wang Y and Zhang J: MicroRNA-142-3p/MALAT1 inhibits lung cancer progression through repressing β-catenin expression. Biomed Pharmacother. 114:1088472019. View Article : Google Scholar : PubMed/NCBI

7 

Amaravadi R, Kimmelman AC and White E: Recent insights into the function of autophagy in cancer. Genes Dev. 30:1913–1930. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Luo M, Li Z, Wang W, Zeng Y, Liu Z and Qiu J: Long non-coding RNA H19 increases bladder cancer metastasis by associating with EZH2 and inhibiting E-cadherin expression. Cancer Lett. 333:213–221. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Ma H, Yuan L, Li W, Xu K and Yang L: The lncRNA H19/miR-193a-3p axis modifies the radio-resistance and chemotherapeutic tolerance of hepatocellular carcinoma cells by targeting PSEN1. J Cell Biochem. 119:8325–8335. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Tang B, Bao N, He G and Wang J: Long noncoding RNA HOTAIR regulates autophagy via the miR-20b-5p/ATG7 axis in hepatic ischemia/reperfusion injury. Gene. 686:56–62. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Si Y, Yang Z, Ge Q, Yu L, Yao M, Sun X, Ren Z and Ding C: Long non-coding RNA Malat1 activated autophagy, hence promoting cell proliferation and inhibiting apoptosis by sponging miR-101 in colorectal cancer. Cell Mol Biol Lett. 24:502019. View Article : Google Scholar : PubMed/NCBI

12 

Zhang P, Li S, Chen Z, Lu Y and Zhang H: lncRNA SNHG8 promotes proliferation and invasion of gastric cancer cells by targeting the miR-491/PDGFRA axis. Hum Cell. 33:123–130. 2020. View Article : Google Scholar : PubMed/NCBI

13 

Fan D, Qiu B, Yang XJ, Tang HL, Peng SJ, Yang P, Dong YM, Yang L, Bao GQ and Zhao HD: lncRNA SNHG8 promotes cell migration and invasion in breast cancer cell through miR-634/ZBTB20 axis. Eur Rev Med Pharmacol Sci. 24:11639–11649. 2020.PubMed/NCBI

14 

Miao W, Lu T, Liu X, Yin W and Zhang H: lncRNA SNHG8 induces ovarian carcinoma cells cellular process and stemness through Wnt/β-catenin pathway. Cancer Biomark. 28:459–471. 2020. View Article : Google Scholar : PubMed/NCBI

15 

Ying SY, Chang DC and Lin SL: The microRNA (miRNA): Overview of the RNA genes that modulate gene function. Mol Biotechnol. 38:257–268. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Shukla GC, Singh J and Barik S: MicroRNAs: Processing, maturation, target recognition and regulatory functions. Mol Cell Pharmacol. 3:83–92. 2011.PubMed/NCBI

17 

Qian L, Lin L, Du Y, Hao X, Zhao Y and Liu X: MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Mol Med Rep. 14:3021–3028. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Yu M, Zhang X, Li H, Zhang P and Dong W: MicroRNA-588 is downregulated and may have prognostic and functional roles in human breast cancer. Med Sci Monit. 23:5690–5696. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Kohlhapp FJ, Mitra AK, Lengyel E and Peter ME: MicroRNAs as mediators and communicators between cancer cells and the tumor microenvironment. Oncogene. 34:5857–5868. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, et al: Short tandem repeat profiling provides an international reference standard for human cell lines. Proc Natl Acad Sci USA. 98:8012–8017. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Li HR, Shagisultanova EI, Yamashita K, Piao Z, Perucho M and Malkhosyan SR: Hypersensitivity of tumor cell lines with microsatellite instability to DNA double strand break producing chemotherapeutic agent bleomycin. Cancer Res. 64:4760–4767. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Mukhopadhyay S, Biancur DE, Parker SJ, Yamamoto K, Banh RS, Paulo JA, Mancias JD and Kimmelman AC: Autophagy is required for proper cysteine homeostasis in pancreatic cancer through regulation of SLC7A11. Proc Natl Acad Sci USA. 118:e20214751182021. View Article : Google Scholar : PubMed/NCBI

24 

Xu JL, Yuan L, Tang YC, Xu ZY, Xu HD, Cheng XD and Qin JJ: The role of autophagy in gastric cancer chemoresistance: Friend or foe? Front Cell Dev Biol. 8:6214282020. View Article : Google Scholar : PubMed/NCBI

25 

Yun CW, Jeon J, Go G, Lee JH and Lee SH: The dual role of autophagy in cancer development and a therapeutic strategy for cancer by targeting autophagy. Int J Mol Sci. 22:1792020. View Article : Google Scholar : PubMed/NCBI

