Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1

  • Authors:
    • Qing Chen
    • Yidong Wang
    • Huimin Dang
    • Xiaoling Wu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Integrated Traditional Chinese and Western Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Article Number: 617
    |
    Published online on: June 24, 2021
       https://doi.org/10.3892/ol.2021.12878
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRs) serve a key role in carcinogenesis. miR‑148a‑3p has been demonstrated to act as a tumor suppressor in several tumors, such as epithelial ovarian cancer and esophageal cancer. However, to the best of our knowledge, the role of miR‑148a‑3p in cervical cancer remains unclear. In the present study, the expression levels of miR‑148a‑3p measured by reverse transcription‑quantitative PCR were significantly decreased in cervical cancer tissues compared with that in normal cervical tissues. Furthermore, overexpression of miR‑148a‑3p markedly suppressed the proliferation of cervical cancer cells. The luciferase reporter assay demonstrated that DNA methyltransferase 1 (DNMT1) was the target gene of miR‑148a‑3p and that its expression measured by western blotting was inhibited by miR‑148a‑3p in cervical cancer cells. Correlation analysis highlighted that the expression levels of the undifferentiated embryonic cell transcription factor‑1 (UTF1) were negatively associated with the expression levels of DNMT1 in cervical cancer tissues. Furthermore, DNMT1 knockdown increased the expression of UTF1 and decreased the methylation level of UTF1 promoter. These data demonstrated the expression levels of UTF1 were regulated by DNMT1 methylation in cervical cancer cells. Collectively, the results of the present study suggested that miR‑148a‑3p may inhibit the proliferation of cervical cancer cells by regulating the expression levels of DNMT1/UTF1, which provides potential therapeutic targets for cervical cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Small W Jr, Bacon MA, Bajaj A, Chuang LT, Fisher BJ, Harkenrider MM, Jhingran A, Kitchener HC, Mileshkin LR, Viswanathan AN and Gaffney DK: Cervical cancer: A global health crisis. Cancer. 123:2404–2412. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Wang J, Lv H, Xue Z, Wang L and Bai Z: Temporal trends of common female malignances on breast, cervical, and ovarian cancer mortality in japan, republic of Korea, and Singapore: Application of the age-period-cohort model. Biomed Res Int. 2018:53074592018.PubMed/NCBI

4 

Gupta S, Kumar P and Das BC: HPV: Molecular pathways and targets. Curr Probl Cancer. 42:161–174. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Drusco A and Croce CM: MicroRNAs and cancer: A long story for short RNAs. Adv Cancer Res. 135:1–24. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Fanini F and Fabbri M: Cancer-derived exosomic microRNAs shape the immune system within the tumor microenvironment: State of the art. Semin Cell Dev Biol. 67:23–28. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Costello A, Lao N, Clynes M and Barron N: Conditional knockdown of endogenous MicroRNAs in CHO cells using TET-ON-SanDI sponge vectors. Methods Mol Biol. 1603:87–100. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Höck J and Meister G: The Argonaute protein family. Genome Biol. 9:2102008. View Article : Google Scholar

9 

Lin X, Xiaoqin H, Jiayu C, Li F, Yue L and Ximing X: Long non-coding RNA miR143HG predicts good prognosis and inhibits tumor multiplication and metastasis by suppressing mitogen-activated protein kinase and Wnt signaling pathways in hepatocellular carcinoma. Hepatol Res. 49:902–918. 2019. View Article : Google Scholar : PubMed/NCBI

10 

Zhang M, Wang C and Mu L: Mir-148a induces apoptosis by upregulating bim expression in gastric cancer cells. Int J Clin Exp Med. 10:2791–2799. 2017.PubMed/NCBI

11 

Liu J, Si L and Tian H: MicroRNA-148a inhibits cell proliferation and cell cycle progression in lung adenocarcinoma via directly targeting transcription factor E2F3. Exp Ther Med. 16:5400–5409. 2018.PubMed/NCBI

12 

Xiao WD, Ao J, Huang Z, Lin SR, Peng L and Li Y: The effects of up-regulation of miR-148a on the expression of anti-oncogene ppENK, p16 and RASSF1A in pancreatic carcinoma AsPC-1 cells. Pancreatology. 16 (Suppl):S152016. View Article : Google Scholar

