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HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway

  • Authors:
    • Jianying Guo
    • Songbiao Zhu
    • Haiteng Deng
    • Renhua Xu
  • View Affiliations / Copyright

    Affiliations: School of Nursing, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Key Laboratory of Bioinformatics, Ministry of Education, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 630
    |
    Published online on: July 1, 2021
       https://doi.org/10.3892/ol.2021.12891
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Abstract

Colorectal cancer (CRC) is the fourth most lethal cancer in the world. Heat shock protein 60 (HSP60), a mitochondrial chaperone that maintains mitochondrial proteostasis, is highly expressed in tumors compared with in paracancerous tissues, suggesting that high HSP60 expression benefits tumor growth. To determine the effects of HSP60 expression on tumor progression, stable HSP60‑knockdown HCT116 cells were constructed in the present study, revealing that knockdown of HSP60 inhibited cell proliferation. Proteomic analysis demonstrated that mitochondrial proteins were downregulated, indicating that knockdown of HSP60 disrupted mitochondrial homeostasis. Metabolomic analysis demonstrated that cellular adenine levels were >30‑fold higher in HSP60‑knockdown cells than in control cells. It was further confirmed that elevated adenine activated the AMPK signaling pathway, which inhibited mTOR‑regulated protein synthesis to slow down cell proliferation. Overall, the current results provide a valuable resource for understanding mitochondrial function in CRC, suggesting that HSP60 may be a potential target for CRC intervention.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Fearon ER and Vogelstein B: A genetic model for colorectal tumorigenesis. Cell. 61:759–767. 1990. View Article : Google Scholar : PubMed/NCBI

3 

de la Chapelle A: Genetic predisposition to colorectal cancer. Nat Rev Cancer. 4:769–780. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Knowlton AA and Srivatsa U: Heat-shock protein 60 and cardiovascular disease: A paradoxical role. Future Cardiol. 4:151–161. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Hansen JJ, Durr A, Cournu-Rebeix I, Georgopoulos C, Ang D, Nielsen MN, Davoine CS, Brice A, Fontaine B, Gregersen N and Bross P: Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet. 70:1328–1332. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Grundtman C and Wick G: The autoimmune concept of atherosclerosis. Curr Opin Lipidol. 22:327–334. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Guo J, Li X, Zhang W, Chen Y, Zhu S, Chen L, Xu R, Lv Y, Wu D, Guo M, et al: HSP60-regulated mitochondrial proteostasis and protein translation promote tumor growth of ovarian cancer. Sci Rep. 9:126282019. View Article : Google Scholar : PubMed/NCBI

8 

Cappello F, Conway DME, Marasa L, Zummo G and Macario AJ: Hsp60 expression, new locations, functions and perspectives for cancer diagnosis and therapy. Cancer Biol Ther. 7:801–809. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Ghosh JC, Dohi T, Kang BH and Altieri DC: Hsp60 regulation of tumor cell apoptosis. J Biol Chem. 283:5188–5194. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Tsai YP, Yang MH, Huang CH, Chang SY, Chen PM, Liu CJ, Teng SC and Wu KJ: Interaction between HSP60 and beta-catenin promotes metastasis. Carcinogenesis. 30:1049–1057. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Ghosh JC, Siegelin MD, Dohi T and Altieri DC: Heat shock protein 60 regulation of the mitochondrial permeability transition pore in tumor cells. Cancer Res. 70:8988–8993. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Cappello F, Bellafiore M, Palma A, David S, Marciano V, Bartolotta T, Sciume C, Modica G, Farina F, Zummo G and Bucchieri F: 60KDa chaperonin (HSP60) is over-expressed during colorectal carcinogenesis. Eur J Histochem. 47:105–110. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Tang H, Chen Y, Liu X, Wang S, Lv Y, Wu D, Wang Q, Luo M and Deng H: Downregulation of HSP60 disrupts mitochondrial proteostasis to promote tumorigenesis and progression in clear cell renal cell carcinoma. Oncotarget. 7:38822–38834. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Teng R, Liu Z, Tang H, Zhang W, Chen Y, Xu R, Chen L, Song J, Liu X and Deng H: HSP60 silencing promotes Warburg-like phenotypes and switches the mitochondrial function from ATP production to biosynthesis in ccRCC cells. Redox Biol. 24:1012182019. View Article : Google Scholar : PubMed/NCBI