26 

Shi Z, Zhang H, Jie S, Yang X, Huang Q, Mao Y and Zhang Y: Long non-coding RNA SNHG8 promotes prostate cancer progression through repressing miR-384 and up-regulating HOXB7. J Gene Med. e33092021.PubMed/NCBI

27 

Yu X, Zhu L, Liu J, Xie M, Chen J and Li J: Emerging role of immunotherapy for colorectal cancer with liver metastasis. Onco Targets Ther. 13:11645–11658. 2020. View Article : Google Scholar : PubMed/NCBI

28 

Jin KT, Tao XH, Fan YB and Wang SB: Crosstalk between oncolytic viruses and autophagy in cancer therapy. Biomed Pharmacother. 134:1109322021. View Article : Google Scholar : PubMed/NCBI

29 

Yan X, Hu Z, Feng Y, Hu X, Yuan J, Zhao SD, Zhang Y, Yang L, Shan W, He Q, et al: Comprehensive genomic characterization of long Non-coding RNAs across human cancers. Cancer Cell. 28:529–540. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Li CF, Li YC, Wang Y and Sun LB: The effect of lncRNA H19/miR-194-5p axis on the epithelial-mesenchymal transition of colorectal adenocarcinoma. Cell Physiol Biochem. 50:196–213. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Qu X, Li Y, Wang L, Yuan N, Ma M and Chen Y: lncRNA SNHG8 accelerates proliferation and inhibits apoptosis in HPV-induced cervical cancer through recruiting EZH2 to epigenetically silence RECK expression. J Cell Biochem. 121:4120–4129. 2020. View Article : Google Scholar : PubMed/NCBI

32 

Dikic I and Elazar Z: Mechanism and medical implications of mammalian autophagy. Nat Rev Mol Cell Biol. 19:349–364. 2018. View Article : Google Scholar : PubMed/NCBI

33 

Kroemer G: Autophagy: A druggable process that is deregulated in aging and human disease. J Clin Invest. 125:1–4. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Guo C, Peng X, Song L, Ying M, Wu Y, Chang R, Li J, Feng D, Zhan L and Zhan X: Autophagy promotes malignant migration and invasion via miR-224-5p/BCL2 in pancreatic mucinous cystadenocarcinoma MCC1 cells. Oncol Lett. 20:2762020. View Article : Google Scholar : PubMed/NCBI

35 

Cao QH, Liu F, Yang ZL, Fu XH, Yang ZH, Liu Q, Wang L, Wan XB and Fan XJ: Prognostic value of autophagy related proteins ULK1, Beclin 1, ATG3, ATG5, ATG7, ATG9, ATG10, ATG12, LC3B and p62/SQSTM1 in gastric cancer. Am J Transl Res. 8:3831–3847. 2016.PubMed/NCBI

36 

Yang L, Peng X, Jin H and Liu J: Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma. Gene. 697:94–102. 2019. View Article : Google Scholar : PubMed/NCBI

37 

Kang Y, Jia Y, Wang Q, Zhao Q, Song M, Ni R and Wang J: Long noncoding RNA KCNQ1OT1 promotes the progression of non-small cell lung cancer via regulating miR-204-5p/ATG3 axis. Onco Targets Ther. 12:10787–10797. 2019. View Article : Google Scholar : PubMed/NCBI

38 

Lin L, Liu X and Lv B: Long non-coding RNA MEG3 promotes autophagy and apoptosis of nasopharyngeal carcinoma cells via PTEN up-regulation by binding to microRNA-21. J Cell Mol Med. 25:61–72. 2021. View Article : Google Scholar : PubMed/NCBI

39 

Li XM, Jiao YY, Luan BH, Wu HX, Wang RR and Zhong J: Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway. Oncol Lett. 20:3552020. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He C, Fu Y, Chen Y and Li X: Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer. Oncol Lett 22: 577, 2021.
APA
He, C., Fu, Y., Chen, Y., & Li, X. (2021). Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer. Oncology Letters, 22, 577. https://doi.org/10.3892/ol.2021.12838
MLA
He, C., Fu, Y., Chen, Y., Li, X."Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer". Oncology Letters 22.2 (2021): 577.
Chicago
He, C., Fu, Y., Chen, Y., Li, X."Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer". Oncology Letters 22, no. 2 (2021): 577. https://doi.org/10.3892/ol.2021.12838
Copy and paste a formatted citation
x
Spandidos Publications style
He C, Fu Y, Chen Y and Li X: Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer. Oncol Lett 22: 577, 2021.
APA
He, C., Fu, Y., Chen, Y., & Li, X. (2021). Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer. Oncology Letters, 22, 577. https://doi.org/10.3892/ol.2021.12838
MLA
He, C., Fu, Y., Chen, Y., Li, X."Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer". Oncology Letters 22.2 (2021): 577.
Chicago
He, C., Fu, Y., Chen, Y., Li, X."Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer". Oncology Letters 22, no. 2 (2021): 577. https://doi.org/10.3892/ol.2021.12838
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team