13 

Zhang H, Li Y, Huang Q, Ren X, Hu H, Sheng H and Lai M: MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer. Cell Death Differ. 18:1702–1710. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Wu XL and Zheng PS: Undifferentiated embryonic cell transcription factor-1 (UTF1) inhibits the growth of cervical cancer cells by transactivating p27 Kip1. Carcinogenesis. 34:1660–1668. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Bock C, Reither S, Mikeska T, Paulsen M, Walter J and Lengauer T: BiQ Analyzer: Visualization and quality control for DNA methylation data from bisulfite sequencing. Bioinformatics. 21:4067–4068. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Han M, Li J, Cao Y, Huang Y, Li W, Zhu H, Zhao Q, Han JJ, Wu Q, Li J, et al: A role for LSH in facilitating DNA methylation by DNMT1 through enhancing UHRF1 chromatin association. Nucleic Acids Res. 48:12116–12134. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Wei Y, Peng S, Wu M, Sachidanandam R, Tu Z, Zhang S, Falce C, Sobie EA, Lebeche D and Zhao Y: Multifaceted roles of miR-1s in repressing the fetal gene program in the heart. Cell Res. 24:278–292. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Yu X and Li Z: The role of microRNAs expression in laryngeal cancer. Oncotarget. 6:23297–23305. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Li Y, Deng X, Zeng X and Peng X: The role of Mir-148a in cancer. J Cancer. 7:1233–1241. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Zhao W, Zheng J, Wei G, Yang K, Wang G and Sun X: miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer. Oncol Lett. 18:2530–2536. 2019.PubMed/NCBI

22 

Zuo J, Xia J, Ju F, Yan J, Zhu A, Jin S, Shan T and Zhou H: MicroRNA-148a can regulate runt-related transcription factor 3 gene expression via modulation of DNA methyltransferase 1 in gastric cancer. Mol Cells. 35:313–319. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Pantazis G, Harter PN, Capper D, Kohlhof P, Mittelbronn M and Schittenhelm J: The embryonic stem cell factor UTF1 serves as a reliable diagnostic marker for germinomas. Pathology. 46:225–229. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Okuda A, Fukushima A, Nishimoto M, Orimo A, Yamagishi T, Nabeshima Y, Kuro-o M, Nabeshima Yi, Boon K, Keaveney M, et al: UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. EMBO J. 17:2019–2032. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Jin B and Robertson KD: DNA Methyltransferases, DNA damage repair, and cancer. Adv Exp Med Biol. 754:3–29. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Foltz G, Yoon JG, Lee H, Ryken TC, Sibenaller Z, Ehrich M, Hood L and Madan A: DNA methyltransferase-mediated transcriptional silencing in malignant glioma: A combined whole-genome microarray and promoter array analysis. Oncogene. 28:2667–2677. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Scopa CD, Zolota V and Kourea HP: Targeted pathways in breast cancer: Molecular and protein markers guiding therapeutic decisions. Curr Mol Pharmacol. 7:4–21. 2014.PubMed/NCBI

28 

Kumar R and Rao DN: Role of DNA Methyltransferases in epigenetic regulation in bacteria. Subcell Biochem. 61:81–102. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Subramaniam D, Thombre R, Dhar A and Anant S: DNA Methyltransferases: A novel target for prevention and therapy. Front Oncol. 4:80. 2014. View Article : Google Scholar : PubMed/NCBI

30 

You Y, Yang W, Wang Z, Zhu H, Li H, Lin C and Ran Y: Promoter hypermethylation contributes to the frequent suppression of the CDK10 gene in human nasopharyngeal carcinomas. Cell Oncol (Dordr). 36:323–331. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen Q, Wang Y, Dang H and Wu X: MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1. Oncol Lett 22: 617, 2021.
APA
Chen, Q., Wang, Y., Dang, H., & Wu, X. (2021). MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1. Oncology Letters, 22, 617. https://doi.org/10.3892/ol.2021.12878
MLA
Chen, Q., Wang, Y., Dang, H., Wu, X."MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1". Oncology Letters 22.2 (2021): 617.
Chicago
Chen, Q., Wang, Y., Dang, H., Wu, X."MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1". Oncology Letters 22, no. 2 (2021): 617. https://doi.org/10.3892/ol.2021.12878
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Q, Wang Y, Dang H and Wu X: MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1. Oncol Lett 22: 617, 2021.
APA
Chen, Q., Wang, Y., Dang, H., & Wu, X. (2021). MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1. Oncology Letters, 22, 617. https://doi.org/10.3892/ol.2021.12878
MLA
Chen, Q., Wang, Y., Dang, H., Wu, X."MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1". Oncology Letters 22.2 (2021): 617.
Chicago
Chen, Q., Wang, Y., Dang, H., Wu, X."MicroRNA‑148a‑3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1". Oncology Letters 22, no. 2 (2021): 617. https://doi.org/10.3892/ol.2021.12878
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team