15 

Hjerpe E, Egyhazi S, Carlson J, Stolt MF, Schedvins K, Johansson H, Shoshan M and Avall-Lundqvist E: HSP60 predicts survival in advanced serous ovarian cancer. Int J Gynecol Cancer. 23:448–455. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Cappello F, Rappa F, David S, Anzalone R and Zummo G: Immunohistochemical evaluation of PCNA, p53, HSP60, HSP10 and MUC-2 presence and expression in prostate carcinogenesis. Anticancer Res. 23:1325–1331. 2003.PubMed/NCBI

17 

Boston University Research Support, . Carbon Dioxide Euthanasia for Rats and Mice (BU ASC Guidelines). Boston University Research Support; Boston, MA: 2019, https://www.bu.edu/researchsupport/compliance/animal-care/working-with-animals/euthanasia/carbon-dioxide-euthanasia-for-rats-and-mice/Revised May 2019.

18 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45:W98–W102. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Sánchez-González C, Nuevo-Tapioles C, Herrero Martín JC, Pereira MP, Serrano Sanz S, Ramírez de Molina A, Cuezva JM and Formentini L: Dysfunctional oxidative phosphorylation shunts branched-chain amino acid catabolism onto lipogenesis in skeletal muscle. EMBO J. 39:e1038122020. View Article : Google Scholar

20 

Zaborske JM, Narasimhan J, Jiang L, Wek SA, Dittmar KA, Freimoser F, Pan T and Wek RC: Genome-wide analysis of tRNA charging and activation of the eIF2 kinase Gcn2p. J Biol Chem. 284:25254–25267. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Mills GC, Schmalstieg FC, Trimmer KB, Goldman AS and Goldblum RM: Purine metabolism in adenosine deaminase deficiency. Proc Natl Acad Sci USA. 73:2867–2871. 1976. View Article : Google Scholar : PubMed/NCBI

22 

Jeon SM: Regulation and function of AMPK in physiology and diseases. Exp Mol Med. 48:e2452016. View Article : Google Scholar : PubMed/NCBI

23 

Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, et al: Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis. 9:1612018. View Article : Google Scholar : PubMed/NCBI

24 

Tang H, Li J, Liu X, Wang G, Luo M and Deng H: Down-regulation of HSP60 suppresses the proliferation of Glioblastoma cells via the ROS/AMPK/mTOR pathway. Sci Rep. 6:283882016. View Article : Google Scholar : PubMed/NCBI

25 

Vocka M, Langer D, Fryba V, Petrtyl J, Hanus T, Kalousova M, Zima T and Petruzelka L: Novel serum markers HSP60, CHI3L1, and IGFBP-2 in metastatic colorectal cancer. Oncol Lett. 18:6284–6292. 2019.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Zhu S, Deng H and Xu R: HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway. Oncol Lett 22: 630, 2021.
APA
Guo, J., Zhu, S., Deng, H., & Xu, R. (2021). HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway. Oncology Letters, 22, 630. https://doi.org/10.3892/ol.2021.12891
MLA
Guo, J., Zhu, S., Deng, H., Xu, R."HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway". Oncology Letters 22.2 (2021): 630.
Chicago
Guo, J., Zhu, S., Deng, H., Xu, R."HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway". Oncology Letters 22, no. 2 (2021): 630. https://doi.org/10.3892/ol.2021.12891
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Zhu S, Deng H and Xu R: HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway. Oncol Lett 22: 630, 2021.
APA
Guo, J., Zhu, S., Deng, H., & Xu, R. (2021). HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway. Oncology Letters, 22, 630. https://doi.org/10.3892/ol.2021.12891
MLA
Guo, J., Zhu, S., Deng, H., Xu, R."HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway". Oncology Letters 22.2 (2021): 630.
Chicago
Guo, J., Zhu, S., Deng, H., Xu, R."HSP60‑knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway". Oncology Letters 22, no. 2 (2021): 630. https://doi.org/10.3892/ol.2021.12891
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